ID   CDS1_CONLT              Reviewed;          85 AA.
AC   F6JWU7;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 1.
DT   25-MAY-2022, entry version 13.
DE   RecName: Full=Alpha-conotoxin Lt28.1 {ECO:0000305|PubMed:28666820};
DE   AltName: Full=Conotoxin Lt15.2 {ECO:0000312|EMBL:ADZ76484.1};
DE   AltName: Full=Conotoxin Lt28.1 {ECO:0000303|PubMed:28666820};
DE   Flags: Precursor;
OS   Conus litteratus (Lettered cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Elisaconus.
OX   NCBI_TaxID=89445;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND RECOMBINANT EXPRESSION.
RC   TISSUE=Venom duct;
RX   PubMed=28666820; DOI=10.1016/j.peptides.2017.06.008;
RA   Lu J., Zhang K., Wang S., Sun T., Yu S., Dai Q., Liu Z.;
RT   "Cloning, expression and functional characterization of a D-superfamily
RT   conotoxin Lt28.1 with previously undescribed cysteine pattern.";
RL   Peptides 94:64-70(2017).
CC   -!- FUNCTION: Alpha-conotoxins act on postsynaptic membranes, they bind to
CC       the nicotinic acetylcholine receptors (nAChR) and thus inhibit them.
CC       This toxin weakly inhibits alpha-9-alpha-10/CHRNA9-CHRNA10 nAChRs
CC       (IC(50)=3 uM). {ECO:0000269|PubMed:28666820}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:28666820}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:28666820}.
CC   -!- DOMAIN: The cysteine framework is XXVIII (C-C-C-CC-C-C-C-C-C).
CC       {ECO:0000305}.
CC   -!- PTM: Contains 5 disulfide bonds. {ECO:0000305}.
CC   -!- MISCELLANEOUS: Very weakly inhibits alpha-3-beta-2/CHRNA3-CHRNB2 (19.4%
CC       when 10 uM of toxin is tested) and alpha-4-beta-2/CHRNA4-CHRNB2 (18% at
CC       10 uM) nAChRs subtypes. Has no effect on alpha-3-beta-4/CHRNA3-CHRNB4,
CC       alpha-4-beta-4/CHRNA4-CHRNB4, alpha-2-beta-2/CHRNA2-CHRNB2, alpha-2-
CC       beta-4/CHRNA2-CHRNB4, alpha-7/CHRNA7 and alpha-6/alpha-3-beta-2-beta-3
CC       (CHRNA6/CHRNA3-CHRNB2-CHRNB3) nAChRs subtypes.
CC       {ECO:0000269|PubMed:28666820}.
CC   -!- SIMILARITY: Belongs to the conotoxin D superfamily. {ECO:0000305}.
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DR   EMBL; HM003926; ADZ76484.1; -; mRNA.
DR   AlphaFoldDB; F6JWU7; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0035792; C:host cell postsynaptic membrane; IEA:UniProtKB-KW.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Acetylcholine receptor inhibiting toxin; Disulfide bond; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..40
FT                   /evidence="ECO:0000305|PubMed:28666820"
FT                   /id="PRO_0000451012"
FT   CHAIN           41..85
FT                   /note="Alpha-conotoxin Lt28.1"
FT                   /evidence="ECO:0000305|PubMed:28666820"
FT                   /id="PRO_5003337452"
SQ   SEQUENCE   85 AA;  9391 MW;  84F7AAB262566FFF CRC64;
     MPKLEMMLLV LLILPLCYID AVGPPPPWNM EDEIIEHWQK LHCHEISDLT PWILCSPEPL
     CGGKGCCAQE VCDCSGPACT CPPCL