CDK7_CAEEL
ID CDK7_CAEEL Reviewed; 330 AA.
AC G5EFV5;
DT 01-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Cyclin-dependent kinase 7 {ECO:0000305};
DE EC=2.7.11.22 {ECO:0000250|UniProtKB:P50613};
DE EC=2.7.11.23 {ECO:0000269|PubMed:11960010};
DE AltName: Full=Cell division protein kinase 7 {ECO:0000305};
GN Name=cdk-7 {ECO:0000312|WormBase:Y39G10AL.3};
GN ORFNames=Y39G10AL.3 {ECO:0000312|WormBase:Y39G10AL.3};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AAD38186.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:AAD38186.1};
RA Liu J., Kipreos E.T.;
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP CYS-280.
RX PubMed=11960010; DOI=10.1073/pnas.082618399;
RA Wallenfang M.R., Seydoux G.;
RT "cdk-7 is required for mRNA transcription and cell cycle progression in
RT Caenorhabditis elegans embryos.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:5527-5532(2002).
CC -!- FUNCTION: Serine/threonine kinase involved in cell cycle control and in
CC RNA polymerase II-mediated RNA transcription. Required for maintaining
CC chromosome ploidy. May phosphorylate the large subunit of RNA
CC polymerase II, ama-1. {ECO:0000269|PubMed:11960010}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.22;
CC Evidence={ECO:0000250|UniProtKB:P50613};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.22; Evidence={ECO:0000250|UniProtKB:P50613};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[DNA-directed RNA polymerase] + ATP = ADP + H(+) + phospho-
CC [DNA-directed RNA polymerase]; Xref=Rhea:RHEA:10216, Rhea:RHEA-
CC COMP:11321, Rhea:RHEA-COMP:11322, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43176, ChEBI:CHEBI:68546,
CC ChEBI:CHEBI:456216; EC=2.7.11.23;
CC Evidence={ECO:0000269|PubMed:11960010};
CC -!- SUBUNIT: Catalytic component which, in association with cyclin H (cyh-
CC 1) and mat1, is likely to form the CAK complex.
CC {ECO:0000250|UniProtKB:P50613}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in an arrest at
CC approximately 50-cell embryonic stage. The phenotype is more severe
CC when done in a cdk7(ax224) mutant background where the embryonic arrest
CC occurs at the 1-cell stage due to an arrest in early meiosis.
CC {ECO:0000269|PubMed:11960010}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr
CC protein kinase family. CDC2/CDKX subfamily. {ECO:0000305}.
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DR EMBL; AF154004; AAD38186.1; -; mRNA.
DR EMBL; FO081124; CCD69307.1; -; Genomic_DNA.
DR RefSeq; NP_490952.2; NM_058551.6.
DR AlphaFoldDB; G5EFV5; -.
DR SMR; G5EFV5; -.
DR STRING; 6239.Y39G10AL.3.1; -.
DR EPD; G5EFV5; -.
DR PaxDb; G5EFV5; -.
DR PeptideAtlas; G5EFV5; -.
DR EnsemblMetazoa; Y39G10AL.3.1; Y39G10AL.3.1; WBGene00000408.
DR EnsemblMetazoa; Y39G10AL.3.2; Y39G10AL.3.2; WBGene00000408.
DR EnsemblMetazoa; Y39G10AL.3.3; Y39G10AL.3.3; WBGene00000408.
DR GeneID; 171784; -.
DR CTD; 171784; -.
DR WormBase; Y39G10AL.3; CE30320; WBGene00000408; cdk-7.
DR eggNOG; KOG0659; Eukaryota.
DR GeneTree; ENSGT00940000155179; -.
DR HOGENOM; CLU_000288_181_1_1; -.
DR InParanoid; G5EFV5; -.
DR OMA; GIHHCHR; -.
DR OrthoDB; 1367115at2759; -.
DR PhylomeDB; G5EFV5; -.
DR Reactome; R-CEL-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-CEL-113418; Formation of the Early Elongation Complex.
DR Reactome; R-CEL-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-CEL-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-CEL-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-CEL-6782135; Dual incision in TC-NER.
DR Reactome; R-CEL-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-CEL-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-CEL-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-CEL-69202; Cyclin E associated events during G1/S transition.
DR Reactome; R-CEL-69273; Cyclin A/B1/B2 associated events during G2/M transition.
DR Reactome; R-CEL-69656; Cyclin A:Cdk2-associated events at S phase entry.
DR Reactome; R-CEL-72086; mRNA Capping.
DR Reactome; R-CEL-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-CEL-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-CEL-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-CEL-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-CEL-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-CEL-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-CEL-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR PRO; PR:G5EFV5; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00000408; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; ISS:WormBase.
DR GO; GO:0070985; C:transcription factor TFIIK complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004693; F:cyclin-dependent protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0008353; F:RNA polymerase II CTD heptapeptide repeat kinase activity; ISS:WormBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0045448; P:mitotic cell cycle, embryonic; IMP:WormBase.
DR GO; GO:0009299; P:mRNA transcription; IDA:WormBase.
DR GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; ISS:WormBase.
DR CDD; cd07841; STKc_CDK7; 1.
DR InterPro; IPR037770; CDK7.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR PANTHER; PTHR24056:SF375; PTHR24056:SF375; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell cycle; Cell division; Kinase; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Serine/threonine-protein kinase;
KW Transcription; Transcription regulation; Transferase.
FT CHAIN 1..330
FT /note="Cyclin-dependent kinase 7"
FT /id="PRO_0000432621"
FT DOMAIN 5..289
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 305..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 130
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 11..19
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 34
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 163
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P50613"
FT MUTAGEN 280
FT /note="C->T: In ax224; temperature-sensitive embryonic
FT arrest at approximately 50-cell stage characterized by a
FT severe defect in the transcription of several mRNAs and a
FT severe decrease in ama-1 phosphorylation. Increase in cell
FT cycle length at the interphase and M phase stages."
FT /evidence="ECO:0000269|PubMed:11960010"
SQ SEQUENCE 330 AA; 38353 MW; D036FF760565287B CRC64;
MSRRYDTIKH LGEGQFANVY LAQDLESGEC VAIKKIKLGS REEAKDGINR TAIREIKLLK
EIHHDNIIGL RDVIGHRTSI QLVFDFMDTD LEHVIKDKEI ILMPAHIKNI TMQMLLGLEF
LHVHWILHRD LKPNNLLMNK MGRVKLTDFG LARFFGSPNR NYTHQVVTRW YRAPELLFGA
RSYGVGIDIW SVGCIIAELL LRNPIFPGES DIDQLVKIFN ILGCPTPETW PNMTEMNSYV
IIKPQTEYMA LNYYFSAAPQ DLLDLMAGMW TFDPIKRLTC TQSLQMEYFR TQPFCCLDEE
LPLPKKQQPQ KRSRRLDDDG TRPVRRLNFD
