CDC21_ARATH
ID CDC21_ARATH Reviewed; 457 AA.
AC Q9SZA4; Q8GY38; Q9SQK2;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Cell division cycle 20.1, cofactor of APC complex;
DE Short=AtCDC20.1;
GN Name=CDC20-1; Synonyms=CDC20_2; OrderedLocusNames=At4g33270;
GN ORFNames=F17M5.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Arioli T.;
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP REVIEW.
RX PubMed=12597875; DOI=10.1016/s1360-1385(02)00028-6;
RA Capron A., Okresz L., Genschik P.;
RT "First glance at the plant APC/C, a highly conserved ubiquitin-protein
RT ligase.";
RL Trends Plant Sci. 8:83-89(2003).
RN [7]
RP REVIEW, AND GENE FAMILY.
RX PubMed=21087491; DOI=10.1186/1471-2229-10-254;
RA Lima M.D.F., Eloy N.B., Pegoraro C., Sagit R., Rojas C., Bretz T.,
RA Vargas L., Elofsson A., de Oliveira A.C., Hemerly A.S., Ferreira P.C.G.;
RT "Genomic evolution and complexity of the Anaphase-promoting Complex (APC)
RT in land plants.";
RL BMC Plant Biol. 10:254-254(2010).
RN [8]
RP INTERACTION WITH GIG1 AND PYM.
RX PubMed=22167058; DOI=10.1105/tpc.111.092049;
RA Iwata E., Ikeda S., Matsunaga S., Kurata M., Yoshioka Y., Criqui M.-C.,
RA Genschik P., Ito M.;
RT "GIGAS CELL1, a novel negative regulator of the anaphase-promoting
RT complex/cyclosome, is required for proper mitotic progression and cell fate
RT determination in Arabidopsis.";
RL Plant Cell 23:4382-4393(2011).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP INTERACTION WITH CYCA1-2; CYCB2-1; CYCB2-2; APC10; FZR1; FZR2; FZR3; MAD2;
RP BUB3.1; BUBR1 AND BUB1, AND INDUCTION.
RX PubMed=21687678; DOI=10.1371/journal.pone.0020618;
RA Kevei Z., Baloban M., Da Ines O., Tiricz H., Kroll A., Regulski K.,
RA Mergaert P., Kondorosi E.;
RT "Conserved CDC20 cell cycle functions are carried out by two of the five
RT isoforms in Arabidopsis thaliana.";
RL PLoS ONE 6:E20618-E20618(2011).
RN [10]
RP INTERACTION WITH GIG1.
RX PubMed=22844260; DOI=10.1371/journal.pgen.1002865;
RA Cromer L., Heyman J., Touati S., Harashima H., Araou E., Girard C.,
RA Horlow C., Wassmann K., Schnittger A., De Veylder L., Mercier R.;
RT "OSD1 promotes meiotic progression via APC/C inhibition and forms a
RT regulatory network with TDM and CYCA1;2/TAM.";
RL PLoS Genet. 8:E1002865-E1002865(2012).
RN [11]
RP INTERACTION WITH PANS1.
RX PubMed=24206843; DOI=10.1016/j.cub.2013.08.036;
RA Cromer L., Jolivet S., Horlow C., Chelysheva L., Heyman J., De Jaeger G.,
RA Koncz C., De Veylder L., Mercier R.;
RT "Centromeric cohesion is protected twice at meiosis, by SHUGOSHINs at
RT anaphase I and by PATRONUS at interkinesis.";
RL Curr. Biol. 23:2090-2099(2013).
CC -!- FUNCTION: Component of the anaphase promoting complex/cyclosome
CC (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex
CC that controls progression through mitosis and the G1 phase of the cell
CC cycle. {ECO:0000250, ECO:0000269|PubMed:21687678}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: The APC/C is composed of at least 11 subunits that stay
CC tightly associated throughout the cell cycle (By similarity). Interacts
CC with APC10, FZR1, FZR2, FZR3 (PubMed:21687678). Binds to GIG1 and PYM
CC (PubMed:22167058, PubMed:22844260). Part of the mitotic checkpoint
CC complex (MCC); interacts with MAD2, BUB3.1, BUBR1 and BUB1
CC (PubMed:21687678). Binds to cyclins CYCA1-2, CYCB2-1 and CYCB2-2
CC (PubMed:21687678). Interacts with PANS1 (PubMed:24206843).
CC {ECO:0000250, ECO:0000269|PubMed:21687678, ECO:0000269|PubMed:22167058,
CC ECO:0000269|PubMed:22844260, ECO:0000269|PubMed:24206843}.
CC -!- INTERACTION:
CC Q9SZA4; Q8LGU6: CDC27B; NbExp=2; IntAct=EBI-1668707, EBI-1668733;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21687678}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SZA4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SZA4-2; Sequence=VSP_047672;
CC -!- TISSUE SPECIFICITY: Expressed in meristems and organ primordia. Present
CC in flowers, leaves, stems, roots, pollen grains and developing seeds.
CC {ECO:0000269|PubMed:21687678}.
CC -!- INDUCTION: Expression starts to rise in the S-phase and peaks in the M-
CC phase with a non-negligible basic expression level also in the other
CC cell cycle phases. {ECO:0000269|PubMed:21687678}.
CC -!- DISRUPTION PHENOTYPE: Delay in plant development due to reduced cell
CC number and male sterility. {ECO:0000269|PubMed:21687678}.
CC -!- SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Arabidopsis APC/C subunits;
CC URL="http://personal.rhul.ac.uk/ujba/110/apc/APC.htm";
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DR EMBL; AF029262; AAF14048.1; -; mRNA.
DR EMBL; AL035678; CAB38785.1; -; Genomic_DNA.
DR EMBL; AL161583; CAB80044.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86200.1; -; Genomic_DNA.
DR EMBL; AK117888; BAC42527.1; -; mRNA.
DR EMBL; BT026138; ABG48494.1; -; mRNA.
DR PIR; T05978; T05978.
DR RefSeq; NP_195053.1; NM_119481.3. [Q9SZA4-1]
DR AlphaFoldDB; Q9SZA4; -.
DR SMR; Q9SZA4; -.
DR BioGRID; 14748; 22.
DR ELM; Q9SZA4; -.
DR IntAct; Q9SZA4; 14.
DR STRING; 3702.AT4G33270.1; -.
DR PaxDb; Q9SZA4; -.
DR PRIDE; Q9SZA4; -.
DR ProteomicsDB; 220464; -. [Q9SZA4-1]
DR EnsemblPlants; AT4G33270.1; AT4G33270.1; AT4G33270. [Q9SZA4-1]
DR GeneID; 829463; -.
DR Gramene; AT4G33270.1; AT4G33270.1; AT4G33270. [Q9SZA4-1]
DR KEGG; ath:AT4G33270; -.
DR Araport; AT4G33270; -.
DR TAIR; locus:2119151; AT4G33270.
DR eggNOG; KOG0305; Eukaryota.
DR HOGENOM; CLU_014831_6_1_1; -.
DR InParanoid; Q9SZA4; -.
DR OMA; NGHEDRV; -.
DR OrthoDB; 1220675at2759; -.
DR PhylomeDB; Q9SZA4; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9SZA4; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SZA4; baseline and differential.
DR Genevisible; Q9SZA4; AT.
DR GO; GO:0005680; C:anaphase-promoting complex; IBA:GO_Central.
DR GO; GO:0033597; C:mitotic checkpoint complex; IPI:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0010997; F:anaphase-promoting complex binding; IBA:GO_Central.
DR GO; GO:0019900; F:kinase binding; IPI:UniProtKB.
DR GO; GO:1990757; F:ubiquitin ligase activator activity; IBA:GO_Central.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:1905786; P:positive regulation of anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR024977; Apc4_WD40_dom.
DR InterPro; IPR033010; Cdc20/Fizzy.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19918; PTHR19918; 1.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF00400; WD40; 3.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Mitosis; Nucleus;
KW Reference proteome; Repeat; Ubl conjugation pathway; WD repeat.
FT CHAIN 1..457
FT /note="Cell division cycle 20.1, cofactor of APC complex"
FT /id="PRO_0000423306"
FT REPEAT 138..175
FT /note="WD 1"
FT REPEAT 180..219
FT /note="WD 2"
FT REPEAT 223..260
FT /note="WD 3"
FT REPEAT 264..303
FT /note="WD 4"
FT REPEAT 312..354
FT /note="WD 5"
FT REPEAT 356..397
FT /note="WD 6"
FT REPEAT 400..439
FT /note="WD 7"
FT VAR_SEQ 1..242
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074"
FT /id="VSP_047672"
FT CONFLICT 441..457
FT /note="AAPKAVSEPFSHVNRIR -> SCSKSSFRAIFSRESYSLKL (in Ref.
FT 1; AAF14048)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 457 AA; 50551 MW; C6D71FC311998A9E CRC64;
MDAGMNNTSS HYKTQARCPL QEHFLPRKPS KENLDRFIPN RSAMNFDYAH FALTEGRKGK
DQTAAVSSPS KEAYRKQLAE TMNLNHTRIL AFRNKPQAPV ELLPSNHSAS LHQQPKSVKP
RRYIPQTSER TLDAPDIVDD FYLNLLDWGS ANVLAIALDH TVYLWDASTG STSELVTIDE
EKGPVTSINW APDGRHVAVG LNNSEVQLWD SASNRQLRTL KGGHQSRVGS LAWNNHILTT
GGMDGLIINN DVRIRSPIVE TYRGHTQEVC GLKWSGSGQQ LASGGNDNVV HIWDRSVASS
NSTTQWLHRL EEHTSAVKAL AWCPFQANLL ATGGGGGDRT IKFWNTHTGA CLNSVDTGSQ
VCSLLWSKNE RELLSSHGFT QNQLTLWKYP SMVKMAELTG HTSRVLYMAQ SPDGCTVASA
AGDETLRFWN VFGVPETAKK AAPKAVSEPF SHVNRIR
