ID   CEA5_ECOLX              Reviewed;         180 AA.
AC   P18000;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Colicin-E5;
DE            EC=3.1.-.-;
DE   Flags: Fragment;
GN   Name=col;
OS   Escherichia coli.
OG   Plasmid ColE5-099.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2549375; DOI=10.1007/bf02464892;
RA   Lau P.C.K., Condie J.A.;
RT   "Nucleotide sequences from the colicin E5, E6 and E9 operons: presence of a
RT   degenerate transposon-like structure in the ColE9-J plasmid.";
RL   Mol. Gen. Genet. 217:269-277(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-180.
RX   PubMed=2561131; DOI=10.1099/00221287-135-10-2783;
RA   Curtis M.D., James R., Coddington A.;
RT   "An evolutionary relationship between the ColE5-099 and the ColE9-J
RT   plasmids revealed by nucleotide sequencing.";
RL   J. Gen. Microbiol. 135:2783-2788(1989).
CC   -!- FUNCTION: Colicins are polypeptide toxins produced by and active
CC       against E.coli and closely related bacteria. This colicin is an
CC       endonuclease.
CC   -!- SIMILARITY: Belongs to the colicin/pyosin nuclease family.
CC       {ECO:0000305}.
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DR   EMBL; X15857; CAA33859.1; -; Genomic_DNA.
DR   EMBL; M30445; AAA98051.1; -; Genomic_DNA.
DR   PIR; A45799; A45799.
DR   PIR; PQ0031; PQ0031.
DR   PDB; 2A8K; X-ray; 1.50 A; A/B/C/D=74-180.
DR   PDB; 2DFX; X-ray; 1.90 A; E=66-180.
DR   PDB; 2DJH; X-ray; 1.90 A; A=66-180.
DR   PDB; 2FHZ; X-ray; 1.15 A; B=74-180.
DR   PDB; 3AO9; X-ray; 2.10 A; A/B=66-180.
DR   PDB; 3VJ7; X-ray; 2.30 A; A=66-180.
DR   PDBsum; 2A8K; -.
DR   PDBsum; 2DFX; -.
DR   PDBsum; 2DJH; -.
DR   PDBsum; 2FHZ; -.
DR   PDBsum; 3AO9; -.
DR   PDBsum; 3VJ7; -.
DR   AlphaFoldDB; P18000; -.
DR   SMR; P18000; -.
DR   EvolutionaryTrace; P18000; -.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.2310.30; -; 1.
DR   InterPro; IPR038233; Colicin_D/E5_nuclease.
DR   InterPro; IPR021964; Colicin_E5_C.
DR   InterPro; IPR038234; Colicin_E5_C_sf.
DR   Pfam; PF12106; Colicin_E5; 1.
DR   SUPFAM; SSF102824; SSF102824; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Bacteriocin; Endonuclease;
KW   Hydrolase; Nuclease; Plasmid.
FT   CHAIN           <1..180
FT                   /note="Colicin-E5"
FT                   /id="PRO_0000218681"
FT   REGION          24..143
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          74..180
FT                   /note="Nuclease"
FT                   /evidence="ECO:0000255"
FT   REGION          155..180
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        51..103
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        104..120
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        57
FT                   /note="K -> R (in Ref. 2; AAA98051)"
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
FT   HELIX           88..93
FT                   /evidence="ECO:0007829|PDB:2FHZ"
FT   HELIX           94..96
FT                   /evidence="ECO:0007829|PDB:2FHZ"
FT   HELIX           101..109
FT                   /evidence="ECO:0007829|PDB:2FHZ"
FT   STRAND          114..120
FT                   /evidence="ECO:0007829|PDB:2FHZ"
FT   HELIX           122..124
FT                   /evidence="ECO:0007829|PDB:2FHZ"
FT   TURN            126..128
FT                   /evidence="ECO:0007829|PDB:2FHZ"
FT   STRAND          132..141
FT                   /evidence="ECO:0007829|PDB:2FHZ"
FT   STRAND          144..149
FT                   /evidence="ECO:0007829|PDB:2FHZ"
FT   TURN            150..152
FT                   /evidence="ECO:0007829|PDB:2FHZ"
FT   STRAND          154..158
FT                   /evidence="ECO:0007829|PDB:2FHZ"
FT   STRAND          173..175
FT                   /evidence="ECO:0007829|PDB:2A8K"
SQ   SEQUENCE   180 AA;  19874 MW;  9DE1713F474B2BA4 CRC64;
     RFAHDPMAGG HRMWQMAGLK AQRAQTDVNN KQAAFDAAAK EKADADAALS TAMESRKKKE
     DNKRDAEGKL NDELAKNKGK IPGLKIDQKI RGQMPERGWT EDDIKNTVSN GATGTSFDKR
     SPKKTPPDYL GRNDPATVYG SPGKYVVVND RTGEVTQISD KTDPGWVDDS RIQWGNKNDQ