CDT1_SCHPO
ID CDT1_SCHPO Reviewed; 444 AA.
AC P40382; Q9URL6;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 14-AUG-2001, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Cell division cycle protein cdt1;
GN Name=cdt1; ORFNames=SPBC428.18;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=8313888; DOI=10.1002/j.1460-2075.1994.tb06277.x;
RA Hofmann J.F.X., Beach D.;
RT "cdt1 is an essential target of the Cdc10/Sct1 transcription factor:
RT requirement for DNA replication and inhibition of mitosis.";
RL EMBO J. 13:425-434(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION, INTERACTION WITH CDC18, AND SUBCELLULAR LOCATION.
RX PubMed=10766248; DOI=10.1038/35007110;
RA Nishitani H., Lygerou Z., Nishimoto T., Nurse P.;
RT "The Cdt1 protein is required to license DNA for replication in fission
RT yeast.";
RL Nature 404:625-628(2000).
RN [4]
RP FUNCTION.
RX PubMed=11532929; DOI=10.1093/emboj/20.17.4648;
RA Yanow S.K., Lygerou Z., Nurse P.;
RT "Expression of Cdc18/Cdc6 and Cdt1 during G2 phase induces initiation of
RT DNA replication.";
RL EMBO J. 20:4648-4656(2001).
RN [5]
RP UBIQUITINATION.
RX PubMed=17039252; DOI=10.1038/sj.embor.7400827;
RA Ralph E., Boye E., Kearsey S.E.;
RT "DNA damage induces Cdt1 proteolysis in fission yeast through a pathway
RT dependent on Cdt2 and Ddb1.";
RL EMBO Rep. 7:1134-1139(2006).
CC -!- FUNCTION: DNA replication licensing factor, required for pre-
CC replication complex assembly. Faithful duplication of the genetic
CC material requires 'once per cell cycle' DNA replication initiation and
CC elongation. Central to this control is the tightly regulated formation
CC of prereplicative complexes (preRCs) at future origins of DNA
CC replication. Required for the recruitment of the MCM helicase complex
CC to the replication origins. {ECO:0000269|PubMed:10766248,
CC ECO:0000269|PubMed:11532929, ECO:0000269|PubMed:8313888}.
CC -!- SUBUNIT: Interacts with cdc18. {ECO:0000269|PubMed:10766248}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10766248}.
CC Note=Present in the nucleus after mitosis when DNA licensing takes
CC place.
CC -!- PTM: Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2)
CC complex, leading to its degradation. {ECO:0000269|PubMed:17039252}.
CC -!- SIMILARITY: Belongs to the Cdt1 family. {ECO:0000305}.
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DR EMBL; Z27248; CAA81761.1; -; mRNA.
DR EMBL; CU329671; CAA22293.1; -; Genomic_DNA.
DR PIR; S41721; S41721.
DR RefSeq; NP_595196.1; NM_001021103.2.
DR AlphaFoldDB; P40382; -.
DR BioGRID; 277364; 9.
DR IntAct; P40382; 1.
DR MINT; P40382; -.
DR STRING; 4896.SPBC428.18.1; -.
DR PaxDb; P40382; -.
DR EnsemblFungi; SPBC428.18.1; SPBC428.18.1:pep; SPBC428.18.
DR GeneID; 2540847; -.
DR KEGG; spo:SPBC428.18; -.
DR PomBase; SPBC428.18; cdt1.
DR VEuPathDB; FungiDB:SPBC428.18; -.
DR eggNOG; KOG4762; Eukaryota.
DR HOGENOM; CLU_654093_0_0_1; -.
DR InParanoid; P40382; -.
DR OMA; FHISINT; -.
DR Reactome; R-SPO-68949; Orc1 removal from chromatin.
DR Reactome; R-SPO-68962; Activation of the pre-replicative complex.
DR PRO; PR:P40382; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0000785; C:chromatin; IDA:PomBase.
DR GO; GO:0005656; C:nuclear pre-replicative complex; ISO:PomBase.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0070182; F:DNA polymerase binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000076; P:DNA replication checkpoint signaling; IBA:GO_Central.
DR GO; GO:0071163; P:DNA replication preinitiation complex assembly; IBA:GO_Central.
DR GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR GO; GO:1902985; P:mitotic pre-replicative complex assembly; ISO:PomBase.
DR GO; GO:0030174; P:regulation of DNA-templated DNA replication initiation; IBA:GO_Central.
DR GO; GO:1903466; P:regulation of mitotic DNA replication initiation; IMP:PomBase.
DR Gene3D; 1.10.10.1420; -; 1.
DR InterPro; IPR045173; Cdt1.
DR InterPro; IPR032054; Cdt1_C.
DR InterPro; IPR038090; Cdt1_C_WH_dom_sf.
DR InterPro; IPR014939; CDT1_Gemini-bd-like.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR28637; PTHR28637; 1.
DR Pfam; PF08839; CDT1; 1.
DR Pfam; PF16679; CDT1_C; 1.
DR SMART; SM01075; CDT1; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Mitosis; Nucleus; Reference proteome;
KW Ubl conjugation.
FT CHAIN 1..444
FT /note="Cell division cycle protein cdt1"
FT /id="PRO_0000089459"
FT CONFLICT 42
FT /note="S -> L (in Ref. 1; CAA81761)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 444 AA; 49956 MW; 653BDD1CDDFE8600 CRC64;
MKCRALYSHD NNILIFGFCV QIMSAGSQTK LNFSVRKTRS SSKRSNAAII EPPKNPEDSQ
IIPAVKRLKE NLDTESLEQN EVLPPVKNES VLFLEKVFNA VDICVKFHLS INTKPTFVLL
ENKVSGLTKI SLKITHLAQI LTVWPESFAI TPCFTIHQGK RVATYELSYP RNANLPEAFS
RSIEFKRRLE KWLLEHCSET EIPAQQLQAL PSLSKNTVNE SSLVRKLNLE KSTSRELRIP
TQTLEPKFTT NTAKYANELV SCSMLDSSST LSKSVNSKIN LKSHQSSSSV QNSSRKLTSS
QLTLRQSSLF DRVRKKQKAM EAKKAEEFKN NLVVHTLAKE KVSFVRIIDL IFVQLSTWPT
KRSFSMSEIV TSMQMSISSS LSPDQCAKAI ELLSKALPAW CTINLLGNIQ VVTFSRIVNG
KPYLRSQLIE ELQTKASITI LSNS
