ID   CDKL1_RAT               Reviewed;         352 AA.
AC   Q66HE7;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Cyclin-dependent kinase-like 1 {ECO:0000305};
DE            EC=2.7.11.22;
GN   Name=Cdkl1 {ECO:0000312|RGD:1305080};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.22;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.22;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- DOMAIN: The [NKR]KIAxRE motif seems to be a cyclin-binding region.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr
CC       protein kinase family. CDC2/CDKX subfamily. {ECO:0000305}.
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DR   EMBL; BC081896; AAH81896.1; -; mRNA.
DR   RefSeq; NP_001020292.1; NM_001025121.1.
DR   RefSeq; XP_006240233.1; XM_006240171.3.
DR   RefSeq; XP_006240234.1; XM_006240172.3.
DR   AlphaFoldDB; Q66HE7; -.
DR   SMR; Q66HE7; -.
DR   STRING; 10116.ENSRNOP00000006374; -.
DR   PaxDb; Q66HE7; -.
DR   PRIDE; Q66HE7; -.
DR   GeneID; 314198; -.
DR   KEGG; rno:314198; -.
DR   UCSC; RGD:1305080; rat.
DR   CTD; 8814; -.
DR   RGD; 1305080; Cdkl1.
DR   VEuPathDB; HostDB:ENSRNOG00000038720; -.
DR   eggNOG; KOG0593; Eukaryota.
DR   HOGENOM; CLU_000288_181_1_1; -.
DR   InParanoid; Q66HE7; -.
DR   OMA; MRFPTVA; -.
DR   OrthoDB; 398098at2759; -.
DR   PhylomeDB; Q66HE7; -.
DR   TreeFam; TF101031; -.
DR   PRO; PR:Q66HE7; -.
DR   Proteomes; UP000002494; Chromosome 6.
DR   Bgee; ENSRNOG00000038720; Expressed in adult mammalian kidney and 18 other tissues.
DR   GO; GO:0035869; C:ciliary transition zone; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004693; F:cyclin-dependent protein serine/threonine kinase activity; IBA:GO_Central.
DR   GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR   GO; GO:0007507; P:heart development; IEP:RGD.
DR   GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR   GO; GO:1902017; P:regulation of cilium assembly; ISS:UniProtKB.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR017441; Protein_kinase_ATP_BS.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Kinase; Nucleotide-binding; Nucleus;
KW   Reference proteome; Serine/threonine-protein kinase; Transferase.
FT   CHAIN           1..352
FT                   /note="Cyclin-dependent kinase-like 1"
FT                   /id="PRO_0000085812"
FT   DOMAIN          4..287
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   MOTIF           45..51
FT                   /note="[NKR]KIAxRE"
FT   ACT_SITE        126
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT                   ECO:0000255|PROSITE-ProRule:PRU10027"
FT   BINDING         10..18
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         33
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ   SEQUENCE   352 AA;  40899 MW;  DB9CDF5464E66E24 CRC64;
     MEKYEKIGKI GEGSYGVVFK CRNRDTGQIV AIKRFLETED DPVIKKIALR EIRMLKQLKH
     PNLVSLLEVF RRKRRLHLVF EYCHHTVLHE LDRYQRGVPE PLVKNITWQT LQAVNFCHKH
     NCIHRDVKPE NILITKHSVI KLCDFGFARL LTGPGDYYTD YVATRWYRSP ELLVGDTQYG
     PPVDVWAIGC VFAELLSGVP LWPGKSDVDQ LYLIRKTLGD LIPRHQQVFS MNQYFSGVKI
     PDPEDMETLE LKFPNISYSA LGFLKGCLHM DPAERLTCEQ LLQHPYFDSI RDVGELARPH
     DKPTRKTLRQ SRKHLTGLQH LPQLTSSSVL PALDRKKYHC GTRNFNYHFP NI