ID   CDCA3_XENLA             Reviewed;         363 AA.
AC   P0C2X8;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Cell division cycle-associated protein 3;
DE   AltName: Full=Trigger of mitotic entry protein 1;
DE            Short=TOME-1;
GN   Name=cdca3; Synonyms=tome1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION,
RP   PHOSPHORYLATION, UBIQUITINATION, DEVELOPMENTAL STAGE, AND INTERACTION WITH
RP   WEE1.
RX   PubMed=12679038; DOI=10.1016/s0092-8674(03)00232-0;
RA   Ayad N.G., Rankin S., Murakami M., Jebanathirajah J., Gygi S.P.,
RA   Kirschner M.W.;
RT   "Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC.";
RL   Cell 113:101-113(2003).
CC   -!- FUNCTION: F-box-like protein which is required for entry into mitosis.
CC       Acts by participating in E3 ligase complexes that mediate the
CC       ubiquitination and degradation of WEE1 kinase at G2/M phase.
CC       {ECO:0000269|PubMed:12679038}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Interacts with wee1, when wee1 is phosphorylated at 'Ser-38'.
CC       {ECO:0000269|PubMed:12679038}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:12679038}.
CC   -!- DEVELOPMENTAL STAGE: Present at high level in G2 and M phases but
CC       declines rapidly in G2 phase (at protein level).
CC       {ECO:0000269|PubMed:12679038}.
CC   -!- DOMAIN: The KEN box is required for the association with the APC/C-Cdh1
CC       complex.
CC   -!- PTM: Phosphorylated. {ECO:0000269|PubMed:12679038}.
CC   -!- PTM: Ubiquitinated and degraded by the APC/C-Cdh1 complex during G1
CC       phase. {ECO:0000269|PubMed:12679038}.
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DR   AlphaFoldDB; P0C2X8; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000186698; Genome assembly.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR038832; CDCA3.
DR   PANTHER; PTHR34756; PTHR34756; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Cytoplasm; Mitosis; Phosphoprotein;
KW   Reference proteome; Ubl conjugation; Ubl conjugation pathway.
FT   CHAIN           1..363
FT                   /note="Cell division cycle-associated protein 3"
FT                   /id="PRO_0000287711"
FT   REGION          1..81
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          93..152
FT                   /note="F-box-like"
FT   REGION          126..152
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          191..210
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          231..363
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           353..355
FT                   /note="KEN box"
FT   COMPBIAS        1..32
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        137..151
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        252..286
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        293..327
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        345..363
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   363 AA;  39702 MW;  9BE5780A53A622FD CRC64;
     MGSAESKAQV TPSRPLRNHL LSRVNDPRSP TSGIPRTPIE VGESPRNTPQ TVKEEEEEIP
     DSPEIFDPRS PTNGITRTPL RPPIHAVLNN LAKQLSEVFV AEDSSTEGGP LGFTGPEATN
     LERQVVESQT APPAGEHVND HEVEPSVEKA ETQIDLEVCP GVEKVKSPIA EMLETLNDQE
     ESPIAETLET MNDQEESPIA ETMNDQEESP IAETLENLND QAESPIAETL ENLNDQAESP
     IAEMLDTLND QEPVAVAQSV VSTESTQATG QQQKTRGKSP RSSGVKNVRQ RPRKALLSSS
     SGRSPLRILQ EDNSPNTNTQ HRQAKKLSFQ SEPALPHRAL KISHPNWESS LNKENAEYGH
     SNS