CDKA1_MOUSE
ID CDKA1_MOUSE Reviewed; 114 AA.
AC O35207; B2RV53; Q3SYK3; Q6ZWZ1;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Cyclin-dependent kinase 2-associated protein 1;
DE Short=CDK2-associated protein 1;
DE AltName: Full=Deleted in oral cancer 1;
DE Short=DOC-1;
DE AltName: Full=Putative oral cancer suppressor;
GN Name=Cdk2ap1; Synonyms=Cdkap1, Doc1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N-3; TISSUE=Brain, Colon, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 6-114.
RC TISSUE=Fibroblast;
RA Wong D.T.W., Tsuji T., Todd R., McBride J.;
RT "Molecular cloning and characterization of mouse doc-1 oral tumor
RT suppressor gene.";
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND INTERACTION WITH CDK2.
RX PubMed=10938106; DOI=10.1128/mcb.20.17.6300-6307.2000;
RA Shintani S., Ohyama H., Zhang X., McBride J., Matsuo K., Tsuji T., Hu M.G.,
RA Hu G., Kohno Y., Lerman M., Todd R., Wong D.T.;
RT "p12(DOC-1) is a novel cyclin-dependent kinase 2-associated protein.";
RL Mol. Cell. Biol. 20:6300-6307(2000).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: specific inhibitor of the cell-cycle kinase CDK2.
CC {ECO:0000269|PubMed:10938106}.
CC -!- SUBUNIT: Homodimer (By similarity). Interacts with monomeric
CC unphosphorylated CDK2 (PubMed:10938106). Interacts with CDK2AP2 (By
CC similarity). {ECO:0000250|UniProtKB:O14519,
CC ECO:0000269|PubMed:10938106}.
CC -!- PTM: Phosphorylated in vitro by IKBKE at Ser-45. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CDK2AP family. {ECO:0000305}.
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DR EMBL; AK004852; BAB23616.1; -; mRNA.
DR EMBL; AL645849; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC083075; AAH83075.1; -; mRNA.
DR EMBL; BC103772; AAI03773.1; -; mRNA.
DR EMBL; BC147052; AAI47053.1; -; mRNA.
DR EMBL; BC147053; AAI47054.1; -; mRNA.
DR EMBL; AF011644; AAB65752.1; -; mRNA.
DR CCDS; CCDS39279.1; -.
DR RefSeq; NP_038840.2; NM_013812.2.
DR AlphaFoldDB; O35207; -.
DR SMR; O35207; -.
DR BioGRID; 199264; 14.
DR IntAct; O35207; 13.
DR STRING; 10090.ENSMUSP00000031341; -.
DR PhosphoSitePlus; O35207; -.
DR EPD; O35207; -.
DR MaxQB; O35207; -.
DR PaxDb; O35207; -.
DR PeptideAtlas; O35207; -.
DR PRIDE; O35207; -.
DR ProteomicsDB; 281441; -.
DR DNASU; 13445; -.
DR Ensembl; ENSMUST00000031341; ENSMUSP00000031341; ENSMUSG00000029394.
DR Ensembl; ENSMUST00000104959; ENSMUSP00000100565; ENSMUSG00000078154.
DR GeneID; 13445; -.
DR KEGG; mmu:13445; -.
DR UCSC; uc008zpo.1; mouse.
DR CTD; 8099; -.
DR MGI; MGI:1202069; Cdk2ap1.
DR VEuPathDB; HostDB:ENSMUSG00000029394; -.
DR VEuPathDB; HostDB:ENSMUSG00000078154; -.
DR eggNOG; KOG4713; Eukaryota.
DR GeneTree; ENSGT00940000155149; -.
DR HOGENOM; CLU_130479_1_0_1; -.
DR InParanoid; O35207; -.
DR OMA; MNAEYFG; -.
DR OrthoDB; 1630301at2759; -.
DR PhylomeDB; O35207; -.
DR TreeFam; TF101037; -.
DR BioGRID-ORCS; 13445; 6 hits in 72 CRISPR screens.
DR ChiTaRS; Cdk2ap1; mouse.
DR PRO; PR:O35207; -.
DR Proteomes; UP000000589; Chromosome 11.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; O35207; protein.
DR Bgee; ENSMUSG00000029394; Expressed in undifferentiated genital tubercle and 59 other tissues.
DR ExpressionAtlas; O35207; baseline and differential.
DR Genevisible; O35207; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:0070182; F:DNA polymerase binding; ISO:MGI.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0060325; P:face morphogenesis; IMP:MGI.
DR GO; GO:0001701; P:in utero embryonic development; IMP:MGI.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; ISO:MGI.
DR InterPro; IPR017266; DOC_1/2.
DR PANTHER; PTHR22607; PTHR22607; 1.
DR Pfam; PF09806; CDK2AP; 1.
DR PIRSF; PIRSF037709; CDK2-associated_p2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Disulfide bond; Phosphoprotein; Reference proteome;
KW Tumor suppressor.
FT CHAIN 1..114
FT /note="Cyclin-dependent kinase 2-associated protein 1"
FT /id="PRO_0000089454"
FT REGION 18..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 19..24
FT /note="Interaction with CDK2AP2"
FT /evidence="ECO:0000250|UniProtKB:O14519"
FT COMPBIAS 19..57
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 45
FT /note="Phosphoserine; by IKKE"
FT /evidence="ECO:0000250|UniProtKB:O14519"
FT DISULFID 104
FT /note="Interchain"
FT /evidence="ECO:0000255"
SQ SEQUENCE 114 AA; 12354 MW; 6421DD87E438F02D CRC64;
MSYKPNLTAH MPAAALNAGS VHSPSTSMAT SSQYRQLLSD YGPPSLGYTQ GTGNSQVPQS
KYAELLAIIE ELGKEIRPTY AGSKSAMERL KRGIIHARSL VRECLAETER NARS
