CDF3_ARATH
ID CDF3_ARATH Reviewed; 448 AA.
AC Q8LFV3; O81600;
DT 25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Cyclic dof factor 3;
DE AltName: Full=Dof zinc finger protein DOF3.3;
DE Short=AtDOF3.3;
DE AltName: Full=H-protein promoter-binding factor 2a;
GN Name=CDF3; Synonyms=DOF3.3, HPPBF-2A; OrderedLocusNames=At3g47500;
GN ORFNames=F1P2.50;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Abbaraju H.K.R., Oliver D.J.;
RT "Molecular cloning of the H-protein promoter binding factor from
RT Arabidopsis.";
RL Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12475498; DOI=10.1016/s1360-1385(02)02362-2;
RA Yanagisawa S.;
RT "The Dof family of plant transcription factors.";
RL Trends Plant Sci. 7:555-560(2002).
RN [7]
RP INTERACTION WITH ADO2 AND ADO3.
RX PubMed=16002617; DOI=10.1126/science.1110586;
RA Imaizumi T., Schultz T.F., Harmon F.G., Ho L.A., Kay S.A.;
RT "FKF1 F-box protein mediates cyclic degradation of a repressor of CONSTANS
RT in Arabidopsis.";
RL Science 309:293-297(2005).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19619493; DOI=10.1016/j.devcel.2009.06.015;
RA Fornara F., Panigrahi K.C., Gissot L., Sauerbrunn N., Ruehl M.,
RA Jarillo J.A., Coupland G.;
RT "Arabidopsis DOF transcription factors act redundantly to reduce CONSTANS
RT expression and are essential for a photoperiodic flowering response.";
RL Dev. Cell 17:75-86(2009).
CC -!- FUNCTION: Transcription factor that binds specifically to a 5'-AA[AG]G-
CC 3' consensus core sequence (By similarity). Regulates a photoperiodic
CC flowering response. Transcriptional repressor of 'CONSTANS' expression.
CC {ECO:0000250, ECO:0000269|PubMed:19619493}.
CC -!- SUBUNIT: Interacts with ADO2 (via kelch repeats) and ADO3 (via kelch
CC repeats). {ECO:0000269|PubMed:16002617}.
CC -!- INTERACTION:
CC Q8LFV3; Q8W420: ADO2; NbExp=3; IntAct=EBI-1536119, EBI-1015688;
CC Q8LFV3; Q9C9W9: ADO3; NbExp=2; IntAct=EBI-1536119, EBI-401228;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the vasculature of cotyledons and
CC hypocotyls, leaves and roots. {ECO:0000269|PubMed:19619493}.
CC -!- INDUCTION: Circadian-regulation. Highly expressed at the beginning of
CC the light period, then decreases, reaching a minimum between 16 and 29
CC hours after dawn before rising again at the end of the day.
CC {ECO:0000269|PubMed:19619493}.
CC -!- DISRUPTION PHENOTYPE: No alteration in flowering time, probably due to
CC the redundancy with CDF1, CDF2 and CDF5. {ECO:0000269|PubMed:19619493}.
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DR EMBL; AF079503; AAC28390.1; -; mRNA.
DR EMBL; AL132955; CAB61976.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78290.1; -; Genomic_DNA.
DR EMBL; AY065152; AAL38328.1; -; mRNA.
DR EMBL; AY128757; AAM91157.1; -; mRNA.
DR EMBL; AY084628; AAM61191.1; -; mRNA.
DR PIR; T45710; T45710.
DR RefSeq; NP_190334.1; NM_114618.4.
DR AlphaFoldDB; Q8LFV3; -.
DR BioGRID; 9224; 2.
DR IntAct; Q8LFV3; 10.
DR STRING; 3702.AT3G47500.1; -.
DR iPTMnet; Q8LFV3; -.
DR PaxDb; Q8LFV3; -.
DR PRIDE; Q8LFV3; -.
DR ProteomicsDB; 220465; -.
DR EnsemblPlants; AT3G47500.1; AT3G47500.1; AT3G47500.
DR GeneID; 823904; -.
DR Gramene; AT3G47500.1; AT3G47500.1; AT3G47500.
DR KEGG; ath:AT3G47500; -.
DR Araport; AT3G47500; -.
DR TAIR; locus:2079152; AT3G47500.
DR eggNOG; ENOG502QSI8; Eukaryota.
DR HOGENOM; CLU_030533_1_0_1; -.
DR InParanoid; Q8LFV3; -.
DR OMA; HHIMISE; -.
DR OrthoDB; 853832at2759; -.
DR PhylomeDB; Q8LFV3; -.
DR PRO; PR:Q8LFV3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8LFV3; baseline and differential.
DR Genevisible; Q8LFV3; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR InterPro; IPR045174; Dof.
DR InterPro; IPR003851; Znf_Dof.
DR PANTHER; PTHR31089; PTHR31089; 1.
DR Pfam; PF02701; zf-Dof; 1.
DR PROSITE; PS01361; ZF_DOF_1; 1.
DR PROSITE; PS50884; ZF_DOF_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Flowering; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..448
FT /note="Cyclic dof factor 3"
FT /id="PRO_0000074279"
FT ZN_FING 110..164
FT /note="Dof-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT REGION 26..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 243..269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 332..370
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 51..68
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 83..102
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 245..269
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 332..351
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..370
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 112
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 115
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 137
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT BINDING 140
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT CONFLICT 88
FT /note="D -> E (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 201
FT /note="Q -> H (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 206
FT /note="Missing (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 210
FT /note="T -> A (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 217
FT /note="E -> G (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 228
FT /note="R -> G (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 270..271
FT /note="SV -> RI (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 274
FT /note="A -> P (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 277..278
FT /note="Missing (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 327..328
FT /note="LP -> ISPS (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 340
FT /note="C -> D (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 351..352
FT /note="HP -> RS (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 356
FT /note="G -> E (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 360..362
FT /note="KDN -> RDS (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 369..370
FT /note="KA -> RT (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
FT CONFLICT 373
FT /note="V -> I (in Ref. 5; AAM61191)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 448 AA; 49730 MW; 6E61FA7A7A8DF1F6 CRC64;
MMMETRDPAI KLFGMKIPFP SVFESAVTVE DDEEDDWSGG DDKSPEKVTP ELSDKNNNNC
NDNSFNNSKP ETLDKEEATS TDQIESSDTP EDNQQTTPDG KTLKKPTKIL PCPRCKSMET
KFCYYNNYNI NQPRHFCKAC QRYWTAGGTM RNVPVGAGRR KNKSSSSHYR HITISEALEA
ARLDPGLQAN TRVLSFGLEA QQQHVAAPMT PVMKLQEDQK VSNGARNRFH GLADQRLVAR
VENGDDCSSG SSVTTSNNHS VDESRAQSGS VVEAQMNNNN NNNMNGYACI PGVPWPYTWN
PAMPPPGFYP PPGYPMPFYP YWTIPMLPPH QSSSPISQKC SNTNSPTLGK HPRDEGSSKK
DNETERKQKA GCVLVPKTLR IDDPNEAAKS SIWTTLGIKN EAMCKAGGMF KGFDHKTKMY
NNDKAENSPV LSANPAALSR SHNFHEQI
