CDK14_XENTR
ID CDK14_XENTR Reviewed; 423 AA.
AC A4IIW7;
DT 02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Cyclin-dependent kinase 14;
DE EC=2.7.11.22;
DE AltName: Full=Cell division protein kinase 14;
GN Name=cdk14; Synonyms=pftk1;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Serine/threonine-protein kinase involved in the control of
CC the eukaryotic cell cycle, whose activity is controlled by an
CC associated cyclin. Acts as a cell-cycle regulator of Wnt signaling
CC pathway during G2/M phase by mediating the phosphorylation of lrp6,
CC leading to the activation of the Wnt signaling pathway (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.22;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.22;
CC -!- SUBUNIT: Interacts with ccny; ccny mediates its recruitment to the
CC plasma membrane and promotes phosphorylation of lrp6. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}. Note=Recruited to the cell membrane by CCNY.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr
CC protein kinase family. CDC2/CDKX subfamily. {ECO:0000305}.
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DR EMBL; BC136183; AAI36184.1; -; mRNA.
DR RefSeq; NP_001096458.1; NM_001102988.1.
DR AlphaFoldDB; A4IIW7; -.
DR SMR; A4IIW7; -.
DR PaxDb; A4IIW7; -.
DR DNASU; 100125075; -.
DR Ensembl; ENSXETT00000007502; ENSXETP00000007502; ENSXETG00000003466.
DR GeneID; 100125075; -.
DR KEGG; xtr:100125075; -.
DR CTD; 5218; -.
DR Xenbase; XB-GENE-943250; cdk14.
DR eggNOG; KOG0594; Eukaryota.
DR HOGENOM; CLU_000288_181_6_1; -.
DR InParanoid; A4IIW7; -.
DR OrthoDB; 1010560at2759; -.
DR Proteomes; UP000008143; Chromosome 6.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000003466; Expressed in brain and 9 other tissues.
DR ExpressionAtlas; A4IIW7; baseline.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0000308; C:cytoplasmic cyclin-dependent protein kinase holoenzyme complex; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0030332; F:cyclin binding; IBA:GO_Central.
DR GO; GO:0004693; F:cyclin-dependent protein serine/threonine kinase activity; ISS:UniProtKB.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000086; P:G2/M transition of mitotic cell cycle; ISS:UniProtKB.
DR GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR GO; GO:0060828; P:regulation of canonical Wnt signaling pathway; ISS:UniProtKB.
DR GO; GO:0000083; P:regulation of transcription involved in G1/S transition of mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cell cycle; Cell division; Cell membrane; Kinase; Membrane;
KW Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase;
KW Transferase; Wnt signaling pathway.
FT CHAIN 1..423
FT /note="Cyclin-dependent kinase 14"
FT /id="PRO_0000391904"
FT DOMAIN 89..373
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 62..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 210
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 95..103
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 118
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 423 AA; 47680 MW; 4D229A5E1558FDB5 CRC64;
MSTRNCQGID SVIKPLDTIP EDKKVRVQRT QSSFDPFEKT SNQVKRVHSE NNACINFKSA
SVGKESPKVR RHSSPSSPTS PKFGKADSYE KLEKLGEGSY ATVYKGKSKV NGKLVALKVI
RLQEEEGTPF TAIREASLLK GLKHANIVLL HDIIHTKETL TLVFEYVHTD LCQYMDKHPG
GLNPENVKLF LFQLLRGLSY IHQGHILHRD LKPQNLLISD TGELKLADFG LARAKSVPSH
TYSNEVVTLW YRPPDVLLGS TDYSTCLDMW GVGCIFVEMI QGVAAFPGMK DIQDQLERIF
LILGTPNEET WPGVHSLPHF KLERFTQYGP KNLRQAWNKL SYVNHAEDLA SKLLQCFPKN
RLSAQAALNH DYFSDLPPRL WELSDMSSIF TVPNVKLQPE AGESMRVFGK NNSFSKSLSN
SKH
