CDF2_ARATH
ID CDF2_ARATH Reviewed; 457 AA.
AC Q93ZL5; Q5XF25; Q9FK95;
DT 09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 09-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Cyclic dof factor 2;
DE AltName: Full=Dof zinc finger protein DOF5.2;
DE Short=AtDOF5.2;
GN Name=CDF2; Synonyms=DOF5.2; OrderedLocusNames=At5g39660; ORFNames=MIJ24.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Shinn P., Chen H., Cheuk R., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12475498; DOI=10.1016/s1360-1385(02)02362-2;
RA Yanagisawa S.;
RT "The Dof family of plant transcription factors.";
RL Trends Plant Sci. 7:555-560(2002).
RN [6]
RP INTERACTION WITH ADO2 AND ADO3.
RX PubMed=16002617; DOI=10.1126/science.1110586;
RA Imaizumi T., Schultz T.F., Harmon F.G., Ho L.A., Kay S.A.;
RT "FKF1 F-box protein mediates cyclic degradation of a repressor of CONSTANS
RT in Arabidopsis.";
RL Science 309:293-297(2005).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19619493; DOI=10.1016/j.devcel.2009.06.015;
RA Fornara F., Panigrahi K.C., Gissot L., Sauerbrunn N., Ruehl M.,
RA Jarillo J.A., Coupland G.;
RT "Arabidopsis DOF transcription factors act redundantly to reduce CONSTANS
RT expression and are essential for a photoperiodic flowering response.";
RL Dev. Cell 17:75-86(2009).
CC -!- FUNCTION: Transcription factor that binds specifically to a 5'-AA[AG]G-
CC 3' consensus core sequence (By similarity). Regulates a photoperiodic
CC flowering response. Transcriptional repressor of 'CONSTANS' expression.
CC The stability of CDF2 is controlled by 'GIGANTEA' and redundantly by
CC ADO3, ADO2 and/or ADO1. {ECO:0000250, ECO:0000269|PubMed:19619493}.
CC -!- SUBUNIT: Interacts with ADO2 (via kelch repeats) and ADO3 (via kelch
CC repeats). {ECO:0000269|PubMed:16002617}.
CC -!- INTERACTION:
CC Q93ZL5; Q8W420: ADO2; NbExp=3; IntAct=EBI-1536103, EBI-1015688;
CC Q93ZL5; Q9C9W9: ADO3; NbExp=2; IntAct=EBI-1536103, EBI-401228;
CC Q93ZL5; O64647: TCP9; NbExp=3; IntAct=EBI-1536103, EBI-9838721;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the vasculature of cotyledons and
CC hypocotyls, leaves and roots. {ECO:0000269|PubMed:19619493}.
CC -!- INDUCTION: Circadian-regulation. Highly expressed at the beginning of
CC the light period, then decreases, reaching a minimum between 16 and 29
CC hours after dawn before rising again at the end of the day. Regulated
CC at the protein level by ADO3 and GI pos-transcriptionally.
CC {ECO:0000269|PubMed:19619493}.
CC -!- DISRUPTION PHENOTYPE: Early flowering. {ECO:0000269|PubMed:19619493}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08898.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB012243; BAB08898.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED94460.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94461.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69712.1; -; Genomic_DNA.
DR EMBL; AY056804; AAL10495.1; -; mRNA.
DR EMBL; BT015791; AAU90081.1; -; mRNA.
DR RefSeq; NP_001318708.1; NM_001344299.1.
DR RefSeq; NP_568567.1; NM_123328.2.
DR RefSeq; NP_851106.1; NM_180775.3.
DR AlphaFoldDB; Q93ZL5; -.
DR BioGRID; 19213; 16.
DR IntAct; Q93ZL5; 15.
DR STRING; 3702.AT5G39660.1; -.
DR PaxDb; Q93ZL5; -.
DR PRIDE; Q93ZL5; -.
DR ProteomicsDB; 223969; -.
DR EnsemblPlants; AT5G39660.1; AT5G39660.1; AT5G39660.
DR EnsemblPlants; AT5G39660.2; AT5G39660.2; AT5G39660.
DR EnsemblPlants; AT5G39660.3; AT5G39660.3; AT5G39660.
DR GeneID; 833962; -.
DR Gramene; AT5G39660.1; AT5G39660.1; AT5G39660.
DR Gramene; AT5G39660.2; AT5G39660.2; AT5G39660.
DR Gramene; AT5G39660.3; AT5G39660.3; AT5G39660.
DR KEGG; ath:AT5G39660; -.
DR Araport; AT5G39660; -.
DR TAIR; locus:2164860; AT5G39660.
DR eggNOG; ENOG502QSI8; Eukaryota.
DR HOGENOM; CLU_030533_1_0_1; -.
DR InParanoid; Q93ZL5; -.
DR OMA; PAASYWG; -.
DR OrthoDB; 853832at2759; -.
DR PhylomeDB; Q93ZL5; -.
DR PRO; PR:Q93ZL5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q93ZL5; baseline and differential.
DR Genevisible; Q93ZL5; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR InterPro; IPR045174; Dof.
DR InterPro; IPR003851; Znf_Dof.
DR PANTHER; PTHR31089; PTHR31089; 1.
DR Pfam; PF02701; zf-Dof; 1.
DR PROSITE; PS01361; ZF_DOF_1; 1.
DR PROSITE; PS50884; ZF_DOF_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Flowering; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..457
FT /note="Cyclic dof factor 2"
FT /id="PRO_0000074292"
FT ZN_FING 138..192
FT /note="Dof-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT REGION 1..130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 334..377
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 417..457
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..41
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 59..91
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..124
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 334..351
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..377
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 140
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 143
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 165
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 168
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT CONFLICT 277
FT /note="G -> R (in Ref. 3; AAL10495)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 457 AA; 49808 MW; 8D95915A1AF8B945 CRC64;
MADPAIKLFG KTIPLPELGV VDSSSSYTGF LTETQIPVRL SDSCTGDDDD EEMGDSGLGR
EEGDDVGDGG GESETDKKEE KDSECQEESL RNESNDVTTT TSGITEKTET TKAAKTNEES
GGTACSQEGK LKKPDKILPC PRCNSMETKF CYYNNYNVNQ PRHFCKKCQR YWTAGGTMRN
VPVGAGRRKN KSPASHYNRH VSITSAEAMQ KVARTDLQHP NGANLLTFGS DSVLCESMAS
GLNLVEKSLL KTQTVLQEPN EGLKITVPLN QTNEEAGTVS PLPKVPCFPG PPPTWPYAWN
GVSWTILPFY PPPAYWSCPG VSPGAWNSFT WMPQPNSPSG SNPNSPTLGK HSRDENAAEP
GTAFDETESL GREKSKPERC LWVPKTLRID DPEEAAKSSI WETLGIKKDE NADTFGAFRS
STKEKSSLSE GRLPGRRPEL QANPAALSRS ANFHESS
