CDC26_YEAST
ID CDC26_YEAST Reviewed; 124 AA.
AC P14724; D6VTR9;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Anaphase-promoting complex subunit CDC26;
DE AltName: Full=Cell division control protein 26;
GN Name=CDC26; Synonyms=HIT3, SCD26; OrderedLocusNames=YFR036W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2690018; DOI=10.1093/nar/17.24.10491;
RA Dascher C., Kuentzel H.;
RT "Nucleotide sequence of yeast SCD26, a dosage-dependent suppressor of the
RT cell cycle mutation cdc26.";
RL Nucleic Acids Res. 17:10491-10491(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=8686381;
RX DOI=10.1002/(sici)1097-0061(199602)12:2<177::aid-yea896>3.0.co;2-a;
RA Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M.,
RA Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H.,
RA Hanaoka F., Murakami Y.;
RT "Fifteen open reading frames in a 30.8 kb region of the right arm of
RT chromosome VI from Saccharomyces cerevisiae.";
RL Yeast 12:177-190(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 47-54.
RC STRAIN=P-28-24C;
RX PubMed=1736102; DOI=10.1007/bf00279807;
RA Araki H., Awane K., Ogawa N., Oshima Y.;
RT "The CDC26 gene of Saccharomyces cerevisiae is required for cell growth
RT only at high temperature.";
RL Mol. Gen. Genet. 231:329-331(1992).
RN [7]
RP SUBUNIT.
RX PubMed=8895471; DOI=10.1126/science.274.5290.1201;
RA Zachariae W., Shin T.H., Galova M., Obermaier B., Nasmyth K.;
RT "Identification of subunits of the anaphase-promoting complex of
RT Saccharomyces cerevisiae.";
RL Science 274:1201-1204(1996).
RN [8]
RP SUBUNIT.
RX PubMed=15060174; DOI=10.1128/mcb.24.8.3562-3576.2004;
RA Schwickart M., Havlis J., Habermann B., Bogdanova A., Camasses A.,
RA Oelschlaegel T., Shevchenko A., Zachariae W.;
RT "Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-
RT promoting complex stabilizing the association of Cdc16 and Cdc27.";
RL Mol. Cell. Biol. 24:3562-3576(2004).
RN [9]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Component of the anaphase promoting complex/cyclosome
CC (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex
CC that controls progression through mitosis and the G1 phase of the cell
CC cycle. The APC/C is thought to confer substrate specificity and, in the
CC presence of ubiquitin-conjugating E2 enzymes, it catalyzes the
CC formation of protein-ubiquitin conjugates that are subsequently
CC degraded by the 26S proteasome. In early mitosis, the APC/C is
CC activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5,
CC and other anaphase inhibitory proteins for proteolysis, thereby
CC triggering the separation of sister chromatids at the metaphase-to-
CC anaphase transition. In late mitosis and in G1, degradation of CLB5
CC allows activation of the APC/C by CDH1, which is needed to destroy
CC CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and
CC creating the low CDK state necessary for cytokinesis and for reforming
CC prereplicative complexes in G1 prior to another round of replication.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: The APC/C is composed of at least 13 subunits that stay
CC tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5,
CC APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1. CDC26 is
CC required to tether the essential subunits APC9, CDC27 and CDC16 to the
CC complex. {ECO:0000269|PubMed:15060174, ECO:0000269|PubMed:8895471}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- SIMILARITY: Belongs to the CDC26 family. {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be a suppressor of CDC26.
CC {ECO:0000305|PubMed:2690018}.
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DR EMBL; X17118; CAA34978.1; -; Genomic_DNA.
DR EMBL; D50617; BAA09275.1; -; Genomic_DNA.
DR EMBL; AY558473; AAS56799.1; -; Genomic_DNA.
DR EMBL; S79900; AAB21293.1; -; Genomic_DNA.
DR EMBL; BK006940; DAA12479.1; -; Genomic_DNA.
DR PIR; S56291; COBY26.
DR RefSeq; NP_116694.1; NM_001180001.1.
DR AlphaFoldDB; P14724; -.
DR SMR; P14724; -.
DR BioGRID; 31194; 126.
DR ComplexPortal; CPX-756; Anaphase-Promoting core complex.
DR ComplexPortal; CPX-760; Anaphase-Promoting Complex, CDC20 variant.
DR ComplexPortal; CPX-761; Anaphase-Promoting Complex, CDH1 variant.
DR ComplexPortal; CPX-762; Anaphase-Promoting complex AMA1 variant.
DR DIP; DIP-352N; -.
DR IntAct; P14724; 13.
DR MINT; P14724; -.
DR STRING; 4932.YFR036W; -.
DR iPTMnet; P14724; -.
DR PaxDb; P14724; -.
DR PRIDE; P14724; -.
DR EnsemblFungi; YFR036W_mRNA; YFR036W; YFR036W.
DR GeneID; 850597; -.
DR KEGG; sce:YFR036W; -.
DR SGD; S000001932; CDC26.
DR VEuPathDB; FungiDB:YFR036W; -.
DR HOGENOM; CLU_140545_0_0_1; -.
DR InParanoid; P14724; -.
DR BioCyc; YEAST:G3O-30483-MON; -.
DR UniPathway; UPA00143; -.
DR ChiTaRS; CDC26; yeast.
DR PRO; PR:P14724; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P14724; protein.
DR GO; GO:0005680; C:anaphase-promoting complex; IDA:SGD.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IDA:SGD.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0031497; P:chromatin assembly; IGI:SGD.
DR GO; GO:0016567; P:protein ubiquitination; IDA:ComplexPortal.
DR GO; GO:0051445; P:regulation of meiotic cell cycle; IC:ComplexPortal.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; IC:ComplexPortal.
DR GO; GO:0030071; P:regulation of mitotic metaphase/anaphase transition; IEA:InterPro.
DR InterPro; IPR018860; APC_suCDC26.
DR Pfam; PF10471; ANAPC_CDC26; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Mitosis; Nucleus; Phosphoprotein;
KW Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..124
FT /note="Anaphase-promoting complex subunit CDC26"
FT /id="PRO_0000089446"
FT REGION 40..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 99..124
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..65
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 99..117
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 12
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT CONFLICT 50
FT /note="S -> F (in Ref. 1; CAA34978)"
FT /evidence="ECO:0000305"
FT CONFLICT 121..124
FT /note="IREE -> TARNNSISAYAHAHAHAHASTFTFTYKISSL (in Ref. 1;
FT CAA34978)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 124 AA; 14081 MW; F41EECD2B56C4FB5 CRC64;
MIRRAPTTLQ LSHDDVTSLI DDLNEQKLKQ QLNIEKTKYF QGKNGGSLHS NTDFQDTSQN
IEDNNNDNDN DIDEDDDMSS YNDKAASVAH TRVLNSLHLS TDSNTAHETS NANDNHNPFY
IREE
