ID   CEAD_ECOLX              Reviewed;         697 AA.
AC   P17998;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Colicin-D;
GN   Name=cda;
OS   Escherichia coli.
OG   Plasmid ColD-CA23.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2677603; DOI=10.1111/j.1365-2958.1989.tb00238.x;
RA   Roos U., Harkness R.E., Braun V.;
RT   "Assembly of colicin genes from a few DNA fragments. Nucleotide sequence of
RT   colicin D.";
RL   Mol. Microbiol. 3:891-902(1989).
CC   -!- FUNCTION: Colicins are polypeptide toxins produced by and active
CC       against E.coli and closely related bacteria.
CC   -!- FUNCTION: Colicin D inhibits protein synthesis.
CC   -!- INTERACTION:
CC       P17998; P11899: cdi; NbExp=3; IntAct=EBI-1038167, EBI-1038162;
CC   -!- MISCELLANEOUS: This colicin requires TonB for its uptake.
CC   -!- SIMILARITY: Belongs to the cloacin colicin family. {ECO:0000305}.
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DR   EMBL; X14941; CAA33072.1; -; Genomic_DNA.
DR   PIR; S09254; S09254.
DR   RefSeq; WP_016245160.1; NZ_LM996936.1.
DR   PDB; 1TFK; X-ray; 2.10 A; A=604-697.
DR   PDB; 1TFO; X-ray; 2.30 A; A=595-697.
DR   PDB; 1V74; X-ray; 2.00 A; A=591-697.
DR   PDB; 5ZNM; X-ray; 1.85 A; A/B=313-697.
DR   PDBsum; 1TFK; -.
DR   PDBsum; 1TFO; -.
DR   PDBsum; 1V74; -.
DR   PDBsum; 5ZNM; -.
DR   AlphaFoldDB; P17998; -.
DR   SMR; P17998; -.
DR   IntAct; P17998; 1.
DR   EvolutionaryTrace; P17998; -.
DR   GO; GO:0005727; C:extrachromosomal circular DNA; IEA:InterPro.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.450.200; -; 1.
DR   InterPro; IPR024575; Cloacin_colicin_fam.
DR   InterPro; IPR038233; Colicin_D/E5_nuclease.
DR   InterPro; IPR024440; ColicinD_C.
DR   InterPro; IPR037178; ColicinD_C_sf.
DR   InterPro; IPR016128; Pyosin/cloacin_T_dom.
DR   InterPro; IPR036302; Pyosin/cloacin_T_dom_sf.
DR   Pfam; PF03515; Cloacin; 1.
DR   Pfam; PF11429; Colicin_D; 1.
DR   Pfam; PF06958; Pyocin_S; 1.
DR   PRINTS; PR01295; CLOACIN.
DR   SUPFAM; SSF102824; SSF102824; 1.
DR   SUPFAM; SSF69369; SSF69369; 2.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Bacteriocin; Plasmid; TonB box.
FT   CHAIN           1..697
FT                   /note="Colicin-D"
FT                   /id="PRO_0000218688"
FT   MOTIF           17..24
FT                   /note="TonB box"
FT   HELIX           313..332
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   HELIX           342..352
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          359..361
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          366..371
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   TURN            372..374
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          375..381
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   HELIX           385..388
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   HELIX           389..396
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          406..413
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          436..442
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   HELIX           443..445
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          457..460
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          462..470
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          473..480
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          482..486
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          488..492
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   TURN            499..501
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          504..508
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          517..522
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   HELIX           566..568
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          572..577
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          581..592
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          597..599
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   HELIX           602..607
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   HELIX           609..615
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   HELIX           624..639
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          656..659
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   TURN            661..663
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          665..669
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   STRAND          675..680
FT                   /evidence="ECO:0007829|PDB:5ZNM"
FT   HELIX           686..694
FT                   /evidence="ECO:0007829|PDB:5ZNM"
SQ   SEQUENCE   697 AA;  74688 MW;  F468B4A0172ABBB1 CRC64;
     MSDYEGSGPT EGIDYGHSMV VWPSTGLISG GDVKPGGSSG IAPSMPPGWG DYSPQGIALV
     QSVLFPGIIR RIILDKELEE GDWSGWSVSV HSPWGNEKVS AARTVLENGL RGGLPEPSRP
     AAVSFARLEP ASGNEQKIIR LMVTQQLEQV TDIPASQLPA AGNNVPVKYR LMDLMQNGTQ
     YMAIIGGIPM TVPVVDAVPV PDRSRPGTNI KDVYSAPVSP NLPDLVLSVG QMNTPVLSNP
     EIQEEGVIAE TGNYVEAGYT MSSNNHDVIV RFPEGSDVSP LYISTVEILD SNGLSQRQEA
     ENKAKDDFRV KKEEAVARAE AEKAKAELFS KAGVNQPPVY TQEMMERANS VMNEQGALVL
     NNTASSVQLA MTGTGVWTAA GDIAGNISKF FSNALEKVTI PEVSPLLMRI SLGALWFHSE
     EAGAGSDIVP GRNLEAMFSL SAQMLAGQGV VIEPGATSVN LPVRGQLINS NGQLALDLLK
     TGNESIPAAV PVLNAVRDTA TGLDKITLPA VVGAPSRTIL VNPVPQPSVP TDTGNHQPVP
     VTPVHTGTEV KSVEMPVTTI TPVSDVGGLR DFIYWRPDAA GTGVEAVYVM LNDPLDSGRF
     SRKQLDKKYK HAGDFGISDT KKNRETLTKF RDAIEEHLSD KDTVEKGTYR REKGSKVYFN
     PNTMNVVIIK SNGEFLSGWK INPDADNGRI YLETGEL