CDKA2_MOUSE
ID CDKA2_MOUSE Reviewed; 127 AA.
AC Q9CPY4;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Cyclin-dependent kinase 2-associated protein 2;
DE Short=CDK2-associated protein 2;
DE AltName: Full=DOC-1-related protein;
DE Short=DOC-1R;
GN Name=Cdk2ap2; Synonyms=D19Ertd144e, Doc1r;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, INTERACTION WITH MAPK1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP AND PHOSPHORYLATION.
RX PubMed=12944431; DOI=10.1242/dev.00731;
RA Terret M.E., Lefebvre C., Djiane A., Rassinier P., Moreau J., Maro B.,
RA Verlhac M.H.;
RT "DOC1R: a MAP kinase substrate that control microtubule organization of
RT metaphase II mouse oocytes.";
RL Development 130:5169-5177(2003).
RN [4]
RP FUNCTION.
RX PubMed=22548356; DOI=10.1089/scd.2012.0007;
RA Deshpande A.M., Khalid O., Kim J.J., Kim Y., Lindgren A., Clark A.T.,
RA Wong D.T.;
RT "Cdk2ap2 is a novel regulator for self-renewal of murine embryonic stem
RT cells.";
RL Stem Cells Dev. 21:3010-3018(2012).
CC -!- FUNCTION: Plays a role in regulating the self-renewal of embryonic stem
CC cells (ESCs) and in maintaining cell survival during terminal
CC differentiation of ESCs (PubMed:22548356). Regulates microtubule
CC organization of metaphase II oocytes (PubMed:12944431). Inhibits cell
CC cycle G1/S phase transition by repressing CDK2 expression and
CC activation; represses CDK2 activation by inhibiting its interaction
CC with cyclin E and A (By similarity). {ECO:0000250|UniProtKB:O75956,
CC ECO:0000269|PubMed:12944431, ECO:0000269|PubMed:22548356}.
CC -!- SUBUNIT: Interacts with MAPK1 (PubMed:12944431). Interacts with CDK2
CC and CDK2AP1 (By similarity). {ECO:0000250|UniProtKB:O75956,
CC ECO:0000269|PubMed:12944431}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12944431}. Nucleus
CC {ECO:0000269|PubMed:12944431}. Note=Accumulates in immature oocytes in
CC the nucleus. During the first meiotic division, accumulates in the
CC cytoplasm and localizes in dots in the vicinity of the chromosomes in a
CC region enriched in microtubules. {ECO:0000269|PubMed:12944431}.
CC -!- TISSUE SPECIFICITY: Oocytes (at protein level).
CC {ECO:0000269|PubMed:12944431}.
CC -!- PTM: Phosphorylated by MAPK1 and CDK2. {ECO:0000269|PubMed:12944431}.
CC -!- SIMILARITY: Belongs to the CDK2AP family. {ECO:0000305}.
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DR EMBL; AK003686; BAB22939.1; -; mRNA.
DR EMBL; AK012121; BAB28046.1; -; mRNA.
DR EMBL; AK018035; BAB31046.1; -; mRNA.
DR EMBL; BC025656; AAH25656.1; -; mRNA.
DR CCDS; CCDS29414.1; -.
DR RefSeq; NP_080649.1; NM_026373.4.
DR AlphaFoldDB; Q9CPY4; -.
DR BMRB; Q9CPY4; -.
DR SMR; Q9CPY4; -.
DR STRING; 10090.ENSMUSP00000025779; -.
DR iPTMnet; Q9CPY4; -.
DR PhosphoSitePlus; Q9CPY4; -.
DR MaxQB; Q9CPY4; -.
DR PaxDb; Q9CPY4; -.
DR PeptideAtlas; Q9CPY4; -.
DR PRIDE; Q9CPY4; -.
DR Antibodypedia; 30451; 174 antibodies from 23 providers.
DR Ensembl; ENSMUST00000025779; ENSMUSP00000025779; ENSMUSG00000024856.
DR GeneID; 52004; -.
DR KEGG; mmu:52004; -.
DR UCSC; uc008fym.1; mouse.
DR CTD; 10263; -.
DR MGI; MGI:1098779; Cdk2ap2.
DR VEuPathDB; HostDB:ENSMUSG00000024856; -.
DR eggNOG; KOG4713; Eukaryota.
DR GeneTree; ENSGT00940000160334; -.
DR HOGENOM; CLU_130479_0_0_1; -.
DR InParanoid; Q9CPY4; -.
DR OMA; GYVQTMK; -.
DR OrthoDB; 1630301at2759; -.
DR PhylomeDB; Q9CPY4; -.
DR TreeFam; TF101037; -.
DR BioGRID-ORCS; 52004; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Cdk2ap2; mouse.
DR PRO; PR:Q9CPY4; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q9CPY4; protein.
DR Bgee; ENSMUSG00000024856; Expressed in granulocyte and 65 other tissues.
DR ExpressionAtlas; Q9CPY4; baseline and differential.
DR Genevisible; Q9CPY4; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005874; C:microtubule; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; ISS:UniProtKB.
DR GO; GO:0070507; P:regulation of microtubule cytoskeleton organization; IMP:UniProtKB.
DR GO; GO:2000035; P:regulation of stem cell division; IMP:UniProtKB.
DR InterPro; IPR017266; DOC_1/2.
DR PANTHER; PTHR22607; PTHR22607; 1.
DR Pfam; PF09806; CDK2AP; 1.
DR PIRSF; PIRSF037709; CDK2-associated_p2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..127
FT /note="Cyclin-dependent kinase 2-associated protein 2"
FT /id="PRO_0000079964"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 65..107
FT /note="Interaction with CDK2"
FT /evidence="ECO:0000250|UniProtKB:O75956"
FT COMPBIAS 12..28
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 127 AA; 13202 MW; E8299A37B030EEFD CRC64;
MSYKPIAPAP SSTPGSSTPG PGTPVPTAGS VPSPSGSVPG AAAPFRPLFN DFGPPSMGYV
QAMKPPGSQG SQSTYTDLLS VIEEMGKEIR PTYAGSKSAM ERLKRGIIHA RALVRECLAE
TERNART
