CE128_HUMAN
ID CE128_HUMAN Reviewed; 1094 AA.
AC Q6ZU80; B9EK52; Q86X97; Q96ML4;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Centrosomal protein of 128 kDa;
DE Short=Cep128;
GN Name=CEP128; Synonyms=C14orf145, C14orf61;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12508121; DOI=10.1038/nature01348;
RA Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C.,
RA Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A.,
RA Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H.,
RA Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T.,
RA Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B.,
RA Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D.,
RA Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R.,
RA Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S.,
RA Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C.,
RA Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S.,
RA Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C.,
RA Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P.,
RA Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M.,
RA Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V.,
RA Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J.,
RA Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F.,
RA Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F.,
RA Waterston R., Hood L., Weissenbach J.;
RT "The DNA sequence and analysis of human chromosome 14.";
RL Nature 421:601-607(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 310-1094 (ISOFORM 2), AND
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 715-1094 (ISOFORM 1).
RC TISSUE=Placenta, and Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Lymphoblast;
RX PubMed=14654843; DOI=10.1038/nature02166;
RA Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M.;
RT "Proteomic characterization of the human centrosome by protein correlation
RT profiling.";
RL Nature 426:570-574(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=21399614; DOI=10.1038/emboj.2011.63;
RA Jakobsen L., Vanselow K., Skogs M., Toyoda Y., Lundberg E., Poser I.,
RA Falkenby L.G., Bennetzen M., Westendorf J., Nigg E.A., Uhlen M.,
RA Hyman A.A., Andersen J.S.;
RT "Novel asymmetrically localizing components of human centrosomes identified
RT by complementary proteomics methods.";
RL EMBO J. 30:1520-1535(2011).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31; SER-291; SER-331 AND
RP SER-1061, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-331, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000269|PubMed:14654843}. Cytoplasm,
CC cytoskeleton, microtubule organizing center, centrosome, centriole
CC {ECO:0000269|PubMed:21399614}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000269|PubMed:21399614}. Note=Associates with the mother
CC centriole.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=2;
CC IsoId=Q6ZU80-2; Sequence=Displayed;
CC Name=1;
CC IsoId=Q6ZU80-1; Sequence=VSP_014752, VSP_030196;
CC Name=3;
CC IsoId=Q6ZU80-3; Sequence=VSP_030194, VSP_030195;
CC -!- MISCELLANEOUS: [Isoform 1]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB71273.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC010072; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC022404; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC018513; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC045834; AAH45834.2; -; mRNA.
DR EMBL; BC150610; AAI50611.1; -; mRNA.
DR EMBL; AK056756; BAB71273.1; ALT_INIT; mRNA.
DR EMBL; AK125925; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS32130.1; -. [Q6ZU80-2]
DR RefSeq; NP_689659.2; NM_152446.3. [Q6ZU80-2]
DR RefSeq; XP_006720119.1; XM_006720056.3.
DR AlphaFoldDB; Q6ZU80; -.
DR SMR; Q6ZU80; -.
DR BioGRID; 126920; 292.
DR IntAct; Q6ZU80; 277.
DR MINT; Q6ZU80; -.
DR STRING; 9606.ENSP00000451162; -.
DR MoonDB; Q6ZU80; Predicted.
DR iPTMnet; Q6ZU80; -.
DR PhosphoSitePlus; Q6ZU80; -.
DR BioMuta; CEP128; -.
DR DMDM; 166214932; -.
DR EPD; Q6ZU80; -.
DR jPOST; Q6ZU80; -.
DR MassIVE; Q6ZU80; -.
DR MaxQB; Q6ZU80; -.
DR PaxDb; Q6ZU80; -.
DR PeptideAtlas; Q6ZU80; -.
DR PRIDE; Q6ZU80; -.
DR ProteomicsDB; 68321; -. [Q6ZU80-2]
DR ProteomicsDB; 68322; -. [Q6ZU80-1]
DR ProteomicsDB; 68323; -. [Q6ZU80-3]
DR Antibodypedia; 2; 94 antibodies from 15 providers.
DR DNASU; 145508; -.
DR Ensembl; ENST00000216517.10; ENSP00000216517.6; ENSG00000100629.17. [Q6ZU80-3]
DR Ensembl; ENST00000281129.7; ENSP00000281129.3; ENSG00000100629.17. [Q6ZU80-2]
DR Ensembl; ENST00000555265.6; ENSP00000451162.1; ENSG00000100629.17. [Q6ZU80-2]
DR GeneID; 145508; -.
DR KEGG; hsa:145508; -.
DR MANE-Select; ENST00000555265.6; ENSP00000451162.1; NM_152446.5; NP_689659.2.
DR UCSC; uc001xux.3; human. [Q6ZU80-2]
DR CTD; 145508; -.
DR DisGeNET; 145508; -.
DR GeneCards; CEP128; -.
DR HGNC; HGNC:20359; CEP128.
DR HPA; ENSG00000100629; Tissue enriched (lymphoid).
DR MalaCards; CEP128; -.
DR neXtProt; NX_Q6ZU80; -.
DR OpenTargets; ENSG00000100629; -.
DR PharmGKB; PA142672283; -.
DR VEuPathDB; HostDB:ENSG00000100629; -.
DR eggNOG; ENOG502QRR8; Eukaryota.
DR GeneTree; ENSGT00390000007020; -.
DR HOGENOM; CLU_010570_0_0_1; -.
DR InParanoid; Q6ZU80; -.
DR OMA; HTMHQSL; -.
DR OrthoDB; 160423at2759; -.
DR PhylomeDB; Q6ZU80; -.
DR TreeFam; TF331714; -.
DR PathwayCommons; Q6ZU80; -.
DR SignaLink; Q6ZU80; -.
DR BioGRID-ORCS; 145508; 7 hits in 1074 CRISPR screens.
DR ChiTaRS; CEP128; human.
DR GenomeRNAi; 145508; -.
DR Pharos; Q6ZU80; Tbio.
DR PRO; PR:Q6ZU80; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q6ZU80; protein.
DR Bgee; ENSG00000100629; Expressed in bone marrow cell and 122 other tissues.
DR ExpressionAtlas; Q6ZU80; baseline and differential.
DR Genevisible; Q6ZU80; HS.
DR GO; GO:0120103; C:centriolar subdistal appendage; IDA:GO_Central.
DR GO; GO:0005814; C:centriole; IDA:UniProtKB.
DR GO; GO:0005813; C:centrosome; IDA:HPA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000922; C:spindle pole; IDA:UniProtKB.
DR GO; GO:0008104; P:protein localization; IMP:GO_Central.
DR InterPro; IPR026652; CEP128.
DR PANTHER; PTHR46657; PTHR46657; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1094
FT /note="Centrosomal protein of 128 kDa"
FT /id="PRO_0000089946"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 115..140
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 319..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 987..1018
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1067..1094
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 190..827
FT /evidence="ECO:0000255"
FT COILED 879..959
FT /evidence="ECO:0000255"
FT COMPBIAS 1..21
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 327..341
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 31
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 249
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8BI22"
FT MOD_RES 291
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 331
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163,
FT ECO:0007744|PubMed:24275569"
FT MOD_RES 1061
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 404..435
FT /note="NLTRELENGEKQQLQMLDRLKEIQNHFDTCEA -> HINRKCLLNLVQDLDC
FT KDNEILTYSLQSPLHV (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_030194"
FT VAR_SEQ 436..1094
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_030195"
FT VAR_SEQ 987
FT /note="D -> R (in isoform 1)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_014752"
FT VAR_SEQ 988..1094
FT /note="Missing (in isoform 1)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_030196"
FT VARIANT 16
FT /note="R -> L (in dbSNP:rs7160694)"
FT /id="VAR_037835"
FT VARIANT 732
FT /note="H -> R (in dbSNP:rs327463)"
FT /id="VAR_037836"
FT CONFLICT 395
FT /note="K -> R (in Ref. 2; AAH45834)"
FT /evidence="ECO:0000305"
FT CONFLICT 973
FT /note="P -> A (in Ref. 3; BAB71273)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1094 AA; 128015 MW; BDE05923E3F6B0FF CRC64;
MAESSSESDH FRCRDRLSPW AARSTHRGTR SLPTVEVTEK VNTITSTLQD TSRNLRQVDQ
MLGRYREYSN GQAGAIEHLK ESLEQSIDQL RSQRLLRNSG GRSISVTSLS ASDLDGGTGS
ELHHFPPTSP LKDYGDPQGI KRMRSRTGVR FVQETDDMTQ LHGFHQSLRD LSSEQIRLGD
DFNRELSRRS RSDAETKRAL EELTEKLNEA QKQEVVSDRV ERRLQELERE MRTERELVER
RQDQLGLMSL QLQEALKKQE AKADEHEGAI KNKLRQTETE KNQLEQELEL SRRLLNQSEG
SRETLLHQVE ELRTQLTKAE GDRKGLQHQV SQISKQQSNY QDEQGEDWRF RRGVEREKQD
LEKQMSDLRV QLNFSAMASE LEEVKRCMER KDKEKAHLAS QVENLTRELE NGEKQQLQML
DRLKEIQNHF DTCEAERKHA DLQISELTRH AEDATKQAER YLSELQQSEA LKEEAEKRRE
DLKLKAQESI RQWKLKHKKL ERALEKQSET VDELTGKNNQ ILKEKDELKT QLYAALQQIE
NLRKELNDVL TKRALQEEEL HSKEEKLRDI KSHQADLELE VKNSLDTIHR LESELKKQSK
IQSQMKVEKA HLEEEIAELK KSQAQDKAKL LEMQESIKDL SAIRADLANK LAEEERAKKA
VLKDLSDLTA QAKSRDEETA TIITQLKLER DVHQRELKDL TSSLQSVKTK HEQNIQELMK
HFKKEKSEAE NHIRTLKAES LEEKNMAKIH RGQLEKLKSQ CDRLTEELTQ NENENKKLKL
KYQCLKDQLE EREKHISIEE EHLRRMEEAR LQLKDQLLCL ETEQESILGV IGKEIDAACK
TFSKDSVEKL KVFSSGPDIH YDPHRWLAES KTKLQWLCEE LKERENREKN LRHQLMLCRQ
QLRNLTENKE SELQCLFQQI ERQEQLLDEI HREKRDLLEE TQRKDEEMGS LQDRVIALET
STQVALDHLE SVPEKLSLLE DFKDFRDSCS SSERTDGRYS KYRVRRNSLQ HHQDDTKYRT
KSFKGDRTFL EGSHTRGLDH SSSWQDHSRF LSSPRFSYVN SFTKRTVAPD SASNKEDATM
NGTSSQPKKE EYGS
