CE128_MOUSE
ID CE128_MOUSE Reviewed; 1102 AA.
AC Q8BI22;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Centrosomal protein of 128 kDa;
DE Short=Cep128;
GN Name=Cep128;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 454-1102 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-248, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome, centriole {ECO:0000250}. Cytoplasm, cytoskeleton,
CC spindle pole {ECO:0000250}. Note=Associates with the mother centriole.
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BI22-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BI22-2; Sequence=VSP_030197, VSP_030198;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC26523.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; BC079647; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK029581; BAC26523.1; ALT_INIT; mRNA.
DR CCDS; CCDS36515.2; -. [Q8BI22-1]
DR RefSeq; NP_861536.3; NM_181815.3. [Q8BI22-1]
DR AlphaFoldDB; Q8BI22; -.
DR SMR; Q8BI22; -.
DR STRING; 10090.ENSMUSP00000115679; -.
DR iPTMnet; Q8BI22; -.
DR PhosphoSitePlus; Q8BI22; -.
DR EPD; Q8BI22; -.
DR jPOST; Q8BI22; -.
DR MaxQB; Q8BI22; -.
DR PaxDb; Q8BI22; -.
DR PRIDE; Q8BI22; -.
DR ProteomicsDB; 280047; -. [Q8BI22-1]
DR ProteomicsDB; 280048; -. [Q8BI22-2]
DR Antibodypedia; 2; 94 antibodies from 15 providers.
DR Ensembl; ENSMUST00000141429; ENSMUSP00000115679; ENSMUSG00000061533. [Q8BI22-1]
DR GeneID; 75216; -.
DR KEGG; mmu:75216; -.
DR UCSC; uc007okl.2; mouse. [Q8BI22-1]
DR CTD; 145508; -.
DR MGI; MGI:1922466; Cep128.
DR VEuPathDB; HostDB:ENSMUSG00000061533; -.
DR eggNOG; ENOG502QRR8; Eukaryota.
DR GeneTree; ENSGT00390000007020; -.
DR HOGENOM; CLU_010570_0_0_1; -.
DR InParanoid; Q8BI22; -.
DR OMA; HTMHQSL; -.
DR OrthoDB; 160423at2759; -.
DR PhylomeDB; Q8BI22; -.
DR TreeFam; TF331714; -.
DR BioGRID-ORCS; 75216; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Cep128; mouse.
DR PRO; PR:Q8BI22; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q8BI22; protein.
DR Bgee; ENSMUSG00000061533; Expressed in spermatid and 206 other tissues.
DR ExpressionAtlas; Q8BI22; baseline and differential.
DR Genevisible; Q8BI22; MM.
DR GO; GO:0120103; C:centriolar subdistal appendage; ISO:MGI.
DR GO; GO:0005814; C:centriole; ISS:UniProtKB.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000922; C:spindle pole; ISS:UniProtKB.
DR GO; GO:0008104; P:protein localization; ISO:MGI.
DR InterPro; IPR026652; CEP128.
DR PANTHER; PTHR46657; PTHR46657; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1102
FT /note="Centrosomal protein of 128 kDa"
FT /id="PRO_0000089947"
FT REGION 326..346
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 991..1048
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1070..1102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 215..822
FT /evidence="ECO:0000255"
FT COILED 878..959
FT /evidence="ECO:0000255"
FT COMPBIAS 1006..1032
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1072..1088
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 31
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6ZU80"
FT MOD_RES 248
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 290
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6ZU80"
FT VAR_SEQ 1024..1026
FT /note="DDR -> VST (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030197"
FT VAR_SEQ 1027..1102
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030198"
SQ SEQUENCE 1102 AA; 128503 MW; 47108A8211B69939 CRC64;
MAESSSDSDH VRYRDGLSRW ATRSFQRGVG SHSTVDVTAK VNTITNTLQD TTRNLRHVDQ
MLGRYREYST GQAGAVGQLK EDLEQSINQL RSQRLRRSSG GRSVSVTSLS ASDLDGGAVT
ENLRFAPTSP LKDYDLQGIK RNRFRTGVRF VSETDDMVQL HTFHQSLRDL SSEQVRLGDD
LNRELFRRSR SDAETKRVLE ELTGKLNEVQ KPDVVSDRVE RRLQEIEREM RLERELVERR
HDQLGLVSLK LQEALKKQEA KADENEDVIK RKLRQSETEK SQLEQELEIS RRLLSQSESN
RETLLHQVEE LRTQLIKAEG DQRGLQHQVP CISKQPLSHQ DDQGDDRRFR RGVEREKLSL
EKQMADLRVQ LNFNSMASEL EEVKRCMERK DQEKATLAAQ IENLTRDLEN REKQQLQMLD
QLTEIQNHFE TCEANRKRTD LQLSELSQHA EEATKQAEHY LSEFQRSETL REEAEKRRED
LKAKAQESIR QWKLKHKKLE RSMEKQAETL VQLTEKNNQF IKERDELKSQ LCAALQQIEN
LRKELNDVLS KRALQEEELH CKEKKLNDIE SHQAELELEV KNSLDTIHRL ENELKRQSKS
QSQIKAEKIH LEEEITELKK SQSQDKVKLL EMQESIKDLS AIRADLANKL AEEEKAKKAV
FRDLSELTAQ VKSKEEETAT AITQLKLERD VHQRELEDLS SSLESVKLKH EQNIQELMKH
FKKEKSEAES HIRMLKAESL EDKNMAKAHL GQLEKLKSQC EKLTEELTHT ENENKKLKLK
YQSLKEELDK KEKYISTEEE HLRRMEESRL HLKDQLLCLE TEQESILGVI GKEIDEACKT
FSRDSLEKLK VLTSGPQLHY DPHRWLAESK TKLQWLCEEL KERESRERSM RQQLAACRQE
LRELTEHKES ELLCLFEHIE RQEQLLEEFH QEKRGLLEET QRKDEEVETL QDRVNALQMS
TRVALDHLES VPEKLSLLED FKDFRGASSL SEKTDGRYSK YSLHGDSVQQ RRDDTKPRIK
SFRDDRPLSA GSHAHGLDHS SSCQDHSRFL SSPQFSHSLP VFTKRTIATD PASIEGDTTS
LPANGTSPQS KKEEHEIKKY KK
