CDN2B_MOUSE
ID CDN2B_MOUSE Reviewed; 130 AA.
AC P55271;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Cyclin-dependent kinase 4 inhibitor B;
DE AltName: Full=p14-INK4b;
DE AltName: Full=p15-INK4b;
GN Name=Cdkn2b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=7651726;
RA Quelle D.E., Ashmun R.A., Hannon G.J., Rehberger P.A., Trono D.,
RA Richter K.H., Walker C., Beach D., Sherr C.J., Serrano M.;
RT "Cloning and characterization of murine p16INK4a and p15INK4b genes.";
RL Oncogene 11:635-645(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C57BL/6J X DBA;
RX PubMed=9178896; DOI=10.1038/sj.onc.1200969;
RA Malumbres M., de Castro I.P., Santos J., Melendez B., Mangues R.,
RA Serrano M., Pellicer A., Fernandez-Piqueras J.;
RT "Inactivation of the cyclin-dependent kinase inhibitor p15INK4b by deletion
RT and de novo methylation with independence of p16INK4a alterations in murine
RT primary T-cell lymphomas.";
RL Oncogene 14:1361-1370(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-12, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic brain;
RX PubMed=15345747; DOI=10.1074/mcp.m400085-mcp200;
RA Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
RT "Phosphoproteomic analysis of the developing mouse brain.";
RL Mol. Cell. Proteomics 3:1093-1101(2004).
RN [5]
RP STRUCTURE BY NMR.
RX PubMed=10556039; DOI=10.1006/jmbi.1999.3231;
RA Yuan C., Li J., Selby T.L., Byeon I.-J., Tsai M.-D.;
RT "Tumor suppressor INK4: comparisons of conformational properties between
RT p16(INK4A) and p18(INK4C).";
RL J. Mol. Biol. 294:201-211(1999).
CC -!- FUNCTION: Interacts strongly with CDK4 and CDK6. Potent inhibitor.
CC Potential effector of TGF-beta induced cell cycle arrest (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer of CDKN2B with CDK4 or CDK6. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed ubiquitously.
CC -!- INDUCTION: By TGF-beta.
CC -!- SIMILARITY: Belongs to the CDKN2 cyclin-dependent kinase inhibitor
CC family. {ECO:0000305}.
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DR EMBL; U66085; AAB39833.1; -; Genomic_DNA.
DR EMBL; U66084; AAB39833.1; JOINED; Genomic_DNA.
DR EMBL; BC002010; AAH02010.1; -; mRNA.
DR CCDS; CCDS18351.1; -.
DR RefSeq; NP_031696.1; NM_007670.4.
DR PDB; 1D9S; NMR; -; A=1-130.
DR PDBsum; 1D9S; -.
DR AlphaFoldDB; P55271; -.
DR BMRB; P55271; -.
DR SMR; P55271; -.
DR BioGRID; 198655; 1.
DR DIP; DIP-60249N; -.
DR IntAct; P55271; 1.
DR STRING; 10090.ENSMUSP00000095595; -.
DR iPTMnet; P55271; -.
DR PhosphoSitePlus; P55271; -.
DR EPD; P55271; -.
DR PaxDb; P55271; -.
DR PeptideAtlas; P55271; -.
DR PRIDE; P55271; -.
DR ProteomicsDB; 281521; -.
DR Antibodypedia; 3605; 635 antibodies from 38 providers.
DR DNASU; 12579; -.
DR Ensembl; ENSMUST00000097981; ENSMUSP00000095595; ENSMUSG00000073802.
DR GeneID; 12579; -.
DR KEGG; mmu:12579; -.
DR UCSC; uc008tok.2; mouse.
DR CTD; 1030; -.
DR MGI; MGI:104737; Cdkn2b.
DR VEuPathDB; HostDB:ENSMUSG00000073802; -.
DR eggNOG; KOG0504; Eukaryota.
DR GeneTree; ENSGT00940000162423; -.
DR HOGENOM; CLU_000134_37_1_1; -.
DR InParanoid; P55271; -.
DR OMA; RDEDKGM; -.
DR OrthoDB; 1435166at2759; -.
DR PhylomeDB; P55271; -.
DR TreeFam; TF352389; -.
DR Reactome; R-MMU-2559580; Oxidative Stress Induced Senescence.
DR Reactome; R-MMU-2559582; Senescence-Associated Secretory Phenotype (SASP).
DR Reactome; R-MMU-2559585; Oncogene Induced Senescence.
DR Reactome; R-MMU-69231; Cyclin D associated events in G1.
DR BioGRID-ORCS; 12579; 0 hits in 75 CRISPR screens.
DR EvolutionaryTrace; P55271; -.
DR PRO; PR:P55271; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; P55271; protein.
DR Bgee; ENSMUSG00000073802; Expressed in small intestine Peyer's patch and 151 other tissues.
DR ExpressionAtlas; P55271; baseline and differential.
DR Genevisible; P55271; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; ISO:MGI.
DR GO; GO:0019901; F:protein kinase binding; ISO:MGI.
DR GO; GO:0007568; P:aging; IEA:Ensembl.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0031668; P:cellular response to extracellular stimulus; ISO:MGI.
DR GO; GO:0031670; P:cellular response to nutrient; ISO:MGI.
DR GO; GO:0071560; P:cellular response to transforming growth factor beta stimulus; IEA:Ensembl.
DR GO; GO:0090398; P:cellular senescence; ISO:MGI.
DR GO; GO:0001889; P:liver development; IEA:Ensembl.
DR GO; GO:0030219; P:megakaryocyte differentiation; IEA:Ensembl.
DR GO; GO:1902807; P:negative regulation of cell cycle G1/S phase transition; ISO:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0050680; P:negative regulation of epithelial cell proliferation; ISO:MGI.
DR GO; GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; IMP:MGI.
DR GO; GO:0060253; P:negative regulation of glial cell proliferation; ISO:MGI.
DR GO; GO:0042326; P:negative regulation of phosphorylation; ISO:MGI.
DR GO; GO:0030858; P:positive regulation of epithelial cell differentiation; IEA:Ensembl.
DR GO; GO:0030511; P:positive regulation of transforming growth factor beta receptor signaling pathway; ISO:MGI.
DR GO; GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; ISO:MGI.
DR GO; GO:0070316; P:regulation of G0 to G1 transition; ISO:MGI.
DR GO; GO:2000045; P:regulation of G1/S transition of mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0034097; P:response to cytokine; IEA:Ensembl.
DR GO; GO:0014070; P:response to organic cyclic compound; IEA:Ensembl.
DR GO; GO:0048536; P:spleen development; IGI:MGI.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR Pfam; PF12796; Ank_2; 1.
DR SMART; SM00248; ANK; 3.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ANK repeat; Cell cycle; Phosphoprotein; Reference proteome;
KW Repeat; Tumor suppressor.
FT CHAIN 1..130
FT /note="Cyclin-dependent kinase 4 inhibitor B"
FT /id="PRO_0000144185"
FT REPEAT 5..34
FT /note="ANK 1"
FT REPEAT 38..66
FT /note="ANK 2"
FT REPEAT 71..100
FT /note="ANK 3"
FT REPEAT 104..130
FT /note="ANK 4"
FT MOD_RES 12
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:15345747"
FT HELIX 9..15
FT /evidence="ECO:0007829|PDB:1D9S"
FT HELIX 19..27
FT /evidence="ECO:0007829|PDB:1D9S"
FT TURN 41..45
FT /evidence="ECO:0007829|PDB:1D9S"
FT HELIX 51..60
FT /evidence="ECO:0007829|PDB:1D9S"
FT TURN 69..72
FT /evidence="ECO:0007829|PDB:1D9S"
FT HELIX 75..82
FT /evidence="ECO:0007829|PDB:1D9S"
FT HELIX 85..93
FT /evidence="ECO:0007829|PDB:1D9S"
FT STRAND 102..106
FT /evidence="ECO:0007829|PDB:1D9S"
FT HELIX 108..115
FT /evidence="ECO:0007829|PDB:1D9S"
FT HELIX 118..128
FT /evidence="ECO:0007829|PDB:1D9S"
SQ SEQUENCE 130 AA; 13789 MW; 7AAD60FF552BCFF9 CRC64;
MLGGSSDAGL ATAAARGQVE TVRQLLEAGA DPNALNRFGR RPIQVMMMGS AQVAELLLLH
GAEPNCADPA TLTRPVHDAA REGFLDTLVV LHRAGARLDV CDAWGRLPVD LAEEQGHRDI
ARYLHAATGD
