CD82_HUMAN
ID CD82_HUMAN Reviewed; 267 AA.
AC P27701; D3DQN6; E9PC70; Q7Z2D4; Q7Z5N2;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=CD82 antigen;
DE AltName: Full=C33 antigen;
DE AltName: Full=IA4;
DE AltName: Full=Inducible membrane protein R2;
DE AltName: Full=Metastasis suppressor Kangai-1;
DE AltName: Full=Suppressor of tumorigenicity 6 protein;
DE AltName: Full=Tetraspanin-27;
DE Short=Tspan-27;
DE AltName: CD_antigen=CD82;
GN Name=CD82; Synonyms=KAI1, SAR2, ST6, TSPAN27;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Peripheral blood lymphocyte;
RX PubMed=1842498; DOI=10.1002/eji.1830210219;
RA Gaugitsch H.W., Hofer E., Huber N.E., Schnabl E., Baumruker T.;
RT "A new superfamily of lymphoid and melanoma cell proteins with extensive
RT homology to Schistosoma mansoni antigen Sm23.";
RL Eur. J. Immunol. 21:377-383(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=1401919;
RA Imai T., Fukudome K., Takagi S., Nagira M., Furuse M., Fukuhara N.,
RA Nishimura M., Hinuma Y., Yoshie O.;
RT "C33 antigen recognized by monoclonal antibodies inhibitory to human T cell
RT leukemia virus type 1-induced syncytium formation is a member of a new
RT family of transmembrane proteins including CD9, CD37, CD53, and CD63.";
RL J. Immunol. 149:2879-2886(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=7754374; DOI=10.1126/science.7754374;
RA Dong J.T., Barrett J.C.;
RT "KAI1, a metastasis suppressor gene for prostate cancer on human chromosome
RT 11p11.2.";
RL Science 268:884-886(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND VARIANT VAL-241.
RA Zhou G., Li S., Li H., Shen C., Li M., Xiao W., Lin L., Yang S.;
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9126478; DOI=10.1006/geno.1997.4618;
RA Dong J.T., Isaacs W.B., Barrett J.C., Isaacs J.T.;
RT "Genomic organization of the human KAI1 metastasis-suppressor gene.";
RL Genomics 41:25-32(1997).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P.,
RA Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y.,
RA LaBaer J.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT VAL-241.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT VAL-241.
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [10]
RP INTERACTION WITH IGSF8.
RX PubMed=12750295;
RA Zhang X.A., Lane W.S., Charrin S., Rubinstein E., Liu L.;
RT "EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits
RT the migration of prostate cancer cells.";
RL Cancer Res. 63:2665-2674(2003).
RN [11]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129.
RC TISSUE=Liver;
RX PubMed=19159218; DOI=10.1021/pr8008012;
RA Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT "Glycoproteomics analysis of human liver tissue by combination of multiple
RT enzyme digestion and hydrazide chemistry.";
RL J. Proteome Res. 8:651-661(2009).
RN [12]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129 AND ASN-198.
RC TISSUE=Leukemic T-cell;
RX PubMed=19349973; DOI=10.1038/nbt.1532;
RA Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M.,
RA Schiess R., Aebersold R., Watts J.D.;
RT "Mass-spectrometric identification and relative quantification of N-linked
RT cell surface glycoproteins.";
RL Nat. Biotechnol. 27:378-386(2009).
RN [13]
RP SUBCELLULAR LOCATION.
RX PubMed=30463011; DOI=10.1016/j.celrep.2018.10.088;
RA Shah J., Rouaud F., Guerrera D., Vasileva E., Popov L.M., Kelley W.L.,
RA Rubinstein E., Carette J.E., Amieva M.R., Citi S.;
RT "A Dock-and-Lock Mechanism Clusters ADAM10 at Cell-Cell Junctions to
RT Promote alpha-Toxin Cytotoxicity.";
RL Cell Rep. 25:2132-2147(2018).
CC -!- FUNCTION: Associates with CD4 or CD8 and delivers costimulatory signals
CC for the TCR/CD3 pathway.
CC -!- SUBUNIT: Interacts directly with IGSF8. {ECO:0000269|PubMed:12750295}.
CC -!- INTERACTION:
CC P27701; O15552: FFAR2; NbExp=3; IntAct=EBI-682379, EBI-2833872;
CC P27701; P26715: KLRC1; NbExp=3; IntAct=EBI-682379, EBI-9018187;
CC P27701; Q9BZL3: SMIM3; NbExp=3; IntAct=EBI-682379, EBI-741850;
CC P27701; P01033: TIMP1; NbExp=11; IntAct=EBI-682379, EBI-712536;
CC P27701; Q8TAA9: VANGL1; NbExp=6; IntAct=EBI-682379, EBI-682393;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:30463011};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P27701-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P27701-2; Sequence=VSP_045656;
CC -!- TISSUE SPECIFICITY: Lymphoid specific.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC Haematology;
CC URL="http://atlasgeneticsoncology.org/Genes/CD82ID41045ch11p11.html";
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DR EMBL; X53795; CAA37804.1; -; mRNA.
DR EMBL; S48196; AAB23825.1; -; mRNA.
DR EMBL; U20770; AAC50133.1; -; mRNA.
DR EMBL; AY303776; AAP76181.1; -; mRNA.
DR EMBL; U67274; AAC51205.1; -; Genomic_DNA.
DR EMBL; U67268; AAC51205.1; JOINED; Genomic_DNA.
DR EMBL; U67269; AAC51205.1; JOINED; Genomic_DNA.
DR EMBL; U67270; AAC51205.1; JOINED; Genomic_DNA.
DR EMBL; U67271; AAC51205.1; JOINED; Genomic_DNA.
DR EMBL; U67272; AAC51205.1; JOINED; Genomic_DNA.
DR EMBL; U67273; AAC51205.1; JOINED; Genomic_DNA.
DR EMBL; CR542255; CAG47051.1; -; mRNA.
DR EMBL; AC010768; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471064; EAW68063.1; -; Genomic_DNA.
DR EMBL; CH471064; EAW68064.1; -; Genomic_DNA.
DR EMBL; BC000726; AAH00726.1; -; mRNA.
DR EMBL; BC001821; AAH01821.1; -; mRNA.
DR CCDS; CCDS31469.1; -. [P27701-2]
DR CCDS; CCDS7909.1; -. [P27701-1]
DR PIR; I38942; A46493.
DR RefSeq; NP_001020015.1; NM_001024844.1. [P27701-2]
DR RefSeq; NP_002222.1; NM_002231.3. [P27701-1]
DR RefSeq; XP_006718286.1; XM_006718223.2. [P27701-1]
DR AlphaFoldDB; P27701; -.
DR SMR; P27701; -.
DR BioGRID; 109935; 38.
DR IntAct; P27701; 20.
DR MINT; P27701; -.
DR STRING; 9606.ENSP00000227155; -.
DR GlyConnect; 1089; 2 N-Linked glycans (1 site).
DR GlyGen; P27701; 3 sites, 2 N-linked glycans (1 site).
DR iPTMnet; P27701; -.
DR PhosphoSitePlus; P27701; -.
DR SwissPalm; P27701; -.
DR BioMuta; CD82; -.
DR DMDM; 131775; -.
DR EPD; P27701; -.
DR jPOST; P27701; -.
DR MassIVE; P27701; -.
DR MaxQB; P27701; -.
DR PaxDb; P27701; -.
DR PeptideAtlas; P27701; -.
DR PRIDE; P27701; -.
DR ProteomicsDB; 19382; -.
DR ProteomicsDB; 54407; -. [P27701-1]
DR Antibodypedia; 3743; 654 antibodies from 43 providers.
DR DNASU; 3732; -.
DR Ensembl; ENST00000227155.9; ENSP00000227155.4; ENSG00000085117.12. [P27701-1]
DR Ensembl; ENST00000342935.7; ENSP00000339686.3; ENSG00000085117.12. [P27701-2]
DR GeneID; 3732; -.
DR KEGG; hsa:3732; -.
DR MANE-Select; ENST00000227155.9; ENSP00000227155.4; NM_002231.4; NP_002222.1.
DR UCSC; uc001myc.4; human. [P27701-1]
DR CTD; 3732; -.
DR DisGeNET; 3732; -.
DR GeneCards; CD82; -.
DR HGNC; HGNC:6210; CD82.
DR HPA; ENSG00000085117; Low tissue specificity.
DR MIM; 600623; gene.
DR neXtProt; NX_P27701; -.
DR OpenTargets; ENSG00000085117; -.
DR PharmGKB; PA142672155; -.
DR VEuPathDB; HostDB:ENSG00000085117; -.
DR eggNOG; KOG3882; Eukaryota.
DR GeneTree; ENSGT00940000158481; -.
DR InParanoid; P27701; -.
DR OMA; NIHSEDY; -.
DR OrthoDB; 1207549at2759; -.
DR PhylomeDB; P27701; -.
DR TreeFam; TF352892; -.
DR PathwayCommons; P27701; -.
DR SignaLink; P27701; -.
DR SIGNOR; P27701; -.
DR BioGRID-ORCS; 3732; 7 hits in 1078 CRISPR screens.
DR ChiTaRS; CD82; human.
DR GeneWiki; CD82_(gene); -.
DR GenomeRNAi; 3732; -.
DR Pharos; P27701; Tbio.
DR PRO; PR:P27701; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; P27701; protein.
DR Bgee; ENSG00000085117; Expressed in olfactory bulb and 192 other tissues.
DR ExpressionAtlas; P27701; baseline and differential.
DR Genevisible; P27701; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR Gene3D; 1.10.1450.10; -; 1.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR InterPro; IPR000301; Tetraspanin_animals.
DR InterPro; IPR018503; Tetraspanin_CS.
DR InterPro; IPR008952; Tetraspanin_EC2_sf.
DR PANTHER; PTHR19282; PTHR19282; 1.
DR Pfam; PF00335; Tetraspanin; 1.
DR PIRSF; PIRSF002419; Tetraspanin; 1.
DR SUPFAM; SSF48652; SSF48652; 1.
DR PROSITE; PS00421; TM4_1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..267
FT /note="CD82 antigen"
FT /id="PRO_0000219226"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..53
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..83
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..228
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..267
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 129
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19159218,
FT ECO:0000269|PubMed:19349973"
FT CARBOHYD 157
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 198
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19349973"
FT VAR_SEQ 88..112
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_045656"
FT VARIANT 241
FT /note="I -> V (in dbSNP:rs1139971)"
FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.4,
FT ECO:0000269|Ref.8"
FT /id="VAR_052326"
FT CONFLICT 124
FT /note="L -> P (in Ref. 4; AAP76181)"
FT /evidence="ECO:0000305"
FT CONFLICT 240..241
FT /note="II -> MV (in Ref. 2; AAB23825)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 267 AA; 29626 MW; FC379855BD1CABDE CRC64;
MGSACIKVTK YFLFLFNLIF FILGAVILGF GVWILADKSS FISVLQTSSS SLRMGAYVFI
GVGAVTMLMG FLGCIGAVNE VRCLLGLYFA FLLLILIAQV TAGALFYFNM GKLKQEMGGI
VTELIRDYNS SREDSLQDAW DYVQAQVKCC GWVSFYNWTD NAELMNRPEV TYPCSCEVKG
EEDNSLSVRK GFCEAPGNRT QSGNHPEDWP VYQEGCMEKV QAWLQENLGI ILGVGVGVAI
IELLGMVLSI CLCRHVHSED YSKVPKY
