CDC45_MOUSE
ID CDC45_MOUSE Reviewed; 566 AA.
AC Q9Z1X9; O70547; Q9QUK1; Q9R212;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Cell division control protein 45 homolog;
DE AltName: Full=PORC-PI-1;
GN Name=Cdc45; Synonyms=Cdc45l, Cdc45l2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Breyer P., Staib C., Lepke M., Springer J., Grummt F.;
RL Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9724329; DOI=10.1101/gr.8.8.834;
RA McKie J.M., Wadey R.B., Sutherland H.F., Taylor C.L., Scambler P.J.;
RT "Direct selection of conserved cDNAs from the DiGeorge critical region:
RT isolation of a novel CDC45-like gene.";
RL Genome Res. 8:834-841(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Ectoplacental cone, and Testis;
RX PubMed=10051334; DOI=10.1007/s003359900996;
RA Shaikh T.H., Gottlieb S., Sellinger B., Chen F., Roe B.A., Oakey R.J.,
RA Emanuel B.S., Budarf M.L.;
RT "Characterization of CDC45L: a gene in the 22q11.2 deletion region
RT expressed during murine and human development.";
RL Mamm. Genome 10:322-326(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144 AND SER-146, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Required for initiation of chromosomal DNA replication.
CC -!- SUBUNIT: Associated with ORC2. Interacts with HELB (By similarity).
CC Component of the CMG helicase complex, composed of the MCM2-7 complex,
CC the GINS complex and CDC45 (By similarity).
CC {ECO:0000250|UniProtKB:O75419, ECO:0000250|UniProtKB:Q9YHZ6}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Widely expressed.
CC -!- SIMILARITY: Belongs to the CDC45 family. {ECO:0000305}.
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DR EMBL; AF098068; AAC95057.1; -; mRNA.
DR EMBL; AJ223729; CAA11531.1; -; mRNA.
DR EMBL; AF081536; AAD08999.1; -; mRNA.
DR EMBL; AF081537; AAD09000.1; -; mRNA.
DR EMBL; AF081538; AAD09001.1; -; mRNA.
DR EMBL; AF081539; AAD09002.1; -; mRNA.
DR EMBL; BC028635; AAH28635.1; -; mRNA.
DR CCDS; CCDS28027.1; -.
DR RefSeq; NP_033992.2; NM_009862.2.
DR RefSeq; XP_006521807.1; XM_006521744.2.
DR RefSeq; XP_011244115.1; XM_011245813.2.
DR AlphaFoldDB; Q9Z1X9; -.
DR SMR; Q9Z1X9; -.
DR BioGRID; 198628; 2.
DR ComplexPortal; CPX-4541; CMG helicase complex.
DR IntAct; Q9Z1X9; 2.
DR STRING; 10090.ENSMUSP00000000028; -.
DR iPTMnet; Q9Z1X9; -.
DR PhosphoSitePlus; Q9Z1X9; -.
DR EPD; Q9Z1X9; -.
DR MaxQB; Q9Z1X9; -.
DR PaxDb; Q9Z1X9; -.
DR PRIDE; Q9Z1X9; -.
DR ProteomicsDB; 283765; -.
DR Antibodypedia; 262; 370 antibodies from 37 providers.
DR DNASU; 12544; -.
DR Ensembl; ENSMUST00000000028; ENSMUSP00000000028; ENSMUSG00000000028.
DR GeneID; 12544; -.
DR KEGG; mmu:12544; -.
DR UCSC; uc007yom.2; mouse.
DR CTD; 8318; -.
DR MGI; MGI:1338073; Cdc45.
DR VEuPathDB; HostDB:ENSMUSG00000000028; -.
DR eggNOG; KOG2475; Eukaryota.
DR GeneTree; ENSGT00390000009662; -.
DR HOGENOM; CLU_005871_4_0_1; -.
DR InParanoid; Q9Z1X9; -.
DR OMA; PWNLANV; -.
DR OrthoDB; 1309031at2759; -.
DR PhylomeDB; Q9Z1X9; -.
DR TreeFam; TF101062; -.
DR Reactome; R-MMU-176187; Activation of ATR in response to replication stress.
DR Reactome; R-MMU-68962; Activation of the pre-replicative complex.
DR BioGRID-ORCS; 12544; 29 hits in 111 CRISPR screens.
DR ChiTaRS; Cdc45; mouse.
DR PRO; PR:Q9Z1X9; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9Z1X9; protein.
DR Bgee; ENSMUSG00000000028; Expressed in animal zygote and 172 other tissues.
DR ExpressionAtlas; Q9Z1X9; baseline and differential.
DR Genevisible; Q9Z1X9; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0036064; C:ciliary basal body; ISO:MGI.
DR GO; GO:0071162; C:CMG complex; ISO:MGI.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; ISO:MGI.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR GO; GO:1902977; P:mitotic DNA replication preinitiation complex assembly; IBA:GO_Central.
DR InterPro; IPR003874; CDC45.
DR PANTHER; PTHR10507; PTHR10507; 1.
DR Pfam; PF02724; CDC45; 2.
PE 1: Evidence at protein level;
KW Cell cycle; DNA replication; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..566
FT /note="Cell division control protein 45 homolog"
FT /id="PRO_0000192816"
FT REGION 138..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 130
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O75419"
FT MOD_RES 144
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 146
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 142
FT /note="D -> Y (in Ref. 1; AAC95057)"
FT /evidence="ECO:0000305"
FT CONFLICT 168..177
FT /note="NRKRRQRREW -> ETGRGGRPGSG (in Ref. 2; CAA11531)"
FT /evidence="ECO:0000305"
FT CONFLICT 175
FT /note="R -> K (in Ref. 3; AAD08999/AAD09000/AAD09001)"
FT /evidence="ECO:0000305"
FT CONFLICT 197
FT /note="S -> T (in Ref. 2; CAA11531)"
FT /evidence="ECO:0000305"
FT CONFLICT 249
FT /note="V -> L (in Ref. 2; CAA11531)"
FT /evidence="ECO:0000305"
FT CONFLICT 337..342
FT /note="KGNLRE -> EGESAR (in Ref. 2; CAA11531)"
FT /evidence="ECO:0000305"
FT CONFLICT 373
FT /note="A -> T (in Ref. 2; CAA11531)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 566 AA; 65384 MW; 9BA69F0EA9CFAD9A CRC64;
MFVTDFRKEF YETVHNQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET
AYLEHKEQFS YFILINCGAN VDLLDILQPD EDSIFFVCDT HRPVNVVNVY NDTQIKLLIK
QEDDLEVPAY DDIFRDEAED EDLSDSDGDG SEPSEKRTRL EEEIVERNRK RRQRREWEAR
RKDILFDYEQ YEYYGTSSAM VMFDLAWMMS KDLNDMLWWA IVGLTDQWVH DKITQMKYVT
DVGILQRHVS RHNHRNEAEE NMLSVDCTRI SFEYDLCLVL YQHWSLHESL YNTSYTAARF
KLWSVHGQKR LQEFLADMGL PLKQVKQKFQ SMDVSLKGNL REMIEESANK FGMKDMRVQT
FSIQFGFKHK FLASDVVFAT MSLMESPEKD GSGTDHFIQA LDSLSRSNLD KLYLGLELAK
KHLQATQQTI ASCLCTNLVT SQGPFLYCSL MEGTPDVTLF SKPASLSLLS RHLLKSFVYS
TKNRRCKLLP LVMAAPLSVE QGTVTVVGIP PETDSSDRKN FFGRAFEKAA ESTSSRTLHN
YFDLSVIELK AEDRSKFLDA LVSLLS
