CD3D_MOUSE
ID CD3D_MOUSE Reviewed; 173 AA.
AC P04235;
DT 20-MAR-1987, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-1987, sequence version 1.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=T-cell surface glycoprotein CD3 delta chain;
DE AltName: Full=T-cell receptor T3 delta chain;
DE AltName: CD_antigen=CD3d;
DE Flags: Precursor;
GN Name=Cd3d; Synonyms=T3d;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2939461; DOI=10.1073/pnas.83.9.2944;
RA van den Elsen P., Georgopoulos K., Shepley B.-A., Orkin S., Terhorst C.;
RT "Exon/intron organization of the genes coding for the delta chains of the
RT human and murine T-cell receptor/T3 complex.";
RL Proc. Natl. Acad. Sci. U.S.A. 83:2944-2948(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18.
RC STRAIN=BALB/cJ; TISSUE=Liver;
RX PubMed=2827170; DOI=10.1073/pnas.84.24.9131;
RA Saito H., Koyama T., Georgopoulos K., Clevers H., Haser W.G., Lebien T.,
RA Tonegawa S., Terhorst C.;
RT "Close linkage of the mouse and human CD3 gamma- and delta-chain genes
RT suggests that their transcription is controlled by common regulatory
RT elements.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:9131-9134(1987).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=10935641; DOI=10.1038/35019102;
RA Delgado P., Fernandez E., Dave V., Kappes D., Alarcon B.;
RT "CD3delta couples T-cell receptor signalling to ERK activation and
RT thymocyte positive selection.";
RL Nature 406:426-430(2000).
RN [4]
RP SUBUNIT, AND TISSUE SPECIFICITY.
RX PubMed=15459203; DOI=10.1074/jbc.m406145200;
RA Pan Q., Gollapudi A.S., Dave V.P.;
RT "Biochemical evidence for the presence of a single CD3delta and CD3gamma
RT chain in the surface T cell receptor/CD3 complex.";
RL J. Biol. Chem. 279:51068-51074(2004).
CC -!- FUNCTION: Part of the TCR-CD3 complex present on T-lymphocyte cell
CC surface that plays an essential role in adaptive immune response. When
CC antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-
CC mediated signals are transmitted across the cell membrane by the CD3
CC chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor
CC tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
CC Upon TCR engagement, these motifs become phosphorylated by Src family
CC protein tyrosine kinases LCK and FYN, resulting in the activation of
CC downstream signaling pathways. In addition of this role of signal
CC transduction in T-cell activation, CD3D plays an essential role in
CC thymocyte differentiation. Indeed, participates in correct
CC intracellular TCR-CD3 complex assembly and surface expression. In
CC absence of a functional TCR-CD3 complex, thymocytes are unable to
CC differentiate properly (PubMed:10935641). Interacts with CD4 and CD8
CC and thus serves to establish a functional link between the TCR and
CC coreceptors CD4 and CD8, which is needed for activation and positive
CC selection of CD4 or CD8 T-cells. {ECO:0000250|UniProtKB:P04234,
CC ECO:0000269|PubMed:10935641}.
CC -!- SUBUNIT: The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E
CC heterodimers that preferentially associate with TCRalpha and TCRbeta,
CC respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers.
CC In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3
CC complex. Alternatively, TCRalpha and TCRbeta can be replaced by
CC TCRgamma and TCRdelta. Interacts with coreceptors CD4 and CD8.
CC {ECO:0000250|UniProtKB:P04234, ECO:0000269|PubMed:15459203}.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- PTM: Phosphorylated on Tyr residues after T-cell receptor triggering by
CC LCK in association with CD4/CD8. {ECO:0000250|UniProtKB:P04234}.
CC -!- DISRUPTION PHENOTYPE: Thymocyte development in mice lacking CD3D is
CC arrested at the double-positive stage with markedly diminished TCR
CC expression. Thymocytes are defective in the induction of ERK pathway
CC upon TCR engagement, whereas activation of other MAP kinases is
CC unaffected. {ECO:0000269|PubMed:10935641}.
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DR EMBL; M12729; AAA37268.1; -; Genomic_DNA.
DR EMBL; M12728; AAA37268.1; JOINED; Genomic_DNA.
DR EMBL; M18222; AAA37399.1; -; Genomic_DNA.
DR CCDS; CCDS23124.1; -.
DR PIR; A02246; RWMSD2.
DR RefSeq; NP_038515.3; NM_013487.3.
DR AlphaFoldDB; P04235; -.
DR SMR; P04235; -.
DR CORUM; P04235; -.
DR ELM; P04235; -.
DR IntAct; P04235; 1.
DR STRING; 10090.ENSMUSP00000034602; -.
DR GlyGen; P04235; 3 sites.
DR iPTMnet; P04235; -.
DR PhosphoSitePlus; P04235; -.
DR SwissPalm; P04235; -.
DR EPD; P04235; -.
DR PaxDb; P04235; -.
DR PRIDE; P04235; -.
DR ProteomicsDB; 279967; -.
DR ABCD; P04235; 1 sequenced antibody.
DR Antibodypedia; 4581; 833 antibodies from 41 providers.
DR DNASU; 12500; -.
DR Ensembl; ENSMUST00000034602; ENSMUSP00000034602; ENSMUSG00000032094.
DR GeneID; 12500; -.
DR KEGG; mmu:12500; -.
DR UCSC; uc009pey.2; mouse.
DR CTD; 915; -.
DR MGI; MGI:88331; Cd3d.
DR VEuPathDB; HostDB:ENSMUSG00000032094; -.
DR eggNOG; ENOG502S4XC; Eukaryota.
DR GeneTree; ENSGT00940000153312; -.
DR HOGENOM; CLU_115449_0_0_1; -.
DR InParanoid; P04235; -.
DR OMA; YQPLRDH; -.
DR OrthoDB; 1466902at2759; -.
DR PhylomeDB; P04235; -.
DR TreeFam; TF335892; -.
DR Reactome; R-MMU-198933; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
DR Reactome; R-MMU-202424; Downstream TCR signaling.
DR Reactome; R-MMU-202427; Phosphorylation of CD3 and TCR zeta chains.
DR Reactome; R-MMU-202430; Translocation of ZAP-70 to Immunological synapse.
DR Reactome; R-MMU-202433; Generation of second messenger molecules.
DR Reactome; R-MMU-389948; PD-1 signaling.
DR Reactome; R-MMU-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-MMU-8856828; Clathrin-mediated endocytosis.
DR BioGRID-ORCS; 12500; 5 hits in 73 CRISPR screens.
DR ChiTaRS; Cd3d; mouse.
DR PRO; PR:P04235; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; P04235; protein.
DR Bgee; ENSMUSG00000032094; Expressed in peripheral lymph node and 62 other tissues.
DR ExpressionAtlas; P04235; baseline and differential.
DR Genevisible; P04235; MM.
DR GO; GO:0042105; C:alpha-beta T cell receptor complex; IDA:MGI.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0042101; C:T cell receptor complex; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IBA:GO_Central.
DR GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0045059; P:positive thymic T cell selection; IMP:UniProtKB.
DR GO; GO:0065003; P:protein-containing complex assembly; NAS:UniProtKB.
DR GO; GO:0042110; P:T cell activation; NAS:UniProtKB.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR015484; CD3_esu/gsu/dsu.
DR InterPro; IPR015485; CD3D.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032052; Ig_4.
DR InterPro; IPR003110; Phos_immunorcpt_sig_ITAM.
DR PANTHER; PTHR10570; PTHR10570; 1.
DR PANTHER; PTHR10570:SF5; PTHR10570:SF5; 1.
DR Pfam; PF16680; Ig_4; 1.
DR Pfam; PF02189; ITAM; 1.
DR SMART; SM00077; ITAM; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR PROSITE; PS51055; ITAM_1; 1.
PE 1: Evidence at protein level;
KW Adaptive immunity; Disulfide bond; Glycoprotein; Immunity; Membrane;
KW Phosphoprotein; Receptor; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..21
FT CHAIN 22..173
FT /note="T-cell surface glycoprotein CD3 delta chain"
FT /id="PRO_0000016489"
FT TOPO_DOM 22..105
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..173
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 138..166
FT /note="ITAM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00379"
FT MOD_RES 149
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P04234,
FT ECO:0000255|PROSITE-ProRule:PRU00379"
FT MOD_RES 160
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P04234,
FT ECO:0000255|PROSITE-ProRule:PRU00379"
FT CARBOHYD 38
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 55
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 74
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 37..73
FT /evidence="ECO:0000250"
SQ SEQUENCE 173 AA; 19032 MW; EEEC182BDF2AFB6B CRC64;
MEHSGILASL ILIAVLPQGS PFKIQVTEYE DKVFVTCNTS VMHLDGTVEG WFAKNKTLNL
GKGVLDPRGI YLCNGTEQLA KVVSSVQVHY RMCQNCVELD SGTMAGVIFI DLIATLLLAL
GVYCFAGHET GRPSGAAEVQ ALLKNEQLYQ PLRDREDTQY SRLGGNWPRN KKS
