CDKA2_HUMAN
ID CDKA2_HUMAN Reviewed; 126 AA.
AC O75956;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Cyclin-dependent kinase 2-associated protein 2;
DE Short=CDK2-associated protein 2;
DE AltName: Full=DOC-1-related protein;
DE Short=DOC-1R;
GN Name=CDK2AP2; Synonyms=DOC1R;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10082655; DOI=10.1006/bbrc.1999.0148;
RA Zhang X., Tsao H., Tsuji T., Minoshima S., McBride J., Majewski P.,
RA Todd R., Shimizu N., Wong D.T., Housman D.E., Haluska F.G.;
RT "Identification and mutation analysis of DOC-1R, a DOC-1 growth suppressor-
RT related gene.";
RL Biochem. Biophys. Res. Commun. 255:59-63(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH CDK2AP1.
RX PubMed=14985111; DOI=10.1016/j.bbrc.2004.02.003;
RA Buajeeb W., Zhang X., Ohyama H., Han D., Surarit R., Kim Y., Wong D.T.;
RT "Interaction of the CDK2-associated protein-1, p12(DOC-1/CDK2AP1), with its
RT homolog, p14(DOC-1R).";
RL Biochem. Biophys. Res. Commun. 315:998-1003(2004).
RN [5]
RP FUNCTION, AND INTERACTION WITH CDK2.
RX PubMed=23781148; DOI=10.7150/ijbs.5763;
RA Liu Q., Liu X., Gao J., Shi X., Hu X., Wang S., Luo Y.;
RT "Overexpression of DOC-1R inhibits cell cycle G1/S transition by repressing
RT CDK2 expression and activation.";
RL Int. J. Biol. Sci. 9:541-549(2013).
CC -!- FUNCTION: Plays a role in regulating the self-renewal of embryonic stem
CC cells (ESCs) and in maintaining cell survival during terminal
CC differentiation of ESCs. Regulates microtubule organization of
CC metaphase II oocytes (By similarity). Inhibits cell cycle G1/S phase
CC transition by repressing CDK2 expression and activation; represses CDK2
CC activation by inhibiting its interaction with cyclin E and A
CC (PubMed:23781148). {ECO:0000250|UniProtKB:Q9CPY4,
CC ECO:0000269|PubMed:23781148}.
CC -!- SUBUNIT: Interacts with CDK2AP1 (PubMed:14985111). Interacts with CDK2
CC (PubMed:23781148). Interacts with MAPK1 (By similarity).
CC {ECO:0000250|UniProtKB:Q9CPY4, ECO:0000269|PubMed:14985111,
CC ECO:0000269|PubMed:23781148}.
CC -!- INTERACTION:
CC O75956; Q96HT8: MRFAP1L1; NbExp=3; IntAct=EBI-2808135, EBI-748896;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9CPY4}. Nucleus
CC {ECO:0000250|UniProtKB:Q9CPY4}. Note=Accumulates in immature oocytes in
CC the nucleus. During the first meiotic division, accumulates in the
CC cytoplasm and localizes in dots in the vicinity of the chromosomes in a
CC region enriched in microtubules. {ECO:0000250|UniProtKB:Q9CPY4}.
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- PTM: Phosphorylated by MAPK1 and CDK2. {ECO:0000250|UniProtKB:Q9CPY4}.
CC -!- SIMILARITY: Belongs to the CDK2AP family. {ECO:0000305}.
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DR EMBL; AF089814; AAC61745.1; -; mRNA.
DR EMBL; BT006909; AAP35555.1; -; mRNA.
DR EMBL; BC002850; AAH02850.1; -; mRNA.
DR EMBL; BC016704; AAH16704.1; -; mRNA.
DR CCDS; CCDS8169.1; -.
DR RefSeq; NP_005842.1; NM_005851.4.
DR PDB; 2M1L; NMR; -; A/B=61-126.
DR PDBsum; 2M1L; -.
DR AlphaFoldDB; O75956; -.
DR BMRB; O75956; -.
DR SMR; O75956; -.
DR BioGRID; 115555; 18.
DR IntAct; O75956; 18.
DR MINT; O75956; -.
DR STRING; 9606.ENSP00000301488; -.
DR BindingDB; O75956; -.
DR ChEMBL; CHEMBL6010; -.
DR iPTMnet; O75956; -.
DR PhosphoSitePlus; O75956; -.
DR BioMuta; CDK2AP2; -.
DR EPD; O75956; -.
DR jPOST; O75956; -.
DR MassIVE; O75956; -.
DR PaxDb; O75956; -.
DR PeptideAtlas; O75956; -.
DR PRIDE; O75956; -.
DR ProteomicsDB; 50322; -.
DR Antibodypedia; 30451; 174 antibodies from 23 providers.
DR DNASU; 10263; -.
DR Ensembl; ENST00000301488.8; ENSP00000301488.4; ENSG00000167797.8.
DR GeneID; 10263; -.
DR KEGG; hsa:10263; -.
DR MANE-Select; ENST00000301488.8; ENSP00000301488.4; NM_005851.5; NP_005842.1.
DR CTD; 10263; -.
DR DisGeNET; 10263; -.
DR GeneCards; CDK2AP2; -.
DR HGNC; HGNC:30833; CDK2AP2.
DR HPA; ENSG00000167797; Low tissue specificity.
DR neXtProt; NX_O75956; -.
DR OpenTargets; ENSG00000167797; -.
DR PharmGKB; PA128394572; -.
DR VEuPathDB; HostDB:ENSG00000167797; -.
DR eggNOG; KOG4713; Eukaryota.
DR GeneTree; ENSGT00940000160334; -.
DR HOGENOM; CLU_130479_0_0_1; -.
DR InParanoid; O75956; -.
DR OMA; GYVQTMK; -.
DR OrthoDB; 1630301at2759; -.
DR PhylomeDB; O75956; -.
DR TreeFam; TF101037; -.
DR PathwayCommons; O75956; -.
DR SignaLink; O75956; -.
DR BioGRID-ORCS; 10263; 24 hits in 1080 CRISPR screens.
DR ChiTaRS; CDK2AP2; human.
DR GenomeRNAi; 10263; -.
DR Pharos; O75956; Tbio.
DR PRO; PR:O75956; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; O75956; protein.
DR Bgee; ENSG00000167797; Expressed in mucosa of transverse colon and 189 other tissues.
DR ExpressionAtlas; O75956; baseline and differential.
DR Genevisible; O75956; HS.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005874; C:microtubule; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; IMP:UniProtKB.
DR GO; GO:0070507; P:regulation of microtubule cytoskeleton organization; ISS:UniProtKB.
DR GO; GO:2000035; P:regulation of stem cell division; ISS:UniProtKB.
DR InterPro; IPR017266; DOC_1/2.
DR PANTHER; PTHR22607; PTHR22607; 1.
DR Pfam; PF09806; CDK2AP; 1.
DR PIRSF; PIRSF037709; CDK2-associated_p2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..126
FT /note="Cyclin-dependent kinase 2-associated protein 2"
FT /id="PRO_0000079963"
FT REGION 1..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 64..106
FT /note="Interaction with CDK2"
FT /evidence="ECO:0000269|PubMed:23781148"
FT COMPBIAS 12..28
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 67..70
FT /evidence="ECO:0007829|PDB:2M1L"
FT HELIX 72..85
FT /evidence="ECO:0007829|PDB:2M1L"
FT HELIX 88..92
FT /evidence="ECO:0007829|PDB:2M1L"
FT HELIX 96..120
FT /evidence="ECO:0007829|PDB:2M1L"
SQ SEQUENCE 126 AA; 13101 MW; D668D3FA8CE70CA5 CRC64;
MSYKPIAPAP SSTPGSSTPG PGTPVPTGSV PSPSGSVPGA GAPFRPLFND FGPPSMGYVQ
AMKPPGAQGS QSTYTDLLSV IEEMGKEIRP TYAGSKSAME RLKRGIIHAR ALVRECLAET
ERNART
