ID   CEA6_ECOLX              Reviewed;         551 AA.
AC   P17999;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Colicin-E6;
DE            EC=3.1.-.-;
DE   AltName: Full=Ribonuclease;
OS   Escherichia coli.
OG   Plasmid ColE6-CT14.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2687234; DOI=10.1128/jb.171.12.6430-6436.1989;
RA   Akutsu A., Masaki H., Ohta T.;
RT   "Molecular structure and immunity specificity of colicin E6, an
RT   evolutionary intermediate between E-group colicins and cloacin DF13.";
RL   J. Bacteriol. 171:6430-6436(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 377-551.
RX   PubMed=2549375; DOI=10.1007/bf02464892;
RA   Lau P.C.K., Condie J.A.;
RT   "Nucleotide sequences from the colicin E5, E6 and E9 operons: presence of a
RT   degenerate transposon-like structure in the ColE9-J plasmid.";
RL   Mol. Gen. Genet. 217:269-277(1989).
CC   -!- FUNCTION: Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes
CC       at a specific site.
CC   -!- FUNCTION: Colicins are polypeptide toxins produced by and active
CC       against E.coli and closely related bacteria.
CC   -!- SIMILARITY: Belongs to the cloacin colicin family. {ECO:0000305}.
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DR   EMBL; X15856; CAA33855.1; -; Genomic_DNA.
DR   EMBL; M31808; AAA23080.1; -; Genomic_DNA.
DR   PIR; PQ0030; PQ0030.
DR   AlphaFoldDB; P17999; -.
DR   SMR; P17999; -.
DR   GO; GO:0005727; C:extrachromosomal circular DNA; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0043022; F:ribosome binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.380.10; -; 1.
DR   InterPro; IPR024575; Cloacin_colicin_fam.
DR   InterPro; IPR036725; ColE3_ribonuclease_sf.
DR   InterPro; IPR009105; Colicin_E3_ribonuclease.
DR   InterPro; IPR024566; Colicin_R_dom.
DR   InterPro; IPR016128; Pyosin/cloacin_T_dom.
DR   InterPro; IPR036302; Pyosin/cloacin_T_dom_sf.
DR   Pfam; PF03515; Cloacin; 1.
DR   Pfam; PF09000; Cytotoxic; 1.
DR   Pfam; PF11570; E2R135; 1.
DR   PRINTS; PR01295; CLOACIN.
DR   SUPFAM; SSF63840; SSF63840; 1.
DR   SUPFAM; SSF69369; SSF69369; 1.
PE   3: Inferred from homology;
KW   Antibiotic; Antimicrobial; Bacteriocin; Endonuclease; Hydrolase; Nuclease;
KW   Plasmid.
FT   CHAIN           1..551
FT                   /note="Colicin-E6"
FT                   /id="PRO_0000218678"
FT   REGION          1..74
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          244..269
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          293..317
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          406..501
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          455..551
FT                   /note="Ribosome inactivating activity"
FT   REGION          517..551
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          530..551
FT                   /note="Binding of immunity protein"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        9..23
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        295..317
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        427..488
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   551 AA;  58011 MW;  D223D7F0770392E0 CRC64;
     MSGGDGRGHN TGAHSTSGNI NGGPTGLGVG GGASDGSGWS SENNPWGGGS GSGIHWGGGS
     GHGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA GGLAVSISAG ALSAAIADIM
     AALKGPFKFG LWGVALYGVL PSQIAKDDPN MMSKIVTSLP ADDITESPVS SLPLDKATVN
     VNVRVVDDVK DERQNISVVS GVPMSVPVVD AKPTERPGVF TASIPGAPVL NISVNNSTPA
     VQTLSPGVTN NTDKDVRPAG FTQGGNTRDA VIRFPKDSGH NAVYVSVSDV LSPDQVKQRQ
     DEENRRQQEW DATHPVEAAE RNYERARAEL NQANEDVARN QERQAKAVQV YNSRKSELDA
     ANKTLADAIA EIKQFNRFAH DPMAGGHRMW QMAGLKAQRA QTDVNNKQAA FDAAAKEKSD
     ADAALSSAME SRKKKEDKKR SAENKLNEEK NKPRKGVKDY GHDYHPDPKT EDIKGLGELK
     EGKPKTPKQG GGGKRARWYG DKGRKIYEWD SQHGELEGYR ASDGQHLGSF EPKTGNQLKG
     PDPKRNIKKY L