CDC22_ARATH
ID CDC22_ARATH Reviewed; 447 AA.
AC Q9S7I8; F4JW04;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Cell division cycle 20.2, cofactor of APC complex;
DE Short=AtCDC20.2;
GN Name=CDC20-2; Synonyms=CDC20_1; OrderedLocusNames=At4g33260;
GN ORFNames=F17M5.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Arioli T.;
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP REVIEW.
RX PubMed=12597875; DOI=10.1016/s1360-1385(02)00028-6;
RA Capron A., Okresz L., Genschik P.;
RT "First glance at the plant APC/C, a highly conserved ubiquitin-protein
RT ligase.";
RL Trends Plant Sci. 8:83-89(2003).
RN [5]
RP REVIEW, AND GENE FAMILY.
RX PubMed=21087491; DOI=10.1186/1471-2229-10-254;
RA Lima M.D.F., Eloy N.B., Pegoraro C., Sagit R., Rojas C., Bretz T.,
RA Vargas L., Elofsson A., de Oliveira A.C., Hemerly A.S., Ferreira P.C.G.;
RT "Genomic evolution and complexity of the Anaphase-promoting Complex (APC)
RT in land plants.";
RL BMC Plant Biol. 10:254-254(2010).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP INTERACTION WITH CYCA1-2; CYCB2-1; CYCB2-2; APC10; FZR1; FZR2; FZR3; MAD2;
RP BUB3.1; BUBR1 AND BUB1, AND INDUCTION.
RX PubMed=21687678; DOI=10.1371/journal.pone.0020618;
RA Kevei Z., Baloban M., Da Ines O., Tiricz H., Kroll A., Regulski K.,
RA Mergaert P., Kondorosi E.;
RT "Conserved CDC20 cell cycle functions are carried out by two of the five
RT isoforms in Arabidopsis thaliana.";
RL PLoS ONE 6:E20618-E20618(2011).
CC -!- FUNCTION: Component of the anaphase promoting complex/cyclosome
CC (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex
CC that controls progression through mitosis and the G1 phase of the cell
CC cycle. {ECO:0000250, ECO:0000269|PubMed:21687678}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: The APC/C is composed of at least 11 subunits that stay
CC tightly associated throughout the cell cycle (By similarity). Interacts
CC with APC10, FZR1, FZR2, FZR3. Part of the mitotic checkpoint complex
CC (MCC); interacts with MAD2, BUB3.1, BUBR1 and BUB1. Binds to cyclins
CC CYCA1-2, CYCB2-1 and CYCB2-2. {ECO:0000250,
CC ECO:0000269|PubMed:21687678}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21687678}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9S7I8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9S7I8-2; Sequence=VSP_047673;
CC -!- TISSUE SPECIFICITY: Expressed in meristems and organ primordia. Present
CC in flowers, leaves, stems and roots. {ECO:0000269|PubMed:21687678}.
CC -!- INDUCTION: Expression starts to rise in the S-phase and peaks in the M-
CC phase with a non-negligible basic expression level also in the other
CC cell cycle phases. {ECO:0000269|PubMed:21687678}.
CC -!- DISRUPTION PHENOTYPE: Delay in plant development due to reduced cell
CC number and male sterility. {ECO:0000269|PubMed:21687678}.
CC -!- SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Arabidopsis APC/C subunits;
CC URL="http://personal.rhul.ac.uk/ujba/110/apc/APC.htm";
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF029263; AAF14049.1; -; mRNA.
DR EMBL; AL035678; CAB38784.1; -; Genomic_DNA.
DR EMBL; AL161583; CAB80043.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86198.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86199.1; -; Genomic_DNA.
DR PIR; T05977; T05977.
DR RefSeq; NP_001190900.1; NM_001203971.1. [Q9S7I8-2]
DR RefSeq; NP_195052.1; NM_119480.4. [Q9S7I8-1]
DR AlphaFoldDB; Q9S7I8; -.
DR SMR; Q9S7I8; -.
DR BioGRID; 14747; 7.
DR ELM; Q9S7I8; -.
DR IntAct; Q9S7I8; 1.
DR STRING; 3702.AT4G33260.1; -.
DR MetOSite; Q9S7I8; -.
DR PaxDb; Q9S7I8; -.
DR PRIDE; Q9S7I8; -.
DR EnsemblPlants; AT4G33260.1; AT4G33260.1; AT4G33260. [Q9S7I8-1]
DR EnsemblPlants; AT4G33260.2; AT4G33260.2; AT4G33260. [Q9S7I8-2]
DR GeneID; 829462; -.
DR Gramene; AT4G33260.1; AT4G33260.1; AT4G33260. [Q9S7I8-1]
DR Gramene; AT4G33260.2; AT4G33260.2; AT4G33260. [Q9S7I8-2]
DR KEGG; ath:AT4G33260; -.
DR Araport; AT4G33260; -.
DR TAIR; locus:2119141; AT4G33260.
DR eggNOG; KOG0305; Eukaryota.
DR HOGENOM; CLU_014831_6_1_1; -.
DR InParanoid; Q9S7I8; -.
DR OMA; CMSTDGE; -.
DR OrthoDB; 1220675at2759; -.
DR PhylomeDB; Q9S7I8; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9S7I8; -.
DR Proteomes; UP000006548; Chromosome 4.
DR Genevisible; Q9S7I8; AT.
DR GO; GO:0005680; C:anaphase-promoting complex; IBA:GO_Central.
DR GO; GO:0033597; C:mitotic checkpoint complex; IPI:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0010997; F:anaphase-promoting complex binding; IBA:GO_Central.
DR GO; GO:0019900; F:kinase binding; IPI:UniProtKB.
DR GO; GO:1990757; F:ubiquitin ligase activator activity; IBA:GO_Central.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:1905786; P:positive regulation of anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR024977; Apc4_WD40_dom.
DR InterPro; IPR033010; Cdc20/Fizzy.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19918; PTHR19918; 1.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF00400; WD40; 3.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Mitosis; Nucleus;
KW Reference proteome; Repeat; Ubl conjugation pathway; WD repeat.
FT CHAIN 1..447
FT /note="Cell division cycle 20.2, cofactor of APC complex"
FT /id="PRO_0000423307"
FT REPEAT 128..165
FT /note="WD 1"
FT REPEAT 170..209
FT /note="WD 2"
FT REPEAT 213..250
FT /note="WD 3"
FT REPEAT 254..293
FT /note="WD 4"
FT REPEAT 302..344
FT /note="WD 5"
FT REPEAT 346..387
FT /note="WD 6"
FT REPEAT 390..429
FT /note="WD 7"
FT VAR_SEQ 417..447
FT /note="RFWNVFGVPETAKKAAPKAVAEPFSHVNRIR -> SCRAIFSCESYSMKLLK
FT TKTTRKTH (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_047673"
SQ SEQUENCE 447 AA; 49504 MW; 863312116D03B7AD CRC64;
MDAGLNRCPL QEHFLPRKNS KENLDRFIPN RSAMNFDYAH FALTEERKGK DQSATVSSPS
KEAYRKQLAE TMNLNHTRIL AFRNKPQAPV ELLPSNHSAS LHQQPKSVKP RRYIPQTSER
TLDAPDIVDD FYLNLLDWGS ANVLAIALDH TVYLWDASTG STSELVTIDE EKGPVTSINW
APDGRHVAVG LNNSEVQLWD SASNRQLRTL KGGHQSRVGS LAWNNHILTT GGMDGLIINN
DVRIRSPIVE TYRGHTQEVC GLKWSGSGQQ LASGGNDNVV HIWDRSVASS NSTTQWLHRL
EEHTSAVKAL AWCPFQANLL ATGGGGGDRT IKFWNTHTGA CLNSVDTGSQ VCSLLWSKNE
RELLSSHGFT QNQLTLWKYP SMVKMAELTG HTSRVLYMAQ SPDGCTVASA AGDETLRFWN
VFGVPETAKK AAPKAVAEPF SHVNRIR
