CDGAP_DROME
ID CDGAP_DROME Reviewed; 1843 AA.
AC Q9VIS1; Q6AWI4; Q8MSV9; Q9NCL1;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=GTPase-activating protein CdGAPr;
DE AltName: Full=d-CdGAPr;
GN Name=CdGAPr; ORFNames=CG10538;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=10842085; DOI=10.1016/s0925-4773(00)00291-4;
RA Sagnier T., Grienenberger A., Mariol M.-C., Berenger H., Pradel J.,
RA Graba Y.;
RT "Dynamic expression of d-CdGAPr, a novel Drosophila melanogaster gene
RT encoding a GTPase activating protein.";
RL Mech. Dev. 94:267-270(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1062-1843.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP FUNCTION.
RX PubMed=17360775; DOI=10.1242/dev.001529;
RA Murakami S., Umetsu D., Maeyama Y., Sato M., Yoshida S., Tabata T.;
RT "Focal adhesion kinase controls morphogenesis of the Drosophila optic
RT stalk.";
RL Development 134:1539-1548(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1216, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166; SER-168; SER-180;
RP SER-837; SER-840; THR-843; SER-851; SER-861; SER-872; SER-876; SER-1029;
RP THR-1118; THR-1121; SER-1125; SER-1281; SER-1284; SER-1453; SER-1456 AND
RP THR-1679, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Probably functions as a GTPase-activating protein (GAP) for
CC RAC1 and/or CDC42. Required for optic stalk formation.
CC {ECO:0000269|PubMed:17360775}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:10842085}.
CC -!- DEVELOPMENTAL STAGE: Expressed at all developmental stages, with
CC highest expression at the posterior pole of the early cellular
CC blastoderm, the neuro-ectoderm prior to neuroblast delamination, rows
CC of epidermal cells in the most posterior part of thoracic and first
CC abdominal segments and a ring of epidermal cells at the posterior end
CC of the embryo. {ECO:0000269|PubMed:10842085}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM49910.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAT94493.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF218776; AAF44627.1; -; mRNA.
DR EMBL; AE014134; AAF53844.2; -; Genomic_DNA.
DR EMBL; BT015264; AAT94493.1; ALT_FRAME; mRNA.
DR EMBL; AY118541; AAM49910.1; ALT_INIT; mRNA.
DR RefSeq; NP_001260600.1; NM_001273671.2.
DR RefSeq; NP_610002.1; NM_136158.5.
DR AlphaFoldDB; Q9VIS1; -.
DR SMR; Q9VIS1; -.
DR BioGRID; 61243; 3.
DR IntAct; Q9VIS1; 3.
DR STRING; 7227.FBpp0304488; -.
DR iPTMnet; Q9VIS1; -.
DR PaxDb; Q9VIS1; -.
DR PRIDE; Q9VIS1; -.
DR DNASU; 35267; -.
DR EnsemblMetazoa; FBtr0081318; FBpp0080851; FBgn0032821.
DR EnsemblMetazoa; FBtr0332179; FBpp0304488; FBgn0032821.
DR GeneID; 35267; -.
DR KEGG; dme:Dmel_CG10538; -.
DR UCSC; CG10538-RA; d. melanogaster.
DR CTD; 35267; -.
DR FlyBase; FBgn0032821; CdGAPr.
DR VEuPathDB; VectorBase:FBgn0032821; -.
DR eggNOG; KOG1449; Eukaryota.
DR GeneTree; ENSGT00940000168991; -.
DR InParanoid; Q9VIS1; -.
DR OMA; FERGQPM; -.
DR OrthoDB; 17584at2759; -.
DR PhylomeDB; Q9VIS1; -.
DR Reactome; R-DME-8980692; RHOA GTPase cycle.
DR Reactome; R-DME-9013026; RHOB GTPase cycle.
DR Reactome; R-DME-9013106; RHOC GTPase cycle.
DR Reactome; R-DME-9013148; CDC42 GTPase cycle.
DR Reactome; R-DME-9013149; RAC1 GTPase cycle.
DR Reactome; R-DME-9013405; RHOD GTPase cycle.
DR Reactome; R-DME-9013420; RHOU GTPase cycle.
DR Reactome; R-DME-9035034; RHOF GTPase cycle.
DR SignaLink; Q9VIS1; -.
DR BioGRID-ORCS; 35267; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 35267; -.
DR PRO; PR:Q9VIS1; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032821; Expressed in parasegment (Drosophila) and 33 other tissues.
DR ExpressionAtlas; Q9VIS1; baseline and differential.
DR Genevisible; Q9VIS1; DM.
DR GO; GO:0005096; F:GTPase activator activity; ISS:FlyBase.
DR GO; GO:0031290; P:retinal ganglion cell axon guidance; IMP:UniProtKB.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IBA:GO_Central.
DR Gene3D; 1.10.555.10; -; 1.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SMART; SM00326; SH3; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR SUPFAM; SSF50044; SSF50044; 1.
DR PROSITE; PS50238; RHOGAP; 1.
DR PROSITE; PS50002; SH3; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Developmental protein; GTPase activation; Phosphoprotein;
KW Reference proteome; SH3 domain.
FT CHAIN 1..1843
FT /note="GTPase-activating protein CdGAPr"
FT /id="PRO_0000320117"
FT DOMAIN 295..363
FT /note="SH3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT DOMAIN 424..618
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 94..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 746..780
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 894..975
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1013..1106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1721..1745
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1779..1829
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1378..1404
FT /evidence="ECO:0000255"
FT COMPBIAS 760..780
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 926..952
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1027..1052
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1068..1099
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1721..1744
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1779..1794
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1803..1829
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 166
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 168
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 180
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 837
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 840
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 843
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 851
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 861
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 872
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 876
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1029
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1118
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1121
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1125
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1216
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT MOD_RES 1281
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1284
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1453
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1456
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1679
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 1092
FT /note="Missing (in Ref. 4; AAT94493 and 5; AAM49910)"
FT /evidence="ECO:0000305"
FT CONFLICT 1818..1820
FT /note="NNR -> K (in Ref. 5; AAM49910)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1843 AA; 204217 MW; 4D5C65C637765D57 CRC64;
MSDKVSTSLA AQLHSHALAS AEAGGIGGVN GGPIACHAST DKDAQAPPLQ FEMDAPAAAI
SAQLNFKIVP ADSLPGLSYQ DMFASMNTSQ ISNASTESSC SPPIQGAVPP PKPSRHMAVQ
PLNSKSCRFP KLEECAHFHY ERVQLGPLSV QLLDDKSEHM GSSIASQSLG GDLPHSSLRS
FSPESCWFII RVCPQRCEPF LIKRSFENMQ LLDEMLHRCV YDRKISGLRN MEELAAELPS
ESDVEYAVAK YLERFSKIAS DSLTCGTILT WLQLDNKGRR LPLADGETQR TINTPAVGAA
YGVRRYQAQA PDEINIEVGD MISVIDMPSP AESIWWRGKK SHLQKSLYEV GFFPQSCVAT
IGDKVPRNFP MPAPLVGHLD ASPTKPVLRK HGKLIAFFRS FILSRPSRRR LKQSGIYRER
VFNCDLSEHL LNSGQDIPMV LRSCAEFIEN YGVIDGIYRL SGITSNIQRL RRAFDEERVP
DLGNPEMKQD IHAVSSLLKM YFRELPNPLC TYQLYDNFVE AIQVKADEAD ERLRLMKETV
LKLPPPHYRT LKYLAEHLYK VSQHHGRTGM TDKNLAIVWA PNLLRSPALE SGGVAALRGV
GVQAVVTEYL IRNCHNIFDA LDDHPARHSM VASATAAAAN AAGGELRLES LTDCESLLVE
QREQDQSLGV VERPKSLSTG GAKLISLEEA QERHSRVEGA DLKQSLPISM LTSASSNAAS
NIGSYIEVGG GPSSLPDKYH TVLSAPRSWQ KRKPDKTPSW KSIFSRSQRQ GNPDPGQKIT
TDVKDSVASR VSFVQASHAH ASKELTKHDK PKSIELLETT SNERDPKPMD LCIRSNSIDS
LRTVGHSRSV SHDSYFDLLQ SPQRGHMTTC PSRELSELGL NFDREEPEMR IFSESESLVS
SPRVGKENVP PASGSATRRI MRARPEDFSS QTNSVNPSPK KQPRLNLLSP SSARTMPPPP
LSAPGTASSS CGHESGGAEN CCKRYKLEDQ LCDIQFIDCG TPENVPTTQQ QFASVEVHPP
PKPARANQSP ISSSNGASVG SSSSSTRYSY PSVQLGAKRK EQQDAKERFS YQGTFTQPGQ
KQESSAPRPV HSTNNGATLR KPRVEPVEDG QIKLHVPTPT TPARSPRYSL LLCDTESSDN
SSAVNTPQYD MEPLMLTSAM SGVSGVSSNV QQQQLLLGVD ASSSYLGSSH ESLGQNINNH
HDAEQRDMNA LKRELSLDLN PLQPRLPQPA NRAATLPVKD QLQAAAAAMC SSPNNSNFTD
NTSQSVTPSE YGYQHLQRQL SMHSLLATDE SSPVYEDFEQ TPVKMVPSPI KSTISITYKS
PEKEKKPSAI LETNFDENTV YEQVKLFRNS VTEVNQMLHE RSSLKQIAEE EQHDGGAYKA
AEMTQQLLQL EQEHHDHEEQ QEKEPEDEEQ SQLLYENIEL RKPKTVYENL RGEEMKFITE
EQKPNVDRIE LDSLDSLPDN MEHEQSSPSK SPTFSVKELA NKFESSPVEQ LPNFDFSVRG
GSMKKPNELS VPKTMPAPVP LKKLNSSAQK ITRSLDENAF VREFGGKQLQ DLSLSNKLPE
VMSEVNSRRK SFDFTRPKTL NPPKRLPGMS ITEEICQKRV GEMEAITPTT ENRISLIQQN
NMPKEQQSSA NQFLPPAGRK NVLTGVVLDR ERIDKIKEER RQQLTQKYYG DTLKSRSRTE
LNSEDNFPAA ESLRIKSKSR GDMHALQKDI DMNLKQLSRV TGGGSECTLH TGLSQGNGQE
HLGQQRVRRI SDEKNQNCDT SNGGVSGITS TSLLSVKTTA QKYGSTSKTP ANKFERSQPM
PRDRLQRNSL AESNAGTNNR EKISPQFSIR DVTAMFESRS QNQ
