CD3G_MOUSE
ID CD3G_MOUSE Reviewed; 182 AA.
AC P11942;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=T-cell surface glycoprotein CD3 gamma chain;
DE AltName: Full=T-cell receptor T3 gamma chain;
DE AltName: CD_antigen=CD3g;
DE Flags: Precursor;
GN Name=Cd3g;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3655657; DOI=10.1084/jem.166.4.1186;
RA Haser W.G., Saito H., Koyama T., Tonegawa S.;
RT "Cloning and sequencing of murine T3 gamma cDNA from a subtractive cDNA
RT library.";
RL J. Exp. Med. 166:1186-1191(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2952720;
RA Krissansen G.W., Owen M.J., Fink P.J., Crumpton M.J.;
RT "Molecular cloning of the cDNA encoding the T3 gamma subunit of the mouse
RT T3/T cell antigen receptor complex.";
RL J. Immunol. 138:3513-3518(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2827170; DOI=10.1073/pnas.84.24.9131;
RA Saito H., Koyama T., Georgopoulos K., Clevers H., Haser W.G., Lebien T.,
RA Tonegawa S., Terhorst C.;
RT "Close linkage of the mouse and human CD3 gamma- and delta-chain genes
RT suggests that their transcription is controlled by common regulatory
RT elements.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:9131-9134(1987).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=9524111; DOI=10.1093/emboj/17.7.1871;
RA Haks M.C., Krimpenfort P., Borst J., Kruisbeek A.M.;
RT "The CD3gamma chain is essential for development of both the TCRalphabeta
RT and TCRgammadelta lineages.";
RL EMBO J. 17:1871-1882(1998).
RN [6]
RP FUNCTION.
RX PubMed=25920998; DOI=10.1093/intimm/dxv022;
RA Lauritsen J.P., Boding L., Buus T.B., Kongsbak M., Levring T.B., Rode A.K.,
RA Bonefeld C.M., Geisler C.;
RT "Fine-tuning of T-cell development by the CD3gamma di-leucine-based TCR-
RT sorting motif.";
RL Int. Immunol. 27:393-404(2015).
CC -!- FUNCTION: Part of the TCR-CD3 complex present on T-lymphocyte cell
CC surface that plays an essential role in adaptive immune response. When
CC antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-
CC mediated signals are transmitted across the cell membrane by the CD3
CC chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor
CC tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
CC Upon TCR engagement, these motifs become phosphorylated by Src family
CC protein tyrosine kinases LCK and FYN, resulting in the activation of
CC downstream signaling pathways. In addition to this role of signal
CC transduction in T-cell activation, CD3G plays an essential role in the
CC dynamic regulation of TCR expression at the cell surface. Indeed,
CC constitutive TCR cycling is dependent on the di-leucine-based (diL)
CC receptor-sorting motif present in CD3G (PubMed:25920998).
CC {ECO:0000250|UniProtKB:P09693, ECO:0000269|PubMed:25920998,
CC ECO:0000269|PubMed:9524111}.
CC -!- SUBUNIT: The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E
CC heterodimers that preferentially associate with TCRalpha and TCRbeta,
CC respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers.
CC In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3
CC complex. Alternatively, TCRalpha and TCRbeta can be replaced by
CC TCRgamma and TCRdelta. {ECO:0000250|UniProtKB:P09693}.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- PTM: Phosphorylated on Tyr residues after T-cell receptor triggering by
CC LCK in association with CD4/CD8. Phosphorylated also by PKC; leading to
CC the TCR complex down-regulation. {ECO:0000250|UniProtKB:P09693}.
CC -!- DISRUPTION PHENOTYPE: The absence of CD3G results in a severe reduction
CC in the level of the TCR-CD3 at the cell surface of thymocytes and
CC peripheral T cells. The development of both the TCRalphabeta and
CC TCRgammadelta lineages are affected by the absence of CD3G.
CC {ECO:0000269|PubMed:9524111}.
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DR EMBL; Y00635; CAA68667.1; -; mRNA.
DR EMBL; M58149; AAA63289.1; ALT_SEQ; mRNA.
DR EMBL; M18227; AAA37400.1; -; Genomic_DNA.
DR EMBL; M18222; AAA37400.1; JOINED; Genomic_DNA.
DR EMBL; M18223; AAA37400.1; JOINED; Genomic_DNA.
DR EMBL; M18224; AAA37400.1; JOINED; Genomic_DNA.
DR EMBL; M18225; AAA37400.1; JOINED; Genomic_DNA.
DR EMBL; M18226; AAA37400.1; JOINED; Genomic_DNA.
DR EMBL; BC027528; AAH27528.1; -; mRNA.
DR CCDS; CCDS23123.1; -.
DR PIR; A39952; A28508.
DR RefSeq; NP_033980.1; NM_009850.2.
DR PDB; 1JBJ; NMR; -; A=23-103.
DR PDBsum; 1JBJ; -.
DR AlphaFoldDB; P11942; -.
DR SMR; P11942; -.
DR CORUM; P11942; -.
DR ELM; P11942; -.
DR IntAct; P11942; 1.
DR STRING; 10090.ENSMUSP00000002101; -.
DR GlyGen; P11942; 1 site.
DR iPTMnet; P11942; -.
DR PhosphoSitePlus; P11942; -.
DR EPD; P11942; -.
DR jPOST; P11942; -.
DR PaxDb; P11942; -.
DR PRIDE; P11942; -.
DR ProteomicsDB; 281268; -.
DR ABCD; P11942; 1 sequenced antibody.
DR Antibodypedia; 4306; 359 antibodies from 41 providers.
DR DNASU; 12502; -.
DR Ensembl; ENSMUST00000002101; ENSMUSP00000002101; ENSMUSG00000002033.
DR GeneID; 12502; -.
DR KEGG; mmu:12502; -.
DR UCSC; uc009pex.2; mouse.
DR CTD; 917; -.
DR MGI; MGI:88333; Cd3g.
DR VEuPathDB; HostDB:ENSMUSG00000002033; -.
DR eggNOG; ENOG502S4XC; Eukaryota.
DR GeneTree; ENSGT00940000153312; -.
DR HOGENOM; CLU_115449_0_0_1; -.
DR InParanoid; P11942; -.
DR OMA; DRENDQY; -.
DR OrthoDB; 1466902at2759; -.
DR PhylomeDB; P11942; -.
DR TreeFam; TF335892; -.
DR Reactome; R-MMU-198933; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
DR Reactome; R-MMU-202424; Downstream TCR signaling.
DR Reactome; R-MMU-202427; Phosphorylation of CD3 and TCR zeta chains.
DR Reactome; R-MMU-202430; Translocation of ZAP-70 to Immunological synapse.
DR Reactome; R-MMU-202433; Generation of second messenger molecules.
DR Reactome; R-MMU-2029481; FCGR activation.
DR Reactome; R-MMU-2029482; Regulation of actin dynamics for phagocytic cup formation.
DR Reactome; R-MMU-2029485; Role of phospholipids in phagocytosis.
DR Reactome; R-MMU-389948; PD-1 signaling.
DR Reactome; R-MMU-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-MMU-8856828; Clathrin-mediated endocytosis.
DR BioGRID-ORCS; 12502; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Cd3g; mouse.
DR EvolutionaryTrace; P11942; -.
DR PRO; PR:P11942; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; P11942; protein.
DR Bgee; ENSMUSG00000002033; Expressed in thymus and 59 other tissues.
DR ExpressionAtlas; P11942; baseline and differential.
DR Genevisible; P11942; MM.
DR GO; GO:0042105; C:alpha-beta T cell receptor complex; IDA:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; ISS:UniProtKB.
DR GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0007163; P:establishment or maintenance of cell polarity; ISS:UniProtKB.
DR GO; GO:0045059; P:positive thymic T cell selection; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; ISS:UniProtKB.
DR GO; GO:0070228; P:regulation of lymphocyte apoptotic process; ISS:UniProtKB.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR015484; CD3_esu/gsu/dsu.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032052; Ig_4.
DR InterPro; IPR003598; Ig_sub2.
DR InterPro; IPR003110; Phos_immunorcpt_sig_ITAM.
DR PANTHER; PTHR10570; PTHR10570; 1.
DR Pfam; PF16680; Ig_4; 1.
DR Pfam; PF02189; ITAM; 1.
DR SMART; SM00408; IGc2; 1.
DR SMART; SM00077; ITAM; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR PROSITE; PS51055; ITAM_1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Adaptive immunity; Disulfide bond; Glycoprotein; Immunity;
KW Immunoglobulin domain; Membrane; Phosphoprotein; Receptor;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT CHAIN 23..182
FT /note="T-cell surface glycoprotein CD3 gamma chain"
FT /id="PRO_0000014617"
FT TOPO_DOM 23..116
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..182
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 37..94
FT /note="Ig-like"
FT DOMAIN 149..177
FT /note="ITAM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00379"
FT MOD_RES 145
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P09693"
FT MOD_RES 148
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P09693"
FT CARBOHYD 66
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 46..87
FT /evidence="ECO:0000250"
FT STRAND 37..39
FT /evidence="ECO:0007829|PDB:1JBJ"
FT STRAND 41..45
FT /evidence="ECO:0007829|PDB:1JBJ"
FT STRAND 47..49
FT /evidence="ECO:0007829|PDB:1JBJ"
FT STRAND 53..57
FT /evidence="ECO:0007829|PDB:1JBJ"
FT STRAND 73..76
FT /evidence="ECO:0007829|PDB:1JBJ"
FT STRAND 82..91
FT /evidence="ECO:0007829|PDB:1JBJ"
FT STRAND 93..100
FT /evidence="ECO:0007829|PDB:1JBJ"
SQ SEQUENCE 182 AA; 20234 MW; 7F9D8D44A5C360D5 CRC64;
MEQRKGLAGL FLVISLLQGT VAQTNKAKNL VQVDGSRGDG SVLLTCGLTD KTIKWLKDGS
IISPLNATKN TWNLGNNAKD PRGTYQCQGA KETSNPLQVY YRMCENCIEL NIGTISGFIF
AEVISIFFLA LGVYLIAGQD GVRQSRASDK QTLLQNEQLY QPLKDREYDQ YSHLQGNQLR
KK
