ID   CDTA_HELHP              Reviewed;         231 AA.
AC   Q9RFY6;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=Cytolethal distending toxin subunit A;
DE            Short=CDT A;
DE   Flags: Precursor;
GN   Name=cdtA; OrderedLocusNames=HH_1446;
OS   Helicobacter hepaticus (strain ATCC 51449 / 3B1).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=235279;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 51449 / 3B1;
RX   PubMed=10603386; DOI=10.1128/iai.68.1.184-191.2000;
RA   Young V.B., Knox K.A., Schauer D.B.;
RT   "Cytolethal distending toxin sequence and activity in the enterohepatic
RT   pathogen Helicobacter hepaticus.";
RL   Infect. Immun. 68:184-191(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51449 / 3B1;
RX   PubMed=12810954; DOI=10.1073/pnas.1332093100;
RA   Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M.,
RA   Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R.,
RA   Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B.,
RA   Shen Z., Weber J., Frosch M., Fox J.G.;
RT   "The complete genome sequence of the carcinogenic bacterium Helicobacter
RT   hepaticus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003).
CC   -!- FUNCTION: CDTs are cytotoxins which induce cell distension, growth
CC       arrest in G2/M phase, nucleus swelling, and chromatin fragmentation in
CC       HeLa cells.
CC   -!- SUBUNIT: Heterotrimer of 3 subunits, CdtA, CdtB and CdtC.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}.
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DR   EMBL; AF163667; AAF19157.1; -; Genomic_DNA.
DR   EMBL; AE017125; AAP78043.1; -; Genomic_DNA.
DR   RefSeq; WP_011116286.1; NC_004917.1.
DR   AlphaFoldDB; Q9RFY6; -.
DR   SMR; Q9RFY6; -.
DR   STRING; 235279.HH_1446; -.
DR   EnsemblBacteria; AAP78043; AAP78043; HH_1446.
DR   KEGG; hhe:HH_1446; -.
DR   eggNOG; ENOG50347HZ; Bacteria.
DR   HOGENOM; CLU_090932_0_0_7; -.
DR   OMA; WLWGYTP; -.
DR   OrthoDB; 935854at2; -.
DR   Proteomes; UP000002495; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00161; RICIN; 1.
DR   InterPro; IPR015957; CDtoxinA.
DR   InterPro; IPR003558; CDtoxinA/C.
DR   InterPro; IPR035992; Ricin_B-like_lectins.
DR   InterPro; IPR000772; Ricin_B_lectin.
DR   Pfam; PF03498; CDtoxinA; 1.
DR   PIRSF; PIRSF036516; CDT_A; 1.
DR   PRINTS; PR01387; CDTOXINA.
DR   SUPFAM; SSF50370; SSF50370; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
DR   PROSITE; PS50231; RICIN_B_LECTIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lectin; Lipoprotein; Membrane; Palmitate;
KW   Reference proteome; Signal; Toxin; Virulence.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           16..231
FT                   /note="Cytolethal distending toxin subunit A"
FT                   /id="PRO_0000013372"
FT   DOMAIN          130..217
FT                   /note="Ricin B-type lectin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00174"
FT   REGION          20..51
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          99..110
FT                   /note="Mediates binding to target cells"
FT                   /evidence="ECO:0000250"
FT   LIPID           16
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           16
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   231 AA;  26021 MW;  55E3BD5FC6E54D86 CRC64;
     MRLLFFLLIT LLFAACSSTP KVHQPKHSSK TEKDLGIGLS PTPPPNERIP GEKSRAMLME
     MEGREIPVRI PDANASKIDK NVSNPISIMS SSGGLLTLWA LKPRNWVWGY TPIDSFEFGR
     AKFWRIISFS NGQVIIKNMQ EGTCLQAYGN GVIHDICDSK NQAQLWNLNF FDNQAIQIQS
     VSAKTCLQTP TVRTTTYYSI YLTKCATSSN LDQQWYITPV ALEPEPIFFI K