ID   CDND_BACCX              Reviewed;         331 AA.
AC   R8NBN0;
DT   10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2013, sequence version 1.
DT   25-MAY-2022, entry version 20.
DE   RecName: Full=Probable cyclic nucleotide synthase IK1_05630 {ECO:0000305};
DE            EC=2.7.7.- {ECO:0000305};
GN   Name=cdnD {ECO:0000303|PubMed:30787435};
GN   ORFNames=IK1_05630 {ECO:0000312|EMBL:EOP43920.1};
OS   Bacillus cereus (strain VD146).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1053236;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VD146;
RG   The Broad Institute Genome Sequencing Platform;
RG   The Broad Institute Genome Sequencing Center for Infectious Disease;
RA   Feldgarden M., Van der Auwera G.A., Mahillon J., Duprez V., Timmery S.,
RA   Mattelet C., Dierick K., Sun M., Yu Z., Zhu L., Hu X., Shank E.B.,
RA   Swiecicka I., Hansen B.M., Andrup L., Walker B., Young S.K., Zeng Q.,
RA   Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA   Arachchi H.M., Berlin A.M., Chapman S.B., Dewar J., Goldberg J., Griggs A.,
RA   Gujja S., Hansen M., Howarth C., Imamovic A., Larimer J., McCowan C.,
RA   Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA   Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT   "The genome sequence of Bacillus cereus VD146.";
RL   Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NOMENCLATURE, AND SIMILARITY.
RC   STRAIN=VD146;
RX   PubMed=30787435; DOI=10.1038/s41586-019-0953-5;
RA   Whiteley A.T., Eaglesham J.B., de Oliveira Mann C.C., Morehouse B.R.,
RA   Lowey B., Nieminen E.A., Danilchanka O., King D.S., Lee A.S.Y.,
RA   Mekalanos J.J., Kranzusch P.J.;
RT   "Bacterial cGAS-like enzymes synthesize diverse nucleotide signals.";
RL   Nature 567:194-199(2019).
RN   [3]
RP   ANTIVIRAL DEFENSE, NOMENCLATURE, AND OPERON STRUCTURE.
RC   STRAIN=VD146;
RX   PubMed=31533127; DOI=10.1038/s41586-019-1605-5;
RA   Cohen D., Melamed S., Millman A., Shulman G., Oppenheimer-Shaanan Y.,
RA   Kacen A., Doron S., Amitai G., Sorek R.;
RT   "Cyclic GMP-AMP signalling protects bacteria against viral infection.";
RL   Nature 574:691-695(2019).
RN   [4]
RP   CLASSIFICATION AND NOMENCLATURE.
RX   PubMed=32839535; DOI=10.1038/s41564-020-0777-y;
RA   Millman A., Melamed S., Amitai G., Sorek R.;
RT   "Diversity and classification of cyclic-oligonucleotide-based anti-phage
RT   signalling systems.";
RL   Nat. Microbiol. 5:1608-1615(2020).
CC   -!- FUNCTION: CBASS (cyclic oligonucleotide-based antiphage signaling
CC       system) provides immunity against bacteriophage. The CD-NTase protein
CC       synthesizes cyclic nucleotides in response to infection; these serve as
CC       specific second messenger signals. The signals activate a diverse range
CC       of effectors, leading to bacterial cell death and thus abortive phage
CC       infection (PubMed:31533127). A type I-B CBASS system (PubMed:32839535).
CC       {ECO:0000269|PubMed:31533127, ECO:0000303|PubMed:32839535}.
CC   -!- FUNCTION: Probably a cyclic nucleotide synthase that makes second
CC       messenger nucleotide which activates a CBASS antiviral defense system.
CC       {ECO:0000305|PubMed:30787435, ECO:0000305|PubMed:31533127}.
CC   -!- FUNCTION: Protects B.subtilis against phage infection. When IK1_05630
CC       and IK1_05631 are introduced in B.subtilis BEST7003 there is 1000-fold
CC       protection against phage SBSphiC. Both genes are required for
CC       protection. Activation leads to bacterial cell lysis and death, which
CC       occurs before the phage has finished its replication cycle, thus
CC       protecting non-infected bacteria by aborting the phage infection and
CC       preventing its propagation. {ECO:0000269|PubMed:31533127}.
CC   -!- INDUCTION: Part of the CBASS operon consisting of cdnD-IK1_05631.
CC       {ECO:0000305|PubMed:31533127}.
CC   -!- SIMILARITY: Belongs to the CD-NTase family. D12 subfamily.
CC       {ECO:0000305|PubMed:30787435}.
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DR   EMBL; AHFE01000034; EOP43920.1; -; Genomic_DNA.
DR   RefSeq; WP_016119572.1; NZ_KB976676.1.
DR   AlphaFoldDB; R8NBN0; -.
DR   SMR; R8NBN0; -.
DR   EnsemblBacteria; EOP43920; EOP43920; IK1_05630.
DR   PATRIC; fig|1053236.3.peg.1317; -.
DR   HOGENOM; CLU_051351_0_1_9; -.
DR   Proteomes; UP000014020; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0009117; P:nucleotide metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR043519; NT_sf.
DR   SUPFAM; SSF81301; SSF81301; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; ATP-binding; Nucleotide metabolism; Nucleotide-binding;
KW   Nucleotidyltransferase; Transferase.
FT   CHAIN           1..331
FT                   /note="Probable cyclic nucleotide synthase IK1_05630"
FT                   /id="PRO_0000451854"
SQ   SEQUENCE   331 AA;  38253 MW;  097ADF73AEB170C3 CRC64;
     MVKTANSAYD NFIKNSVDLD SEQTGIARNS RDWLYGNLKN FPKVVENFPK LYAGKEIIGF
     GSFRRRTKIR PLDDIDLMLV FTGEGTTYTE YADKIVLNVP GDADKLRKLV NDDGTLNSIR
     VIEKIKSSLS IIPQYKKAEI HRRQEATTLE LSTYDWVFDI VPAFITSENA YGKSYYIIPD
     GSGNWKKTDP RIDNERATET NQKFDGNVLG LIRVIKYWNT NRSKKISSYL MENIVLDYFQ
     NKFYWNGMKQ ELRGFFEYLK TSIYNSHYDP KGIQGDLNTL DYNTKLSISN QASGCFENID
     LAIRYESLGQ HKEAIECWKK VFGDEFPSHE D