CDCA7_MOUSE
ID CDCA7_MOUSE Reviewed; 382 AA.
AC Q9D0M2; Q3TIS1; Q3U511; Q6NZE5; Q8C1A0;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Cell division cycle-associated protein 7;
GN Name=Cdca7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, Placenta, Skin, Thymus, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Egg;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-203, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic brain;
RX PubMed=15345747; DOI=10.1074/mcp.m400085-mcp200;
RA Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
RT "Phosphoproteomic analysis of the developing mouse brain.";
RL Mol. Cell. Proteomics 3:1093-1101(2004).
RN [4]
RP TRANSGENIC MICE.
RX PubMed=15994934; DOI=10.1158/0008-5472.can-05-0536;
RA Osthus R.C., Karim B., Prescott J.E., Smith B.D., McDevitt M., Huso D.L.,
RA Dang C.V.;
RT "The Myc target gene JPO1/CDCA7 is frequently overexpressed in human tumors
RT and has limited transforming activity in vivo.";
RL Cancer Res. 65:5620-5627(2005).
RN [5]
RP FUNCTION.
RX PubMed=16580749; DOI=10.1016/j.bbaexp.2006.02.004;
RA Goto Y., Hayashi R., Muramatsu T., Ogawa H., Eguchi I., Oshida Y.,
RA Ohtani K., Yoshida K.;
RT "JPO1/CDCA7, a novel transcription factor E2F1-induced protein, possesses
RT intrinsic transcriptional regulator activity.";
RL Biochim. Biophys. Acta 1759:60-68(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Participates in MYC-mediated cell transformation and
CC apoptosis; induces anchorage-independent growth and clonogenicity in
CC lymphoblastoid cells. Insufficient to induce tumorigenicity when
CC overexpressed but contributes to MYC-mediated tumorigenesis. May play a
CC role as transcriptional regulator (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:16580749}.
CC -!- SUBUNIT: Interacts with MYC (via C-terminus), YWHAE and YWHAZ.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC Note=Predominantly nuclear with some expression also seen in the
CC cytoplasm. Predominantly cytoplasmic when phosphorylated at Thr-170 (By
CC similarity). {ECO:0000250}.
CC -!- PTM: Phosphorylation at Thr-170 promotes interaction with YWHAE and
CC YWHAZ, dissociation from MYC and sequestration in the cytoplasm.
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: Transgenic mice overexpress Cdca7 in both T- and B-cell
CC compartments. At 1 year of age, they exhibit elevated incidence of
CC lymphomas, significant increased frequency of solid tumors and a 2-fold
CC increased risk of lymphoid malignancies compared to their littermates.
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DR EMBL; AK011289; BAB27519.1; -; mRNA.
DR EMBL; AK028671; BAC26058.1; -; mRNA.
DR EMBL; AK075882; BAC36027.1; -; mRNA.
DR EMBL; AK153943; BAE32269.1; -; mRNA.
DR EMBL; AK167734; BAE39775.1; -; mRNA.
DR EMBL; BC066169; AAH66169.1; -; mRNA.
DR CCDS; CCDS16121.1; -.
DR RefSeq; NP_080142.1; NM_025866.3.
DR AlphaFoldDB; Q9D0M2; -.
DR BioGRID; 211833; 2.
DR STRING; 10090.ENSMUSP00000099752; -.
DR iPTMnet; Q9D0M2; -.
DR PhosphoSitePlus; Q9D0M2; -.
DR EPD; Q9D0M2; -.
DR jPOST; Q9D0M2; -.
DR MaxQB; Q9D0M2; -.
DR PaxDb; Q9D0M2; -.
DR PeptideAtlas; Q9D0M2; -.
DR PRIDE; Q9D0M2; -.
DR ProteomicsDB; 281279; -.
DR Antibodypedia; 1585; 110 antibodies from 23 providers.
DR DNASU; 66953; -.
DR Ensembl; ENSMUST00000102691; ENSMUSP00000099752; ENSMUSG00000055612.
DR GeneID; 66953; -.
DR KEGG; mmu:66953; -.
DR UCSC; uc008kbx.1; mouse.
DR CTD; 83879; -.
DR MGI; MGI:1914203; Cdca7.
DR VEuPathDB; HostDB:ENSMUSG00000055612; -.
DR eggNOG; ENOG502QQPE; Eukaryota.
DR GeneTree; ENSGT00940000155436; -.
DR HOGENOM; CLU_035988_2_0_1; -.
DR InParanoid; Q9D0M2; -.
DR OMA; NICGSAR; -.
DR OrthoDB; 1462540at2759; -.
DR PhylomeDB; Q9D0M2; -.
DR TreeFam; TF101076; -.
DR BioGRID-ORCS; 66953; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Cdca7; mouse.
DR PRO; PR:Q9D0M2; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9D0M2; protein.
DR Bgee; ENSMUSG00000055612; Expressed in ventricular zone and 208 other tissues.
DR ExpressionAtlas; Q9D0M2; baseline and differential.
DR Genevisible; Q9D0M2; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0042127; P:regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR033576; CDCA7.
DR InterPro; IPR040221; CDCA7/CDA7L.
DR InterPro; IPR018866; Znf-4CXXC_R1.
DR PANTHER; PTHR31169; PTHR31169; 1.
DR PANTHER; PTHR31169:SF2; PTHR31169:SF2; 1.
DR Pfam; PF10497; zf-4CXXC_R1; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Cytoplasm; Isopeptide bond; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation;
KW Ubl conjugation.
FT CHAIN 1..382
FT /note="Cell division cycle-associated protein 7"
FT /id="PRO_0000249311"
FT REGION 68..118
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 151..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 152..177
FT /note="Interaction with MYC"
FT /evidence="ECO:0000250"
FT REGION 258..382
FT /note="Mediates transcriptional activity"
FT /evidence="ECO:0000250"
FT MOTIF 167..183
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 102..117
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 174..188
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 170
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9BWT1"
FT MOD_RES 197
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BWT1"
FT MOD_RES 203
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:15345747"
FT MOD_RES 225
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CROSSLNK 216
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9BWT1"
FT CONFLICT 100
FT /note="D -> N (in Ref. 1; BAC26058)"
FT /evidence="ECO:0000305"
FT CONFLICT 105
FT /note="S -> SE (in Ref. 1; BAE32269)"
FT /evidence="ECO:0000305"
FT CONFLICT 230
FT /note="M -> I (in Ref. 1; BAE39775)"
FT /evidence="ECO:0000305"
FT CONFLICT 360
FT /note="K -> R (in Ref. 2; AAH66169)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 382 AA; 43837 MW; 51920663588CDD40 CRC64;
MEARRARQKA LKVKNLKDVR YMKLISMETS SSSDDSCDSF ASDNFANTRL QLNREGCRTR
SQCRHSGPLR VAMKFPARNT RRAASKKAAP PKPSESSAND SHSDSEEEEE EEEEEDGMNF
LEKRALNIKQ NKAMLAKLMS ELESFPGLFS GRHSLPGHRA KDSKSPRRRT FPGVATRRNP
ERRTRPLTRS RSRILGSLGA LPTEEEEEEE EEEEDKYMLV RQRKSMDSYM NDDDVPRSRR
PGSMTLPHII RPVEEVTEEE IRNICSNSRE KIYNRSLGST CHQCRQKTTD TKTNCRNPDC
WGIRGQFCGP CLRNRYGEEV KDALLDPNWH CPPCRGICNC SFCRQRDGRC ATGVLVYLAK
YHGFGNVHAY LKSLKQEFEM QA
