CDKN3_RAT
ID CDKN3_RAT Reviewed; 212 AA.
AC B2RZ50;
DT 10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Cyclin-dependent kinase inhibitor 3 {ECO:0000250|UniProtKB:Q16667};
DE EC=3.1.3.16;
DE EC=3.1.3.48;
DE AltName: Full=CDK2-associated dual-specificity phosphatase {ECO:0000250|UniProtKB:Q16667};
DE AltName: Full=Kinase-associated phosphatase {ECO:0000250|UniProtKB:Q16667};
GN Name=Cdkn3 {ECO:0000312|EMBL:AAI67026.1, ECO:0000312|RGD:1307781};
GN Synonyms=Kap {ECO:0000250|UniProtKB:Q16667};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1] {ECO:0000312|EMBL:EDL88327.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway {ECO:0000312|EMBL:EDL88327.1};
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000312|EMBL:AAI67026.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Brown Norway/NHsdMcwi {ECO:0000269|PubMed:15489334};
RC TISSUE=Embryonic liver {ECO:0000312|EMBL:AAI67026.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May play a role in cell cycle regulation. Dual specificity
CC phosphatase active toward substrates containing either phosphotyrosine
CC or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a
CC cyclin-dependent manner (By similarity).
CC {ECO:0000250|UniProtKB:Q16667}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:10684, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620; EC=3.1.3.48;
CC Evidence={ECO:0000250|UniProtKB:Q16667};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83421; EC=3.1.3.16;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC -!- SUBUNIT: Interacts with cyclin-dependent kinases such as CDK1, CDK2 and
CC CDK3. Does not interact with CDK4. Interacts (via C-terminus) with
CC phosphorylated CDK2 (via C-terminal helix). Interacts with MS4A3 (via
CC C-terminus); the interaction enhances CDKN3 enzymatic activity (By
CC similarity). {ECO:0000250|UniProtKB:Q16667}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC {ECO:0000250|UniProtKB:Q16667}.
CC -!- SIMILARITY: Belongs to the protein-tyrosine phosphatase family.
CC {ECO:0000255}.
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DR EMBL; CH474040; EDL88327.1; -; Genomic_DNA.
DR EMBL; BC167026; AAI67026.1; -; mRNA.
DR RefSeq; XP_006251853.1; XM_006251791.3.
DR AlphaFoldDB; B2RZ50; -.
DR SMR; B2RZ50; -.
DR STRING; 10116.ENSRNOP00000012985; -.
DR PhosphoSitePlus; B2RZ50; -.
DR PaxDb; B2RZ50; -.
DR Ensembl; ENSRNOT00000012985; ENSRNOP00000012985; ENSRNOG00000009785.
DR GeneID; 289993; -.
DR CTD; 1033; -.
DR RGD; 1307781; Cdkn3.
DR eggNOG; KOG1720; Eukaryota.
DR GeneTree; ENSGT00390000004717; -.
DR HOGENOM; CLU_047330_1_0_1; -.
DR InParanoid; B2RZ50; -.
DR OMA; PFHISWL; -.
DR OrthoDB; 1539111at2759; -.
DR PhylomeDB; B2RZ50; -.
DR TreeFam; TF101040; -.
DR PRO; PR:B2RZ50; -.
DR Proteomes; UP000002494; Chromosome 15.
DR Proteomes; UP000234681; Chromosome 15.
DR Bgee; ENSRNOG00000009785; Expressed in testis and 18 other tissues.
DR Genevisible; B2RZ50; RN.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0004722; F:protein serine/threonine phosphatase activity; ISO:RGD.
DR GO; GO:0004725; F:protein tyrosine phosphatase activity; ISO:RGD.
DR GO; GO:0008138; F:protein tyrosine/serine/threonine phosphatase activity; IEA:InterPro.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0006470; P:protein dephosphorylation; IEA:InterPro.
DR GO; GO:0051726; P:regulation of cell cycle; ISO:RGD.
DR Gene3D; 3.90.190.10; -; 1.
DR InterPro; IPR008425; CDK_inhib_3.
DR InterPro; IPR022778; CDKN3.
DR InterPro; IPR029021; Prot-tyrosine_phosphatase-like.
DR InterPro; IPR003595; Tyr_Pase_cat.
DR InterPro; IPR000387; Tyr_Pase_dom.
DR InterPro; IPR020422; TYR_PHOSPHATASE_DUAL_dom.
DR Pfam; PF05706; CDKN3; 1.
DR PIRSF; PIRSF037322; CDKN3; 1.
DR SMART; SM00404; PTPc_motif; 1.
DR SUPFAM; SSF52799; SSF52799; 1.
DR PROSITE; PS50056; TYR_PHOSPHATASE_2; 1.
DR PROSITE; PS50054; TYR_PHOSPHATASE_DUAL; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cytoplasm; Hydrolase; Protein phosphatase; Reference proteome.
FT CHAIN 1..212
FT /note="Cyclin-dependent kinase inhibitor 3"
FT /id="PRO_0000396639"
FT DOMAIN 32..201
FT /note="Tyrosine-protein phosphatase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00160"
FT REGION 1..34
FT /note="Interaction with CDK2"
FT /evidence="ECO:0000250|UniProtKB:Q16667"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 140
FT /note="Phosphocysteine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00160"
SQ SEQUENCE 212 AA; 23774 MW; 345F42838AA50801 CRC64;
MKPPISIQAS EFDSSDEEPA DDEQTPIQIS WLPLSRVNCS QFLGLCALPG CKFKDVRRNI
QKDTEELKSS GIQDVFVFCT RGELSKYRVP NLLDLYQQYG IVTHHHPIPD GGTPDIGSCW
EIMEELATCL KNNRKTLIHC YGGLGRSCLV AACLLLYLSD SISPQQAIDS LRDVRGSGAI
QTIKQYNYLH EFRDKLAAYL SSRDSLSRSV SR
