ID   1A_TOBSV                Reviewed;        1094 AA.
AC   P89680;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Replication protein 1a;
DE   Includes:
DE     RecName: Full=ATP-dependent helicase;
DE              EC=3.6.4.-;
DE   Includes:
DE     RecName: Full=Methyltransferase;
DE              EC=2.1.1.-;
OS   Tobacco streak virus (strain WC) (TSV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Ilarvirus.
OX   NCBI_TaxID=12318;
OH   NCBI_TaxID=4686; Asparagus officinalis (Garden asparagus).
OH   NCBI_TaxID=41562; Dahlia.
OH   NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH   NCBI_TaxID=34274; Gossypium herbaceum (Levant cotton) (Arabian cotton).
OH   NCBI_TaxID=47082; Melilotus albus (White sweet clover) (Melilotus officinalis subsp. albus).
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH   NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH   NCBI_TaxID=137000; Rosa setigera.
OH   NCBI_TaxID=57577; Trifolium pratense (Red clover).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9687875; DOI=10.1007/s007050050366;
RA   Scott S.W., Zimmerman M.T., Ge X.;
RT   "The sequence of RNA 1 and RNA 2 of tobacco streak virus: additional
RT   evidence for the inclusion of alfalfa mosaic virus in the genus
RT   Ilarvirus.";
RL   Arch. Virol. 143:1187-1198(1998).
CC   -!- FUNCTION: Involved in the virus replication. Contains a helicase domain
CC       and a methyltransferase domain. The methyltransferase domain is
CC       probably involved in viral RNA capping. Involved in the formation of ER
CC       membrane spherular invaginations in which RNA replication complexes
CC       form (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with RNA-directed RNA polymerase 2a. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host endoplasmic reticulum membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the bromoviridae replication protein 1a family.
CC       {ECO:0000305}.
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DR   EMBL; U80934; AAB48983.1; -; mRNA.
DR   RefSeq; NP_620772.1; NC_003844.1.
DR   SMR; P89680; -.
DR   GeneID; 962658; -.
DR   KEGG; vg:962658; -.
DR   Proteomes; UP000007795; Genome.
DR   GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR002588; Alphavirus-like_MT_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   Pfam; PF01660; Vmethyltransf; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Helicase; Host endoplasmic reticulum; Host membrane;
KW   Hydrolase; Membrane; Methyltransferase; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..1094
FT                   /note="Replication protein 1a"
FT                   /id="PRO_0000402392"
FT   DOMAIN          88..279
FT                   /note="Alphavirus-like MT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT   DOMAIN          777..937
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          938..1093
FT                   /note="(+)RNA virus helicase C-terminal"
FT   REGION          68..409
FT                   /note="Methyltransferase"
FT   REGION          802..1062
FT                   /note="ATP-dependent helicase"
FT   BINDING         806..813
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1094 AA;  123427 MW;  A63871FF28889241 CRC64;
     MDSLSLPTVC DVVVPALNVD NLIRDYVSNV RADDSNNVSR FLGEVALKEI KSQVDTSNGD
     FQKLNVGFRL TPDEKNALKA NFPGLEIAFR DSCHSSHSFA AAHRVCETLN IYNRFKTKTE
     RIIDLGGNYV THAKQGRSNV HSCCPILDVR DGARHTDRYI SLAASVENRH RELPVDFCCH
     KFEECDVKAP FAMAIHSISD IPISTVATHC VRRGVRKLIA SVMMDPLMML YDKGHIPLLN
     VDWEKEDVEE TGKTLIHFHF VDAPGLSYSH DFDTLSQYMI TNQVIVNNSY SFRVERTACL
     SGVYIVEMTL SMTDGHSLAY LKPMRDVSCA WLSSLRKKVF VKLAVPISAE WYTEQFEVRH
     ALMDESLVRY VSEAAFRQFS KTKDPETLVQ YIATMLSSSS NHVVINGITM RSGSPIKFDE
     YVPLAVTFYV MAAWRYKMIA PGIDAVKTRT EKNVDILDEK GLIKEDFNVV NALLEDAGLI
     EPKLPHFTDV IKNAGLRGFG KKTIEKTRDD VLLIKPRSLL REVIYTVRSV FGLTILDSDY
     NLVSGVPSHM KATHVWSVFV GNLAFPSCLN VNECVNELLV NHMEMMEETK KEETRQQAFK
     DARDRALMTI AKAIEKDQTI KDGLLPILDL CKIKEELTAA SNSLSLTPEA IEQTDSRLMK
     ASGSDVNPYA DSIKEAIHYF NEVEVANTRN LRSLGIYLGW SIPKNKQTYD ALQGRNESVR
     VYVPYENKWY PSAPTSQYER AMTVDGYFSL PMEFEAITDG CRREISKYHL LVVDDSCIFC
     SGQRMIPALE AALKLVPTFK ITIVDGVAGC GKTTHLKKIA RIDSSAAGSP DLVLTSNRSS
     SDELKEVIDC PDVMKYRIRT VDSYLMLKSW FSAERLLFDE CFLTHAGCVY AAATLAQVKE
     VIAFGDTEQV PFISRLPEFR MEHHKVKGKI SCTTDHLQMS KRCHSLFKEN FLQKQDCEVS
     ERCSSLAELC PIQSVIQIQP ERDVLYMTHT RADKETLMRI PGMPKDRIKT THEAQGETWD
     HVVMFRLSKT TNLLHSGKGP DLGPCHNLVA ISRHRKSFRY FTVAPHDNDD QIVKCINYAR
     SLSSGDLDGV RVLI