CDC6_PYRFU
ID CDC6_PYRFU Reviewed; 420 AA.
AC P81413;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=ORC1-type DNA replication protein {ECO:0000255|HAMAP-Rule:MF_01407};
GN Name=cdc6; Synonyms=orc1; OrderedLocusNames=PF0017;
OS Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=186497;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=9348040; DOI=10.1046/j.1365-2443.1997.1380336.x;
RA Uemori T., Sato Y., Kato I., Doi H., Ishino Y.;
RT "A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus
RT furiosus: gene cloning, expression, and characterization.";
RL Genes Cells 2:499-512(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA DiRuggiero J., Robb F.T.;
RT "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT horikoshii inferred from complete genomic sequences.";
RL Genetics 152:1299-1305(1999).
RN [3]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=17452353; DOI=10.1093/nar/gkm212;
RA Matsunaga F., Glatigny A., Mucchielli-Giorgi M.H., Agier N., Delacroix H.,
RA Marisa L., Durosay P., Ishino Y., Aggerbeck L., Forterre P.;
RT "Genomewide and biochemical analyses of DNA-binding activity of Cdc6/Orc1
RT and Mcm proteins in Pyrococcus sp.";
RL Nucleic Acids Res. 35:3214-3222(2007).
RN [4]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=19787415; DOI=10.1007/s00792-009-0284-9;
RA Matsunaga F., Takemura K., Akita M., Adachi A., Yamagami T., Ishino Y.;
RT "Localized melting of duplex DNA by Cdc6/Orc1 at the DNA replication origin
RT in the hyperthermophilic archaeon Pyrococcus furiosus.";
RL Extremophiles 14:21-31(2010).
RN [5]
RP FUNCTION, DNA-BINDING, SUBUNIT, AND INTERACTION WITH MCM.
RX PubMed=20384788; DOI=10.1111/j.1365-2443.2010.01402.x;
RA Akita M., Adachi A., Takemura K., Yamagami T., Matsunaga F., Ishino Y.;
RT "Cdc6/Orc1 from Pyrococcus furiosus may act as the origin recognition
RT protein and Mcm helicase recruiter.";
RL Genes Cells 15:537-552(2010).
CC -!- FUNCTION: Involved in regulation of DNA replication. May play essential
CC roles in origin recognition and cell cycle control of replication.
CC Binds specifically to the oriC region, which alters the topological
CC structure of the DNA and introduces localized melting of the DNA
CC duplex. May recruit the MCM helicase onto the oriC region. Shows weak
CC ATPase activity in the absence of DNA. {ECO:0000255|HAMAP-
CC Rule:MF_01407, ECO:0000269|PubMed:17452353,
CC ECO:0000269|PubMed:19787415, ECO:0000269|PubMed:20384788}.
CC -!- SUBUNIT: Homodimer (Probable). Interacts with MCM.
CC {ECO:0000269|PubMed:20384788, ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CDC6/cdc18 family. {ECO:0000255|HAMAP-
CC Rule:MF_01407}.
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DR EMBL; D84670; BAA25162.1; -; Genomic_DNA.
DR EMBL; AE009950; AAL80141.1; -; Genomic_DNA.
DR PIR; T43932; T43932.
DR RefSeq; WP_011011129.1; NC_018092.1.
DR AlphaFoldDB; P81413; -.
DR SMR; P81413; -.
DR STRING; 186497.PF0017; -.
DR EnsemblBacteria; AAL80141; AAL80141; PF0017.
DR GeneID; 41711803; -.
DR KEGG; pfu:PF0017; -.
DR PATRIC; fig|186497.12.peg.19; -.
DR eggNOG; arCOG00467; Archaea.
DR HOGENOM; CLU_025112_3_1_2; -.
DR OMA; DMLGIIN; -.
DR OrthoDB; 58376at2157; -.
DR PhylomeDB; P81413; -.
DR Proteomes; UP000001013; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08768; Cdc6_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01407; ORC1_type_DNA_replic_protein; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR015163; Cdc6_C.
DR InterPro; IPR014277; Orc1/Cdc6_arc.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF09079; Cdc6_C; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM01074; Cdc6_C; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02928; TIGR02928; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA replication; DNA-binding; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..420
FT /note="ORC1-type DNA replication protein"
FT /id="PRO_0000151012"
FT BINDING 73..77
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01407"
FT BINDING 221
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01407"
FT BINDING 233
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01407"
SQ SEQUENCE 420 AA; 48247 MW; BAE9CE03E61F39C5 CRC64;
MNEGEHQIKL DELFEKLLRA RKIFKNKDVL RHSYTPKDLP HRHEQIETLA QILVPVLRGE
TPSNIFVYGK TGTGKTVTVK FVTEELKRIS EKYNIPVDVI YINCEIVDTH YRVLANIVNY
FKDETGIEVP MVGWPTDEVY AKLKQVIDMK ERFVIIVLDE IDKLVKKSGD EVLYSLTRIN
TELKRAKVSV IGISNDLKFK EYLDPRVLSS LSEEEVVFPP YDANQLRDIL TQRAEEAFYP
GVLDEGVIPL CAALAAREHG DARKALDLLR VAGEIAEREG ASKVTEKHVW KAQEKIEQDM
MEEVIKTLPL QSKVLLYAIV LLDENGDLPA NTGDVYAVYR ELCEYIDLEP LTQRRISDLI
NELDMLGIIN AKVVSKGRYG RTKEIRLNVT SYKIRNVLRY DYSIQPLLTI SLKSEQRRLI
