CD40L_CHICK
ID CD40L_CHICK Reviewed; 272 AA.
AC Q9I8D8;
DT 06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 21-NOV-2003, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=CD40 ligand;
DE Short=CD40-L;
DE AltName: Full=Tumor necrosis factor ligand superfamily member 5;
DE Contains:
DE RecName: Full=CD40 ligand, membrane form;
DE Contains:
DE RecName: Full=CD40 ligand, soluble form {ECO:0000250|UniProtKB:P29965};
DE Short=sCD40L {ECO:0000250|UniProtKB:P29965};
GN Name=CD40LG; Synonyms=CD40L, TNFSF5;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=White leghorn; TISSUE=Spleen;
RA Tregaskes C.A., Young J.R., Burnside J.;
RT "Cloning of a putative chicken CD40 ligand.";
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cytokine that acts as a ligand to CD40/TNFRSF5 (By
CC similarity). Costimulates T-cell proliferation and cytokine production
CC (By similarity). Induces the activation of NF-kappa-B (By similarity).
CC Mediates B-cell proliferation in the absence of co-stimulus as well as
CC IgE production in the presence of IL4 (By similarity). Involved in
CC immunoglobulin class switching (By similarity).
CC {ECO:0000250|UniProtKB:P27548, ECO:0000250|UniProtKB:P29965}.
CC -!- FUNCTION: [CD40 ligand, soluble form]: Acts as a ligand for integrins,
CC specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40
CC receptor are required for activation of CD40-CD40LG signaling, which
CC have cell-type dependent effects, such as B-cell activation, NF-kappa-B
CC signaling and anti-apoptotic signaling. {ECO:0000250|UniProtKB:P29965}.
CC -!- SUBUNIT: Homotrimer (By similarity). Homotrimer (By similarity).
CC Interacts with CD28 (By similarity). CD40 ligand, soluble form: Exists
CC as either a monomer or a homotrimer (By similarity). Forms a ternary
CC complex between CD40 and integrins for CD40-CD40LG signaling (By
CC similarity). {ECO:0000250|UniProtKB:P29965}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P29965};
CC Single-pass type II membrane protein {ECO:0000250|UniProtKB:P29965}.
CC Cell surface {ECO:0000250|UniProtKB:P29965}.
CC -!- SUBCELLULAR LOCATION: [CD40 ligand, soluble form]: Secreted
CC {ECO:0000250|UniProtKB:P29965}. Note=Release of soluble CD40L from
CC platelets is partially regulated by GP IIb/IIIa, actin polymerization,
CC and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
CC {ECO:0000250|UniProtKB:P29965}.
CC -!- PTM: The soluble form derives from the membrane form by proteolytic
CC processing. {ECO:0000250|UniProtKB:P29965}.
CC -!- SIMILARITY: Belongs to the tumor necrosis factor family. {ECO:0000305}.
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DR EMBL; AJ243435; CAB95748.2; -; mRNA.
DR RefSeq; NP_990064.1; NM_204733.1.
DR AlphaFoldDB; Q9I8D8; -.
DR SMR; Q9I8D8; -.
DR STRING; 9031.ENSGALP00000010340; -.
DR PaxDb; Q9I8D8; -.
DR Ensembl; ENSGALT00000010354; ENSGALP00000010340; ENSGALG00000006415.
DR GeneID; 395485; -.
DR KEGG; gga:395485; -.
DR CTD; 959; -.
DR VEuPathDB; HostDB:geneid_395485; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01050000244878; -.
DR HOGENOM; CLU_093203_0_0_1; -.
DR InParanoid; Q9I8D8; -.
DR OMA; YLHIPKE; -.
DR OrthoDB; 1146113at2759; -.
DR PhylomeDB; Q9I8D8; -.
DR Reactome; R-GGA-198933; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
DR Reactome; R-GGA-5668541; TNFR2 non-canonical NF-kB pathway.
DR Reactome; R-GGA-5676594; TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway.
DR PRO; PR:Q9I8D8; -.
DR Proteomes; UP000000539; Chromosome 4.
DR Bgee; ENSGALG00000006415; Expressed in spleen and 8 other tissues.
DR ExpressionAtlas; Q9I8D8; baseline and differential.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005174; F:CD40 receptor binding; ISS:UniProtKB.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0005164; F:tumor necrosis factor receptor binding; IEA:InterPro.
DR GO; GO:0042100; P:B cell proliferation; ISS:UniProtKB.
DR GO; GO:0006955; P:immune response; IEA:InterPro.
DR GO; GO:0006954; P:inflammatory response; ISS:UniProtKB.
DR GO; GO:0030168; P:platelet activation; ISS:UniProtKB.
DR CDD; cd00184; TNF; 1.
DR Gene3D; 2.60.120.40; -; 1.
DR InterPro; IPR003263; CD40L.
DR InterPro; IPR021184; TNF_CS.
DR InterPro; IPR006052; TNF_dom.
DR InterPro; IPR008983; Tumour_necrosis_fac-like_dom.
DR PANTHER; PTHR11471:SF5; PTHR11471:SF5; 1.
DR Pfam; PF00229; TNF; 1.
DR PIRSF; PIRSF016527; TNF_5; 1.
DR PRINTS; PR01702; CD40LIGAND.
DR SMART; SM00207; TNF; 1.
DR SUPFAM; SSF49842; SSF49842; 1.
DR PROSITE; PS00251; TNF_1; 1.
DR PROSITE; PS50049; TNF_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytokine; Disulfide bond; Glycoprotein; Membrane;
KW Reference proteome; Secreted; Signal-anchor; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..272
FT /note="CD40 ligand, membrane form"
FT /id="PRO_0000034496"
FT CHAIN 111..272
FT /note="CD40 ligand, soluble form"
FT /evidence="ECO:0000250|UniProtKB:P29965"
FT /id="PRO_0000034497"
FT TOPO_DOM 1..23
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 24..44
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 45..272
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT SITE 110..111
FT /note="Cleavage"
FT /evidence="ECO:0000250"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 146
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 251
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 190..229
FT /evidence="ECO:0000255"
SQ SEQUENCE 272 AA; 30833 MW; 8CD0338A924E044B CRC64;
MNEAYSPAAP RPMGSTSPST MKMFMCFLSV FMVVQTIGTV LFCLYLHMKM DKMEEVLSLN
EDYIFLRKVQ KCQTGEDQKS TLLDCEKVLK GFQDLQCKDR TASEELPKFE MHRGHEHPHL
KSRNETSVAE EKRQPIATHL AGVKSNTTVR VLKWMTTSYA PTSSLISYHE GKLKVEKAGL
YYIYSQVSFC TKAAASAPFT LYIYLYLPME EDRLLMKGLD THSTSTALCE LQSIREGGVF
ELRQGDMVFV NVTDSTAVNV NPGNTYFGMF KL
