CDC23_CAEEL
ID CDC23_CAEEL Reviewed; 673 AA.
AC Q19294;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Cell division cycle protein 23 homolog {ECO:0000303|PubMed:11134076};
DE AltName: Full=Anaphase-promoting complex subunit 8 {ECO:0000250|UniProtKB:Q9UJX2};
DE Short=APC8 {ECO:0000250|UniProtKB:Q9UJX2};
DE AltName: Full=Metaphase-to-anaphase transition defect protein 3 {ECO:0000312|WormBase:F10C5.1};
GN Name=mat-3 {ECO:0000312|WormBase:F10C5.1};
GN Synonyms=apc-8 {ECO:0000312|WormBase:F10C5.1},
GN cdc-23 {ECO:0000312|WormBase:F10C5.1},
GN pod-4 {ECO:0000312|WormBase:F10C5.1}, vex-2 {ECO:0000312|WormBase:F10C5.1};
GN ORFNames=F10C5.1 {ECO:0000312|WormBase:F10C5.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF SER-502.
RX PubMed=11134076; DOI=10.1083/jcb.151.7.1469;
RA Golden A., Sadler P.L., Wallenfang M.R., Schumacher J.M., Hamill D.R.,
RA Bates G., Bowerman B., Seydoux G., Shakes D.C.;
RT "Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis
RT elegans.";
RL J. Cell Biol. 151:1469-1482(2000).
RN [3] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11832245; DOI=10.1016/s1534-5807(02)00114-4;
RA Rappleye C.A., Tagawa A., Lyczak R., Bowerman B., Aroian R.V.;
RT "The anaphase-promoting complex and separin are required for embryonic
RT anterior-posterior axis formation.";
RL Dev. Cell 2:195-206(2002).
RN [4] {ECO:0000305}
RP FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF GLU-70; GLU-161;
RP GLU-237; ALA-339; GLU-401; ALA-451; SER-502; ALA-528 AND GLY-532.
RX PubMed=11861581; DOI=10.1093/genetics/160.2.805;
RA Davis E.S., Wille L., Chestnut B.A., Sadler P.L., Shakes D.C., Golden A.;
RT "Multiple subunits of the Caenorhabditis elegans anaphase-promoting complex
RT are required for chromosome segregation during meiosis I.";
RL Genetics 160:805-813(2002).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=15556870; DOI=10.1016/j.cub.2004.11.010;
RA Juo P., Kaplan J.M.;
RT "The anaphase-promoting complex regulates the abundance of GLR-1 glutamate
RT receptors in the ventral nerve cord of C. elegans.";
RL Curr. Biol. 14:2057-2062(2004).
RN [6] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15238519; DOI=10.1534/genetics.103.026021;
RA Garbe D., Doto J.B., Sundaram M.V.;
RT "Caenorhabditis elegans lin-35/Rb, efl-1/E2F and other synthetic multivulva
RT genes negatively regulate the anaphase-promoting complex gene mat-3/APC8.";
RL Genetics 167:663-672(2004).
RN [7] {ECO:0000305}
RP MUTAGENESIS OF LEU-433 AND SER-502.
RX PubMed=17057243; DOI=10.1534/genetics.106.059105;
RA Stein K.K., Davis E.S., Hays T., Golden A.;
RT "Components of the spindle assembly checkpoint regulate the anaphase-
RT promoting complex during meiosis in Caenorhabditis elegans.";
RL Genetics 175:107-123(2007).
RN [8] {ECO:0000305}
RP FUNCTION.
RX PubMed=24321454; DOI=10.1016/j.mcn.2013.12.001;
RA Kowalski J.R., Dube H., Touroutine D., Rush K.M., Goodwin P.R., Carozza M.,
RA Didier Z., Francis M.M., Juo P.;
RT "The anaphase-promoting complex (APC) ubiquitin ligase regulates GABA
RT transmission at the C. elegans neuromuscular junction.";
RL Mol. Cell. Neurosci. 58:62-75(2014).
CC -!- FUNCTION: Probable component of the anaphase promoting
CC complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase
CC that controls progression through mitosis and the G1 phase of the cell
CC cycle (PubMed:11861581, PubMed:15238519). The APC/C complex acts by
CC mediating ubiquitination and subsequent degradation of target proteins
CC (PubMed:11861581). Developmental role in early embryogenesis and the
CC metaphase to anaphase transition in oocyte and spermatocyte meiosis and
CC mitosis in germ cells (PubMed:11134076, PubMed:11861581,
CC PubMed:15238519). Required for embryonic anterior-posterior axis
CC formation (PubMed:11832245). Plays a role in regulating the abundance
CC of glr-1 receptors in postmitotic neurons, which may in turn control
CC animal locomotion (PubMed:15556870). Involved in regulating GABA
CC neurotransmitter release at neuromuscular junctions in GABA motor
CC neurons (PubMed:24321454). {ECO:0000269|PubMed:11134076,
CC ECO:0000269|PubMed:11832245, ECO:0000269|PubMed:11861581,
CC ECO:0000269|PubMed:15238519, ECO:0000269|PubMed:15556870,
CC ECO:0000269|PubMed:24321454}.
CC -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC -!- SUBUNIT: The APC/C complex is probably composed of at least 12
CC subunits: apc-2, apc-10, apc-11, cdc-26, emb-1, emb-27, emb-30, mat-1,
CC mat-2, mat-3, such-1 and gfi-3. {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Developmental defects in the gonad, germline,
CC male tail and hermaphrodite vulva (PubMed:15238519). Increased number
CC of germ cells arrested at the metaphase stage of mitosis during the
CC larval stage L4; by adulthood, there are no nuclei in the distal part
CC of the gonad and as a result, no sperm or oocytes are formed
CC (PubMed:15238519). RNAi-mediated knockdown results in defective
CC metaphase to anaphase transition (Mat phenotype) and embryos that
CC arrest at the one-cell stage that display defects in the formation of
CC the anterior-posterior axis (PubMed:11861581, PubMed:11832245).
CC {ECO:0000269|PubMed:11832245, ECO:0000269|PubMed:11861581,
CC ECO:0000269|PubMed:15238519}.
CC -!- SIMILARITY: Belongs to the APC8/CDC23 family. {ECO:0000305}.
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DR EMBL; FO081107; CCD69151.1; -; Genomic_DNA.
DR RefSeq; NP_497203.1; NM_064802.4.
DR AlphaFoldDB; Q19294; -.
DR SMR; Q19294; -.
DR ComplexPortal; CPX-3382; Anaphase Promoting Complex/Cyclosome.
DR DIP; DIP-24631N; -.
DR IntAct; Q19294; 2.
DR STRING; 6239.F10C5.1; -.
DR EPD; Q19294; -.
DR PaxDb; Q19294; -.
DR PeptideAtlas; Q19294; -.
DR EnsemblMetazoa; F10C5.1.1; F10C5.1.1; WBGene00003134.
DR GeneID; 175205; -.
DR KEGG; cel:CELE_F10C5.1; -.
DR UCSC; F10C5.1; c. elegans.
DR CTD; 175205; -.
DR WormBase; F10C5.1; CE26888; WBGene00003134; mat-3.
DR eggNOG; KOG1155; Eukaryota.
DR GeneTree; ENSGT00950000182950; -.
DR HOGENOM; CLU_018320_3_0_1; -.
DR InParanoid; Q19294; -.
DR OMA; MWTNEIL; -.
DR OrthoDB; 761109at2759; -.
DR PhylomeDB; Q19294; -.
DR Reactome; R-CEL-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR SignaLink; Q19294; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q19294; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00003134; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005680; C:anaphase-promoting complex; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0008356; P:asymmetric cell division; IMP:UniProtKB.
DR GO; GO:0051301; P:cell division; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0007091; P:metaphase/anaphase transition of mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR GO; GO:0009949; P:polarity specification of anterior/posterior axis; IMP:UniProtKB.
DR GO; GO:0045842; P:positive regulation of mitotic metaphase/anaphase transition; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR GO; GO:0051445; P:regulation of meiotic cell cycle; IC:ComplexPortal.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; IC:ComplexPortal.
DR GO; GO:1904666; P:regulation of ubiquitin protein ligase activity; IC:ComplexPortal.
DR Gene3D; 1.25.40.10; -; 2.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF13181; TPR_8; 2.
DR SMART; SM00028; TPR; 7.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS50005; TPR; 5.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Developmental protein; Meiosis; Mitosis;
KW Neurotransmitter transport; Reference proteome; Repeat; TPR repeat;
KW Transport; Ubl conjugation pathway.
FT CHAIN 1..673
FT /note="Cell division cycle protein 23 homolog"
FT /evidence="ECO:0000305"
FT /id="PRO_0000435330"
FT REPEAT 86..120
FT /note="TPR 1"
FT /evidence="ECO:0000255"
FT REPEAT 159..195
FT /note="TPR 2"
FT /evidence="ECO:0000255"
FT REPEAT 232..267
FT /note="TPR 3"
FT /evidence="ECO:0000255"
FT REPEAT 332..365
FT /note="TPR 4"
FT /evidence="ECO:0000255"
FT REPEAT 400..433
FT /note="TPR 5"
FT /evidence="ECO:0000255"
FT REPEAT 434..467
FT /note="TPR 6"
FT /evidence="ECO:0000255"
FT REPEAT 469..501
FT /note="TPR 7"
FT /evidence="ECO:0000255"
FT REPEAT 502..535
FT /note="TPR 8"
FT /evidence="ECO:0000255"
FT REPEAT 539..572
FT /note="TPR 9"
FT /evidence="ECO:0000255"
FT REPEAT 577..610
FT /note="TPR 10"
FT /evidence="ECO:0000255"
FT REGION 628..673
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 654..673
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 70
FT /note="E->K: In or192; results in sterility."
FT /evidence="ECO:0000269|PubMed:11861581"
FT MUTAGEN 161
FT /note="E->K: In ax68; results in sterility."
FT /evidence="ECO:0000269|PubMed:11861581"
FT MUTAGEN 237
FT /note="E->K: In ax82; results in sterility."
FT /evidence="ECO:0000269|PubMed:11861581"
FT MUTAGEN 339
FT /note="A->L: In ax77; results in sterility."
FT /evidence="ECO:0000269|PubMed:11861581"
FT MUTAGEN 401
FT /note="E->K: In ax79 and ax136; results in sterility."
FT /evidence="ECO:0000269|PubMed:11861581"
FT MUTAGEN 433
FT /note="L->F: In av26; weak suppressor of mat-3 mutations."
FT /evidence="ECO:0000269|PubMed:17057243"
FT MUTAGEN 451
FT /note="A->V: In ax70; results in sterility."
FT /evidence="ECO:0000269|PubMed:11861581"
FT MUTAGEN 502
FT /note="S->F: In or172, or180 and or187; maternal-effect
FT embryonic lethal."
FT /evidence="ECO:0000269|PubMed:11861581"
FT MUTAGEN 502
FT /note="S->F: In or180; embryonic lethal."
FT /evidence="ECO:0000269|PubMed:11134076,
FT ECO:0000269|PubMed:17057243"
FT MUTAGEN 528
FT /note="A->T: In ax148; maternal-effect embryonic lethal."
FT /evidence="ECO:0000269|PubMed:11861581"
FT MUTAGEN 532
FT /note="G->D: In ax71; results in sterility."
FT /evidence="ECO:0000269|PubMed:11861581"
SQ SEQUENCE 673 AA; 76993 MW; D76FAA6D4892E5FA CRC64;
MNVSFSTPVQ NSTASRLALI QQAAARSGAL PPAGQYRGSK LSPFDVSQMS GVSISMVERA
KINGPEFVEE LEWLRQQTTS RCFLDAEMWT NEILAHLPDK WCAPNTLNLY NQVSELVLDN
TRSPMASPAS NSLYAPGEDQ MPTVKRNHTS RFAQSLIKNK EFRRAAFFLE KTMNGNKLDH
FLHFRCLFLA YYQEHLENDA EGIERKTSFA EERSPFSLLY QRMEDKKLRE NEDVWFEYLM
GLLEVELGLK DLAEKSFRNV VIREPRIWPA WEALSRLIAD IEDADKFVTS AEVKSLWMGD
WFMTLVLQRF HQHSMAIQKA EQLVTRGMTG LPMIITKIAA CSNARHDHDQ AISNFEDVRK
ADPYRLGDLH LLSDSLYIRN DQKKLSTLAI EVYKVHKFRW ETCCIVANYH AIRRDSEHAI
KFFQRALRLN PGLAALWVLI GHEFMEMKNN AAACVSYRRA IEIDPADHRG WYGLGQMYDI
MKMPAYALFY YQEAQKCKPH DSRLLVALGD IYSKLNRIED AEKCFTGAYL FGDVEGNALW
SLAKLHERYS DDNKAAQAFE VFLVVYELVT SAEEKIIYAI AFLANHFFKI EDFDKASEYA
TKCLAFETLC QEGNRLFREI AKIQARESRL PVEEAPGPSN ASAAGGQEAM DTEEAPQEGG
EEEMSEGEDD FSF
