CDX1_MOUSE
ID CDX1_MOUSE Reviewed; 268 AA.
AC P18111; Q8VCF7;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Homeobox protein CDX-1;
DE AltName: Full=Caudal-type homeobox protein 1;
GN Name=Cdx1; Synonyms=Cdx-1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ;
RX PubMed=7903305; DOI=10.1016/s0021-9258(19)74240-9;
RA Hu Y., Kazenwadel J., James R.;
RT "Isolation and characterization of the murine homeobox gene Cdx-1.
RT Regulation of expression in intestinal epithelial cells.";
RL J. Biol. Chem. 268:27214-27225(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Embryonic intestine;
RX PubMed=2905686; DOI=10.1101/gad.2.12a.1647;
RA Duprey P., Chowdhury K., Dressler G.R., Balling R., Simon D., Guenet J.-L.,
RA Gruss P.;
RT "A mouse gene homologous to the Drosophila gene caudal is expressed in
RT epithelial cells from the embryonic intestine.";
RL Genes Dev. 2:1647-1654(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Plays a role in transcriptional regulation. Involved in
CC activated KRAS-mediated transcriptional activation of PRKD1 in
CC colorectal cancer (CRC) cells. Binds to the PRKD1 promoter in
CC colorectal cancer (CRC) cells. Could play a role in the terminal
CC differentiation of the intestine. Binds preferentially to methylated
CC DNA. {ECO:0000250|UniProtKB:P47902}.
CC -!- INTERACTION:
CC P18111; P20226: TBP; Xeno; NbExp=5; IntAct=EBI-21005290, EBI-355371;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Intestinal epithelium.
CC -!- SIMILARITY: Belongs to the Caudal homeobox family. {ECO:0000305}.
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DR EMBL; M37163; AAA37412.1; ALT_SEQ; mRNA.
DR EMBL; M80463; AAA16447.1; ALT_SEQ; Unassigned_DNA.
DR EMBL; BC019986; AAH19986.1; -; mRNA.
DR CCDS; CCDS29279.1; -.
DR PIR; A49303; A49303.
DR RefSeq; NP_034010.3; NM_009880.3.
DR AlphaFoldDB; P18111; -.
DR SMR; P18111; -.
DR BioGRID; 198663; 6.
DR IntAct; P18111; 10.
DR STRING; 10090.ENSMUSP00000025521; -.
DR PhosphoSitePlus; P18111; -.
DR PaxDb; P18111; -.
DR PeptideAtlas; P18111; -.
DR PRIDE; P18111; -.
DR ProteomicsDB; 281150; -.
DR Antibodypedia; 27929; 505 antibodies from 32 providers.
DR DNASU; 12590; -.
DR Ensembl; ENSMUST00000025521; ENSMUSP00000025521; ENSMUSG00000024619.
DR GeneID; 12590; -.
DR KEGG; mmu:12590; -.
DR UCSC; uc008fbj.2; mouse.
DR CTD; 1044; -.
DR MGI; MGI:88360; Cdx1.
DR VEuPathDB; HostDB:ENSMUSG00000024619; -.
DR eggNOG; KOG0848; Eukaryota.
DR GeneTree; ENSGT00940000162069; -.
DR HOGENOM; CLU_073177_1_0_1; -.
DR InParanoid; P18111; -.
DR OMA; DKDTNMY; -.
DR OrthoDB; 1380804at2759; -.
DR PhylomeDB; P18111; -.
DR TreeFam; TF351605; -.
DR BioGRID-ORCS; 12590; 0 hits in 75 CRISPR screens.
DR ChiTaRS; Cdx1; mouse.
DR PRO; PR:P18111; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; P18111; protein.
DR Bgee; ENSMUSG00000024619; Expressed in left colon and 39 other tissues.
DR Genevisible; P18111; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0008327; F:methyl-CpG binding; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR GO; GO:0009887; P:animal organ morphogenesis; IBA:GO_Central.
DR GO; GO:0009948; P:anterior/posterior axis specification; IBA:GO_Central.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:MGI.
DR GO; GO:0060349; P:bone morphogenesis; IMP:MGI.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0007389; P:pattern specification process; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0014807; P:regulation of somitogenesis; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR006820; Caudal_activation_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF04731; Caudal_act; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..268
FT /note="Homeobox protein CDX-1"
FT /id="PRO_0000048847"
FT DNA_BIND 154..213
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 157..178
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250|UniProtKB:P47902"
FT REGION 196..207
FT /note="Interaction with 5-mCpG DNA"
FT /evidence="ECO:0000250|UniProtKB:P47902"
FT REGION 209..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..42
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 89..109
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 224..243
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 15
FT /note="P -> Q (in Ref. 3; AAH19986)"
FT /evidence="ECO:0000305"
FT CONFLICT 146
FT /note="G -> C (in Ref. 2; AAA37412)"
FT /evidence="ECO:0000305"
FT CONFLICT 157
FT /note="Y -> S (in Ref. 2; AAA37412)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 268 AA; 28436 MW; 85F45B2A3E12B2AE CRC64;
MYVGYVLDKD SPVYPGPARP SSLGLGPPTY APPGPAPAPP QYPDFAGYTH VEPAPAPPPT
WAAPFPAPKD DWAAAYGPGP TASAASPAPL AFGPPPDFSP VPAPPGPGPG ILAQSLGAPG
APSSPGAPRR TPYEWMRRSV AAAGGGGSGK TRTKDKYRVV YTDHQRLELE KEFHYSRYIT
IRRKSELAAN LGLTERQVKI WFQNRRAKER KVNKKKQQQQ QPLPPTQLPL PLDGTPTPSG
PPLGSLCPTN AGLLGTPSPV PVKEEFLP
