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CDC61_METTH
ID   CDC61_METTH             Reviewed;         382 AA.
AC   O27463;
DT   19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=ORC1-type DNA replication protein 1 {ECO:0000255|HAMAP-Rule:MF_01407};
GN   Name=cdc6-1; OrderedLocusNames=MTH_1412;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
RN   [2]
RP   PHOSPHORYLATION, AND MUTAGENESIS OF ASP-149.
RX   PubMed=11514535; DOI=10.1128/jb.183.18.5459-5464.2001;
RA   Grabowski B., Kelman Z.;
RT   "Autophosphorylation of archaeal Cdc6 homologues is regulated by DNA.";
RL   J. Bacteriol. 183:5459-5464(2001).
RN   [3]
RP   FUNCTION, DNA-BINDING, SUBUNIT, AND MUTAGENESIS OF ARG-334.
RX   PubMed=15358831; DOI=10.1093/nar/gkh819;
RA   Capaldi S.A., Berger J.M.;
RT   "Biochemical characterization of Cdc6/Orc1 binding to the replication
RT   origin of the euryarchaeon Methanothermobacter thermoautotrophicus.";
RL   Nucleic Acids Res. 32:4821-4832(2004).
RN   [4]
RP   FUNCTION, DNA-BINDING, INTERACTION WITH MCM, AND PHOSPHORYLATION.
RX   PubMed=16150924; DOI=10.1093/nar/gki807;
RA   Kasiviswanathan R., Shin J.H., Kelman Z.;
RT   "Interactions between the archaeal Cdc6 and MCM proteins modulate their
RT   biochemical properties.";
RL   Nucleic Acids Res. 33:4940-4950(2005).
RN   [5]
RP   FUNCTION, DNA-BINDING, INTERACTION WITH MCM, AND DOMAIN.
RX   PubMed=16740965; DOI=10.1128/jb.00168-06;
RA   Kasiviswanathan R., Shin J.H., Kelman Z.;
RT   "DNA binding by the Methanothermobacter thermautotrophicus Cdc6 protein is
RT   inhibited by the minichromosome maintenance helicase.";
RL   J. Bacteriol. 188:4577-4580(2006).
RN   [6]
RP   FUNCTION.
RX   PubMed=18390662; DOI=10.1128/jb.00233-08;
RA   Shin J.H., Heo G.Y., Kelman Z.;
RT   "The Methanothermobacter thermautotrophicus Cdc6-2 protein, the putative
RT   helicase loader, dissociates the minichromosome maintenance helicase.";
RL   J. Bacteriol. 190:4091-4094(2008).
CC   -!- FUNCTION: Involved in regulation of DNA replication. May play an
CC       essential role in origin recognition. Binds to DNA, with a preference
CC       for origin-specific double-stranded sequences. Does not bind single-
CC       stranded DNA. Inhibits MCM helicase activity but does not affect its
CC       oligomeric state. {ECO:0000255|HAMAP-Rule:MF_01407,
CC       ECO:0000269|PubMed:15358831, ECO:0000269|PubMed:16150924,
CC       ECO:0000269|PubMed:16740965, ECO:0000269|PubMed:18390662}.
CC   -!- SUBUNIT: Monomer. Interacts with MCM via the WH domain.
CC       {ECO:0000269|PubMed:15358831, ECO:0000269|PubMed:16150924,
CC       ECO:0000269|PubMed:16740965}.
CC   -!- DOMAIN: The N-terminal AAA+ ATPase domain and the C-terminal winged-
CC       helix (WH) domain are both required for DNA binding.
CC       {ECO:0000269|PubMed:16740965}.
CC   -!- PTM: Autophosphorylated on a serine. Phosphorylation is stimulated by
CC       binding to MCM. Both single-stranded DNA and double-stranded DNA
CC       inhibit the phosphorylation reaction. {ECO:0000269|PubMed:11514535,
CC       ECO:0000269|PubMed:16150924}.
CC   -!- SIMILARITY: Belongs to the CDC6/cdc18 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01407}.
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DR   EMBL; AE000666; AAB85889.1; -; Genomic_DNA.
DR   PIR; B69055; B69055.
DR   RefSeq; WP_010877024.1; NC_000916.1.
DR   AlphaFoldDB; O27463; -.
DR   SMR; O27463; -.
DR   STRING; 187420.MTH_1412; -.
DR   EnsemblBacteria; AAB85889; AAB85889; MTH_1412.
DR   GeneID; 24854525; -.
DR   KEGG; mth:MTH_1412; -.
DR   PATRIC; fig|187420.15.peg.1376; -.
DR   HOGENOM; CLU_025112_3_1_2; -.
DR   OMA; DMLGIIN; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   CDD; cd08768; Cdc6_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01407; ORC1_type_DNA_replic_protein; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR015163; Cdc6_C.
DR   InterPro; IPR014277; Orc1/Cdc6_arc.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF13401; AAA_22; 1.
DR   Pfam; PF09079; Cdc6_C; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM01074; Cdc6_C; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02928; TIGR02928; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA replication; DNA-binding; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..382
FT                   /note="ORC1-type DNA replication protein 1"
FT                   /id="PRO_0000151008"
FT   BINDING         63..67
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01407"
FT   BINDING         205
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01407"
FT   BINDING         217
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01407"
FT   MUTAGEN         149
FT                   /note="D->N: No labeling with ATP."
FT                   /evidence="ECO:0000269|PubMed:11514535"
FT   MUTAGEN         334
FT                   /note="R->A: Abolishes DNA binding."
FT                   /evidence="ECO:0000269|PubMed:15358831"
SQ   SEQUENCE   382 AA;  43875 MW;  FE81374BE134961C CRC64;
     MNIFDEIGDK ESVFKDKKYL DHRFLPDRLP HREEQIRSIA KYWVEALNGV TPPDITIYGK
     TGTGKTAVAK FAMKQLKEAS KDCDVNIRTE YIRCTDYTTE YQVIARLCQQ LGRDVPYRGW
     TKAEIVNTFR NMFKKNAFGQ DMILMVVLDE IDILLRNDGD GLLYTLTRTD NVSILSISNY
     VEFKKFIKPR VRSSLRDREI VFPPYGAQQL VDILEERSKM SFKEGALDDD VIPLCAALAA
     KEEGDARYAL DLLRTAGEIA DERDSDKVLG DFVREAKDYI EHNKITDIIL TLPSQQQRVL
     EAILYLTKRK EEITSGRLYE VYKEIAKGDS VSYRRIFDFI NELEMLGLIS TNTVSRGRGK
     GRTNIIDLQC ETSLLEDSLW GV
 
 
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