CDF1_CAEEL
ID CDF1_CAEEL Reviewed; 561 AA.
AC Q95QW4; Q18009;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Cation diffusion facilitator family protein 1;
GN Name=cdf-1; ORFNames=C15B12.7;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RX PubMed=12015965; DOI=10.1016/s1534-5807(02)00151-x;
RA Bruinsma J.J., Jirakulaporn T., Muslin A.J., Kornfeld K.;
RT "Zinc ions and cation diffusion facilitator proteins regulate Ras-mediated
RT signaling.";
RL Dev. Cell 2:567-578(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP INTERACTION WITH LIN-45.
RX PubMed=15096503; DOI=10.1074/jbc.m401210200;
RA Jirakulaporn T., Muslin A.J.;
RT "Cation diffusion facilitator proteins modulate Raf-1 activity.";
RL J. Biol. Chem. 279:27807-27815(2004).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MUTAGENESIS OF
RP 199-GLU--GLU-561.
RX PubMed=23717214; DOI=10.1371/journal.pgen.1003522;
RA Roh H.C., Collier S., Deshmukh K., Guthrie J., Robertson J.D., Kornfeld K.;
RT "ttm-1 encodes CDF transporters that excrete zinc from intestinal cells of
RT C. elegans and act in a parallel negative feedback circuit that promotes
RT homeostasis.";
RL PLoS Genet. 9:E1003522-E1003522(2013).
CC -!- FUNCTION: Involved in the regulation of Pn.p cell fate determination
CC (PubMed:12015965). Involved in zinc metabolism and the decrease of the
CC cytosolic zinc concentration which is thought to modulate Ras signaling
CC (PubMed:12015965). Involved in zinc transport from the intestinal lumen
CC to the pseudocoelum (PubMed:23717214). {ECO:0000269|PubMed:12015965,
CC ECO:0000269|PubMed:23717214}.
CC -!- SUBUNIT: Interacts with lin-45 in a zinc-dependent manner.
CC {ECO:0000269|PubMed:15096503}.
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane
CC {ECO:0000269|PubMed:12015965, ECO:0000269|PubMed:23717214}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b {ECO:0000269|PubMed:9851916};
CC IsoId=Q95QW4-1; Sequence=Displayed;
CC Name=a {ECO:0000269|PubMed:12015965};
CC IsoId=Q95QW4-2; Sequence=VSP_052484;
CC -!- TISSUE SPECIFICITY: Expressed in intestinal cells (PubMed:12015965,
CC PubMed:23717214). Expressed in the vulva (PubMed:12015965).
CC {ECO:0000269|PubMed:12015965, ECO:0000269|PubMed:23717214}.
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. SLC30A subfamily. {ECO:0000255}.
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DR EMBL; FO080547; CCD64570.1; -; Genomic_DNA.
DR EMBL; FO080547; CCD64571.1; -; Genomic_DNA.
DR RefSeq; NP_509095.1; NM_076694.4. [Q95QW4-2]
DR RefSeq; NP_509096.1; NM_076695.3. [Q95QW4-1]
DR AlphaFoldDB; Q95QW4; -.
DR BioGRID; 45853; 2.
DR STRING; 6239.C15B12.7b; -.
DR TCDB; 2.A.4.2.4; the cation diffusion facilitator (cdf) family.
DR iPTMnet; Q95QW4; -.
DR EPD; Q95QW4; -.
DR PaxDb; Q95QW4; -.
DR PeptideAtlas; Q95QW4; -.
DR EnsemblMetazoa; C15B12.7a.1; C15B12.7a.1; WBGene00000393. [Q95QW4-2]
DR EnsemblMetazoa; C15B12.7a.2; C15B12.7a.2; WBGene00000393. [Q95QW4-2]
DR EnsemblMetazoa; C15B12.7b.1; C15B12.7b.1; WBGene00000393. [Q95QW4-1]
DR EnsemblMetazoa; C15B12.7b.2; C15B12.7b.2; WBGene00000393. [Q95QW4-1]
DR GeneID; 180923; -.
DR KEGG; cel:CELE_C15B12.7; -.
DR UCSC; C15B12.7b; c. elegans. [Q95QW4-1]
DR CTD; 180923; -.
DR WormBase; C15B12.7a; CE27065; WBGene00000393; cdf-1. [Q95QW4-2]
DR WormBase; C15B12.7b; CE29581; WBGene00000393; cdf-1. [Q95QW4-1]
DR eggNOG; KOG1483; Eukaryota.
DR GeneTree; ENSGT00940000171992; -.
DR InParanoid; Q95QW4; -.
DR OMA; IKIATRP; -.
DR OrthoDB; 820567at2759; -.
DR PhylomeDB; Q95QW4; -.
DR Reactome; R-CEL-425410; Metal ion SLC transporters.
DR Reactome; R-CEL-435368; Zinc efflux and compartmentalization by the SLC30 family.
DR SignaLink; Q95QW4; -.
DR PRO; PR:Q95QW4; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00000393; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR ExpressionAtlas; Q95QW4; baseline and differential.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:WormBase.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:WormBase.
DR GO; GO:0019901; F:protein kinase binding; IPI:WormBase.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; ISS:WormBase.
DR GO; GO:0071294; P:cellular response to zinc ion; IMP:WormBase.
DR GO; GO:0006882; P:cellular zinc ion homeostasis; IBA:GO_Central.
DR GO; GO:0010312; P:detoxification of zinc ion; IBA:GO_Central.
DR GO; GO:0046579; P:positive regulation of Ras protein signal transduction; IDA:WormBase.
DR GO; GO:0040026; P:positive regulation of vulval development; IGI:WormBase.
DR GO; GO:0071577; P:zinc ion transmembrane transport; ISS:WormBase.
DR Gene3D; 1.20.1510.10; -; 2.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR Pfam; PF01545; Cation_efflux; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Developmental protein; Ion transport;
KW Membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix;
KW Transport; Zinc; Zinc transport.
FT CHAIN 1..561
FT /note="Cation diffusion facilitator family protein 1"
FT /id="PRO_0000294932"
FT TOPO_DOM 1..112
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 134..136
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 158..176
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..215
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..391
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 413..420
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..441
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 442..561
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 240..304
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 246..270
FT /note="6 X 2 AA approximate repeats of H-G"
FT /evidence="ECO:0000255"
FT COMPBIAS 1..23
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 251..268
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 269..299
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 38..79
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000303|PubMed:12015965"
FT /id="VSP_052484"
FT MUTAGEN 199..561
FT /note="Missing: In n2527; loss of zinc transport from
FT intestinal cells to pseudocoelom."
FT /evidence="ECO:0000269|PubMed:23717214"
SQ SEQUENCE 561 AA; 61434 MW; 519038EE332B253A CRC64;
MEVTMEDRSV KADKADRDDN NTTSTELLGK MRQTKVISTN QNNNHLIDRM VSIYEMEMIK
SQSTETISDV SDVLEFTVID NCSHGTHTLE HDLRLKGEPI GKSESVKGVS RSLIIQIGMT
VIFCALEFIT GVVCSSIAML ADSYHMAADV MALIVAFTCI KIATRPSTRL GYGWVRAETL
GGFFNGIFMC TVCVLVFQEA VGRIINVHMI THPLQVLVIG FIGLLINLFG MFNLSGHGHS
HGGGSHGHSH GGSHGHSHNN KKTKKNDGHG HSHANGHGHS HDGKSDCNGE EEPDHTRLNG
KFRSASAMAN SDANVRLLDN DDNSNDIIER RLSGVNSQNT IIATVDRQMT PYGTHMASEV
LNVSSNNLDK SAQKTEQKKD KNVNIHGVWL HLLSDAFGSV IVMISAGFVY FLPTWKIAAY
LDPILSISLA SIMGFTAVVL VKTSGEKLLK QTPEGLDLEK VKKDLCSIVG VSKVEKLSVW
TLCGQRIIAA AHVNICHPAV FPEAAYKIKN YFHDLGVHST TIEPTFEDTC MQSMRIMVKK
VVDGKSIEEP VSVSTENEIT E
