ID   CDC25_LACKL             Reviewed;        1095 AA.
AC   Q02342;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Cell division control protein 25;
DE   Flags: Fragment;
GN   Name=CDC25;
OS   Lachancea kluyveri (Yeast) (Saccharomyces kluyveri).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Lachancea.
OX   NCBI_TaxID=4934;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1644315; DOI=10.1016/0378-1119(92)90491-7;
RA   Prigozy T., Gonzales E., Broek D.;
RT   "Identification and analysis of a DNA fragment from Saccharomyces kluyveri
RT   that can complement the loss of CDC25 function in Saccharomyces
RT   cerevisiae.";
RL   Gene 117:67-72(1992).
CC   -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. This protein
CC       positively controls the level of cellular cAMP at start, the stage at
CC       which the yeast cell division cycle is triggered.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; M82964; AAA34479.1; -; Genomic_DNA.
DR   PIR; PC1114; PC1114.
DR   AlphaFoldDB; Q02342; -.
DR   SMR; Q02342; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   PANTHER; PTHR23113; PTHR23113; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   PROSITE; PS00720; RASGEF; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
PE   4: Predicted;
KW   Cell cycle; Cell division; Guanine-nucleotide releasing factor; Membrane;
KW   Mitosis; Transmembrane; Transmembrane helix.
FT   CHAIN           <1..1095
FT                   /note="Cell division control protein 25"
FT                   /id="PRO_0000068862"
FT   TRANSMEM        959..980
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          626..756
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT   DOMAIN          812..1049
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT   REGION          103..133
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          158..178
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          263..295
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          309..333
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          773..798
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1044..1078
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        309..328
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1044..1073
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_TER         1
SQ   SEQUENCE   1095 AA;  125606 MW;  7A8B2F90E31A44AC CRC64;
     SWTEFRNSTL HYINLAQEHF FKNNQHEFNT SFTMATRLIA FHQLGCRLLK HEMVKKNLRR
     EARRLLKKLI TSMARIGVNS NLFFCSPQRI SLINQLAASR QSDPRDSKVS ITSTNTTIPD
     PSRSSASTIP SFLNQESPAR PIRNFSSSTT DTLMFSEDWQ SNSPNLESPQ EIPSTRTSVP
     SLNAPLSFSV IDDESGILVQ SLFQVIDTEF FRFKKNVVKL HDLMQAHFGQ GDLPQLFPRF
     FKGHFNEGAW TNPFHQGYEY GSAQNSDSSP VFDSSQQQAN DSLGNRSYSV PSKDHTSFRG
     FSVSSKATST ASSSNAKHSI TGKPSHTRTF SRAKIMKRNR YPLNGDTLNL LKKKFNFIII
     NYYGNDHRLI DQPKSKKRNL ELSADTYKAL NYIVDMIEVL ENLDLTFFVN LRNMSHDEKL
     DRESEELRKH AMTSLASTLM EFFEIKQALH DIAIRQIMDV QGLTLDDPFV FCAIRDESVC
     YFDREDELSK YQFLKQEKFA EELRNLLISQ DVEYNDLAFL DADQLVREAF AKFSDIGNTM
     LQNVEQLIEE RENILNYAAR MMKSDLTAAL MKGEQEKWFE DEDFDMASSA EGNENLDFGD
     AQNKSFSRDI PWYLDSEHEY SLIYDNKGNI KGGTKEALLE HLTSHQSIDL SFNLAMLLTF
     RSIFTTGEFL QALVERYNLY PPEGVSYEEY NIWVEKKQKP VKIRVVNIMK TLFSQYWTPS
     YYEPGLDYMI GFAQLAKSQK ISGADVLLSA IKGRLSMKGN LKNFVPESIN FSDDGSSTTT
     VPQSSRSSVS APVGSSSTTG FRMRKLKLLD IDSLDYAKQL TIKEHSLFYK ISPFECLDRT
     WGNKYCNMGG SKNITEFISN SNHLTNYVSF MIVKQTDIKK RIQLIQFFIN VAAHCHELNN
     FSSLTAIISA LYSSPIYRLK RTWAAVPEEY KKLLEELNTL MDSAKNFIRY RQLLKSIGDF
     PCVPFFGVYL SDLTFTANGN PDFLHRNTVL VNFGKRVRIL EILKEISVYQ RSHYKLKRYE
     DIQAFIESSL ENLPSIEKQY AQSLRNEPRT EVSTGLNSTN VNYRYNTKNG PENRNTGKRL
     KFGKAKKQSA NLLSQ