CE162_RAT
ID CE162_RAT Reviewed; 1403 AA.
AC Q4KLH6;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Centrosomal protein of 162 kDa;
DE Short=Cep162;
DE AltName: Full=Protein QN1 homolog;
GN Name=Cep162; Synonyms=Qn1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH ALPHA-TUBULIN.
RX PubMed=16302001; DOI=10.1038/sj.onc.1209215;
RA Leon A., Omri B., Gely A., Klein C., Crisanti P.;
RT "QN1/KIAA1009: a new essential protein for chromosome segregation and
RT mitotic spindle assembly.";
RL Oncogene 25:1887-1895(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156 AND SER-159, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Required to promote assembly of the transition zone in
CC primary cilia. Acts by specifically recognizing and binding the
CC axonemal microtubule. Localizes to the distal ends of centrioles before
CC ciliogenesis and directly binds to axonemal microtubule, thereby
CC promoting and restricting transition zone formation specifically at the
CC cilia base. Required to mediate CEP290 association with microtubules
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with alpha-tubulin (PubMed:16302001). Interacts with
CC CPNE4 (By similarity). Interacts with CEP290 (By similarity).
CC {ECO:0000250|UniProtKB:Q5TB80, ECO:0000250|UniProtKB:Q6ZQ06,
CC ECO:0000269|PubMed:16302001}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome, centriole {ECO:0000250}. Cytoplasm, cytoskeleton,
CC spindle {ECO:0000269|PubMed:16302001}. Nucleus {ECO:0000250}.
CC Note=Localizes to the distal end of centrioles throughout the cell
CC cycle. During ciliogenesis, found at the cilia base. Localizes to
CC spindle microtubules during mitosis (By similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q4KLH6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q4KLH6-2; Sequence=VSP_026958, VSP_026959;
CC -!- SIMILARITY: Belongs to the CEP162 family. {ECO:0000305}.
CC -!- CAUTION: Was initially thought to regulate chromosome segregation and
CC mitotic spindle assembly (PubMed:16302001). However, it was later shown
CC that its absence neither affect mitosis nor centriole duplication.
CC {ECO:0000305|PubMed:16302001}.
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DR EMBL; AABR03063564; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03063586; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03063843; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC099201; AAH99201.1; -; mRNA.
DR RefSeq; NP_001263989.1; NM_001277060.1. [Q4KLH6-1]
DR RefSeq; XP_006243537.1; XM_006243475.3. [Q4KLH6-1]
DR AlphaFoldDB; Q4KLH6; -.
DR SMR; Q4KLH6; -.
DR BioGRID; 256747; 1.
DR STRING; 10116.ENSRNOP00000014165; -.
DR iPTMnet; Q4KLH6; -.
DR PhosphoSitePlus; Q4KLH6; -.
DR PaxDb; Q4KLH6; -.
DR PRIDE; Q4KLH6; -.
DR Ensembl; ENSRNOT00000014165; ENSRNOP00000014165; ENSRNOG00000010608. [Q4KLH6-1]
DR GeneID; 300880; -.
DR KEGG; rno:300880; -.
DR UCSC; RGD:1307365; rat. [Q4KLH6-1]
DR CTD; 22832; -.
DR RGD; 1307365; Cep162.
DR eggNOG; ENOG502QSPF; Eukaryota.
DR GeneTree; ENSGT00390000009631; -.
DR HOGENOM; CLU_005179_0_0_1; -.
DR InParanoid; Q4KLH6; -.
DR OMA; NESRGFD; -.
DR OrthoDB; 520583at2759; -.
DR PhylomeDB; Q4KLH6; -.
DR TreeFam; TF330884; -.
DR Reactome; R-RNO-5620912; Anchoring of the basal body to the plasma membrane.
DR PRO; PR:Q4KLH6; -.
DR Proteomes; UP000002494; Chromosome 8.
DR Bgee; ENSRNOG00000010608; Expressed in thymus and 19 other tissues.
DR Genevisible; Q4KLH6; RN.
DR GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR GO; GO:0034451; C:centriolar satellite; IEA:Ensembl.
DR GO; GO:0005814; C:centriole; ISS:UniProtKB.
DR GO; GO:0005813; C:centrosome; ISO:RGD.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR InterPro; IPR038774; CEP162-like.
DR PANTHER; PTHR34031; PTHR34031; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cilium biogenesis/degradation; Coiled coil;
KW Cytoplasm; Cytoskeleton; Microtubule; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1403
FT /note="Centrosomal protein of 162 kDa"
FT /id="PRO_0000295630"
FT REGION 19..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 171..235
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 305..342
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 449..586
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 610..1120
FT /evidence="ECO:0000255"
FT COILED 1170..1205
FT /evidence="ECO:0000255"
FT COILED 1234..1385
FT /evidence="ECO:0000255"
FT COMPBIAS 177..192
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 449..472
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 500..524
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 533..549
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 156
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 159
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 468
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5TB80"
FT VAR_SEQ 1..1187
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026958"
FT VAR_SEQ 1188..1208
FT /note="EREKLKMESEAAVCQLESSMK -> MCGPPELRECEPLSMLSCVCP (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026959"
SQ SEQUENCE 1403 AA; 161001 MW; E13F5DF8C23B658E CRC64;
MAHYSKVDLD EEFERFMKEL SDDSFENSNE TPSQPNKDRK KKDTAPWWIA EDGFEDDGLL
GTNVSYLKTK KTYQPITDME EENEKVQFLK SSGTSVLSVD SLEANELVAS ELHHSTLGLG
LDTLEEQEEK EQFFARLEKG LTSSIDYSKL NQELDSDDSA QFRVLHRYQG NVEPAEGGRE
NESEHKELPE TYSDDFEDAE DTDEPLITKD EETRPKENPE SGKGSFPNQE EEKTGMLANV
VLLDSFDSVE DVDLNNHERP TPKAKALPEM AGGELAETGV SYGQSSGDTE ALHQAYHHVA
HSLGDTGEPR IEASPGHSVR SSAKDGLQEN EESSKNISTT ESDLPTVEEL MKPIRIDSYG
IRGFDLQPVS LQKAVGSKEA ESVSSLPLTV NTNTMSQDTR HVSPFPHEQD ESVVLHRTAG
EGVGSSCPAT EEHLDKMYLE ILRKKTSVNP SLLPQDNKAN QTSRSRLSAR EEAPVTSKQV
PCKKARSAPP LPRRKPQSGL YASARSSGYS KPSSPLQFFS ALEKKTSKDN TKTKNVRPIP
TSNQFRKREI LSGTKLIKPA ALNKPSPHRE GSPATPKRPE DPSDDSFVQL QTETLGSYGG
NREKELLMLK RAQDAEEKWR GAQALIEQIK MTFSEKEKEL ENTVESLKRQ QERELFKLNQ
ENYILQAKLS SFEETSRKQR WLQFGETSDP LTEEKLKQIQ KEIQEQETLL QGYQQENERL
YSQVKDLQEQ NKKNEERMFK ENQNLFSELA SLKEQMHKNH FLPQAVENIE PTKNQSFTDL
LAELRAAQKE KNHLMEDIKR LKQDKQALEV DLERVKRERD QAKDQIAYTT GEKLYEIKIL
EETHKQEVSR LQKRLQWYAE NQELLDKDAA RLREANEETE RLKLEIEKLK TESGSPANQQ
RLRSKERALD AKRIQDLERQ VKEMEGILKR RYPNSLPALI LAASAAGDSV DRNTVDFMER
RIKKLEADLE GKDEEAKKSL RTMEQQFQKM KIQYEQRLEE QEQMLAHRQR EAPQNQHNSS
SRLKALETEL GGIKEAHQIT VRKLEAEIDV LKHQNAHLEH KKNDKEDQDL QSIEFQVEQA
QARAKLARLN EELAAKGREI QDLTKTVERL QKERRMMLSR QSPRGREEMA AKRLKKEILH
PNSGNANAFS ETLGAKLYHP HTFTDSHISE VLEENYRLRS ELEGLTLERE KLKMESEAAV
CQLESSMKRV KDDAAAHIAS LKAAHEREIE KLLCQNAVEN SSSRVAELNR KIATQEVLLK
HFQGQVNELQ GKQESLSLSQ VREEILQKQI TKLLEELKEA KENHTPEMKH FMGLERKIKQ
MEMRHKQREQ ELQQIIQQTR QVVETEQNKE VEKWKRLAQL KNRELDKFRT ELDSILDVLR
ELHRQGVVVP VALADEESTA KEF
