CD68_MOUSE
ID CD68_MOUSE Reviewed; 326 AA.
AC P31996; O54688; O70321; Q3S4A9; Q5F2A8; Q9DD15;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Macrosialin;
DE AltName: CD_antigen=CD68;
DE Flags: Precursor;
GN Name=Cd68;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
RC STRAIN=BALB/cJ; TISSUE=Macrophage;
RX PubMed=8486654; DOI=10.1016/s0021-9258(18)98400-0;
RA Holness C.L., da Silva R.P., Fawcett J., Gordon S., Simmons D.L.;
RT "Macrosialin, a mouse macrophage-restricted glycoprotein, is a member of
RT the lamp/lgp family.";
RL J. Biol. Chem. 268:9661-9666(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ;
RX PubMed=9653646; DOI=10.1006/geno.1998.5327;
RA Jiang Z., Shih D.M., Xia Y.R., Lusis A.J., de Beer F.C.,
RA de Villiers W.J.S., van der Westhuyzen D.R., de Beer M.C.;
RT "Structure, organization, and chromosomal mapping of the gene encoding
RT macrosialin, a macrophage-restricted protein.";
RL Genomics 50:199-205(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RX PubMed=9806844; DOI=10.1006/geno.1998.5546;
RA Greaves D.R., Quinn C.M., Seldin M.F., Gordon S.;
RT "Functional comparison of the murine macrosialin and human CD68 promoters
RT in macrophage and nonmacrophage cell lines.";
RL Genomics 54:165-168(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9479000; DOI=10.1074/jbc.273.9.5389;
RA Li A.C., Guidez F.R.B., Collier J.G., Glass C.K.;
RT "The macrosialin promoter directs high levels of transcriptional activity
RT in macrophages dependent on combinatorial interactions between PU.1 and c-
RT Jun.";
RL J. Biol. Chem. 273:5389-5399(1998).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129;
RX PubMed=10524236; DOI=10.1016/s0378-1119(99)00301-7;
RA Miyashita A., Shimizu N., Endo N., Hanyuu T., Ishii N., Ito K., Itoh Y.,
RA Shirai M., Nakajima T., Odani S., Kuwano R.;
RT "Five different genes, Eif4a1, Cd68, Supl15h, Sox15 and Fxr2h, are
RT clustered in a 40 kb region of mouse chromosome 11.";
RL Gene 237:53-60(1999).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Dendritic cell, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 33-203 (ISOFORMS LONG/SHORT).
RC STRAIN=129/Sv; TISSUE=Brain;
RX PubMed=16521125; DOI=10.1002/jnr.20798;
RA Denny C.A., Kasperzyk J.L., Gorham K.N., Bronson R.T., Seyfried T.N.;
RT "Influence of caloric restriction on motor behavior, longevity, and brain
RT lipid composition in Sandhoff disease mice.";
RL J. Neurosci. Res. 83:1028-1038(2006).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Could play a role in phagocytic activities of tissue
CC macrophages, both in intracellular lysosomal metabolism and
CC extracellular cell-cell and cell-pathogen interactions. Binds to
CC tissue- and organ-specific lectins or selectins, allowing homing of
CC macrophage subsets to particular sites. Rapid recirculation of CD68
CC from endosomes and lysosomes to the plasma membrane may allow
CC macrophages to crawl over selectin-bearing substrates or other cells.
CC -!- SUBCELLULAR LOCATION: [Isoform Long]: Endosome membrane; Single-pass
CC type I membrane protein. Lysosome membrane; Single-pass type I membrane
CC protein.
CC -!- SUBCELLULAR LOCATION: [Isoform Short]: Cell membrane; Single-pass type
CC I membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Long;
CC IsoId=P31996-1; Sequence=Displayed;
CC Name=Short;
CC IsoId=P31996-2; Sequence=VSP_003043;
CC -!- TISSUE SPECIFICITY: Expressed in tissue macrophages and to a lesser
CC extent in dendritic cells.
CC -!- PTM: N- and O-glycosylated.
CC -!- SIMILARITY: Belongs to the LAMP family. {ECO:0000255|PROSITE-
CC ProRule:PRU00740}.
CC -!- CAUTION: CD68 is a commonly used marker for macrophages. However, a
CC number of studies in human have shown that CD68 antibodies react with
CC other hematopoietic and non-hematopoietic cell types, suggesting that
CC CD68 may not be a macrophage-specific antigen. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA23738.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X68273; CAA48334.1; -; mRNA.
DR EMBL; AF022651; AAC40151.1; -; Genomic_DNA.
DR EMBL; AF045554; AAC15685.1; -; Genomic_DNA.
DR EMBL; AF039399; AAC40056.1; -; Genomic_DNA.
DR EMBL; AB009287; BAA23738.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK002264; BAB21975.1; -; mRNA.
DR EMBL; AK170443; BAE41801.1; -; mRNA.
DR EMBL; AL603707; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC021637; AAH21637.1; -; mRNA.
DR EMBL; DQ167574; ABA02181.1; -; mRNA.
DR CCDS; CCDS70216.1; -. [P31996-1]
DR PIR; A46676; A46676.
DR RefSeq; NP_001277987.1; NM_001291058.1. [P31996-1]
DR AlphaFoldDB; P31996; -.
DR SMR; P31996; -.
DR BioGRID; 198607; 1.
DR STRING; 10090.ENSMUSP00000104294; -.
DR GlyGen; P31996; 9 sites.
DR iPTMnet; P31996; -.
DR PhosphoSitePlus; P31996; -.
DR SwissPalm; P31996; -.
DR MaxQB; P31996; -.
DR PaxDb; P31996; -.
DR PeptideAtlas; P31996; -.
DR PRIDE; P31996; -.
DR ProteomicsDB; 283750; -. [P31996-1]
DR ProteomicsDB; 283751; -. [P31996-2]
DR ABCD; P31996; 2 sequenced antibodies.
DR DNASU; 12514; -.
DR Ensembl; ENSMUST00000018918; ENSMUSP00000018918; ENSMUSG00000018774. [P31996-1]
DR GeneID; 12514; -.
DR KEGG; mmu:12514; -.
DR UCSC; uc007jqy.2; mouse. [P31996-1]
DR CTD; 968; -.
DR MGI; MGI:88342; Cd68.
DR VEuPathDB; HostDB:ENSMUSG00000018774; -.
DR eggNOG; KOG4818; Eukaryota.
DR GeneTree; ENSGT00940000164775; -.
DR HOGENOM; CLU_071610_0_0_1; -.
DR InParanoid; P31996; -.
DR OrthoDB; 1321296at2759; -.
DR PhylomeDB; P31996; -.
DR TreeFam; TF316339; -.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 12514; 5 hits in 74 CRISPR screens.
DR PRO; PR:P31996; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; P31996; protein.
DR Bgee; ENSMUSG00000018774; Expressed in stroma of bone marrow and 176 other tissues.
DR ExpressionAtlas; P31996; baseline and differential.
DR Genevisible; P31996; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031902; C:late endosome membrane; IBA:GO_Central.
DR GO; GO:0005765; C:lysosomal membrane; IBA:GO_Central.
DR GO; GO:0005764; C:lysosome; IDA:ARUK-UCL.
DR GO; GO:0005886; C:plasma membrane; IDA:ARUK-UCL.
DR GO; GO:0007568; P:aging; IEP:ARUK-UCL.
DR GO; GO:0035425; P:autocrine signaling; ISO:MGI.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IDA:ARUK-UCL.
DR GO; GO:0031669; P:cellular response to nutrient levels; IDA:ARUK-UCL.
DR GO; GO:0071310; P:cellular response to organic substance; IDA:MGI.
DR GO; GO:0140052; P:cellular response to oxidised low-density lipoprotein particle stimulus; IDA:ARUK-UCL.
DR GO; GO:0072594; P:establishment of protein localization to organelle; IBA:GO_Central.
DR GO; GO:0002437; P:inflammatory response to antigenic stimulus; IDA:ARUK-UCL.
DR GO; GO:0002605; P:negative regulation of dendritic cell antigen processing and presentation; IMP:ARUK-UCL.
DR InterPro; IPR018134; LAMP_CS.
DR InterPro; IPR002000; Lysosome-assoc_membr_glycop.
DR PANTHER; PTHR11506; PTHR11506; 1.
DR Pfam; PF01299; Lamp; 1.
DR PRINTS; PR00336; LYSASSOCTDMP.
DR PROSITE; PS00311; LAMP_2; 1.
DR PROSITE; PS51407; LAMP_3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Disulfide bond; Endosome;
KW Glycoprotein; Lysosome; Membrane; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..326
FT /note="Macrosialin"
FT /id="PRO_0000017103"
FT TOPO_DOM 21..291
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..316
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00740"
FT TOPO_DOM 317..326
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00740"
FT REPEAT 44..49
FT /note="1-1"
FT REPEAT 50..64
FT /note="2-1"
FT REPEAT 65..72
FT /note="1-2"
FT REPEAT 73..88
FT /note="2-2"
FT REGION 21..109
FT /note="Mucin-like"
FT REGION 38..129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 108..122
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 60
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 84
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 97
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 129
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 134
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 169
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 218
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 233
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 251
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 139..177
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00740"
FT DISULFID 249..286
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00740"
FT VAR_SEQ 319..326
FT /note="Missing (in isoform Short)"
FT /evidence="ECO:0000303|PubMed:8486654"
FT /id="VSP_003043"
FT CONFLICT 117
FT /note="P -> T (in Ref. 3; AAC15685)"
FT /evidence="ECO:0000305"
FT CONFLICT 129
FT /note="N -> K (in Ref. 6; BAB21975)"
FT /evidence="ECO:0000305"
FT CONFLICT 137
FT /note="Q -> H (in Ref. 3; AAC15685)"
FT /evidence="ECO:0000305"
FT CONFLICT 205
FT /note="P -> T (in Ref. 6; BAB21975)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 326 AA; 34818 MW; AB7203A9A7EA47BA CRC64;
MRLPVCLILL GPLIAQGTEE DCPHKKAVTL LPSFTMTPTA TESTASPTTS HRPTTTSHGN
VTVHTSSGPT TVTHNPATTT SHGNATISHA TVSPTTNGTA TSPRSSTVGP HPGPPPPSPS
PRSKGALGNY TWANGSQPCV QLQAQIQIRI LYPIQGGRKA WGISVLNPNK TKVQGGCDGT
HPHLSLSFPY GQLTFGFKQD LHQSPSTVYL DYMAVEYNVS FPQAAQWTFM AQNSSLRELQ
APLGQSFCCG NASIVLSPAV HLDLLSLRLQ AAQLPDKGHF GPCFSCNRDQ SLLLPLIIGL
VLLGLLTLVL IAFCITRRRQ STYQPL
