CEAM7_HUMAN
ID CEAM7_HUMAN Reviewed; 265 AA.
AC Q14002; A8K848; O15148; O15149; Q0VAC1; Q13983; Q9UPJ2;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 23-FEB-2022, sequence version 2.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Carcinoembryonic antigen-related cell adhesion molecule 7 {ECO:0000305};
DE Short=CEA cell adhesion molecule 7;
DE AltName: Full=Carcinoembryonic antigen CGM2;
DE Flags: Precursor;
GN Name=CEACAM7 {ECO:0000312|HGNC:HGNC:1819}; Synonyms=CGM2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2A), AND VARIANT PHE-120.
RX PubMed=7806520; DOI=10.1016/s0021-9258(20)30079-x;
RA Thompson J., Zimmermann W., Nollau P., Neumaier M., Weber-Arden J.,
RA Schrewe H., Craig I., Willcocks T.;
RT "CGM2, a member of the carcinoembryonic antigen gene family is down-
RT regulated in colorectal carcinomas.";
RL J. Biol. Chem. 269:32924-32931(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2A), AND VARIANT PHE-120.
RC TISSUE=Colon mucosa;
RX PubMed=9135022;
RA Thompson J., Seitz M., Chastre E., Ditter M., Aldrian C., Gespach C.,
RA Zimmermann W.;
RT "Down-regulation of carcinoembryonic antigen family member 2 expression is
RT an early event in colorectal tumorigenesis.";
RL Cancer Res. 57:1776-1784(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 2A AND 2B), AND VARIANT
RP PHE-120.
RA Zhou G.Q.;
RT "Two isoforms of CEA gene family member 2 (CGM2) mRNA are co-expressed in
RT small and large intestine mucosa epithelium and in colorectal tumor
RT cells.";
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2A).
RC TISSUE=Colon, and Esophagus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT PHE-120.
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2A).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 23-142.
RC TISSUE=Liver;
RX PubMed=2537643; DOI=10.1016/0006-291x(89)92821-0;
RA Thompson J.A., Mauch E.-M., Chen F.-S., Hinoda Y., Schrewe H., Berling B.,
RA Barnert S., von Kleist S., Shively J.E., Zimmermann W.;
RT "Analysis of the size of the carcinoembryonic antigen (CEA) gene family:
RT isolation and sequencing of N-terminal domain exons.";
RL Biochem. Biophys. Res. Commun. 158:996-1004(1989).
RN [8]
RP NUCLEOTIDE SEQUENCE OF 35-142, AND VARIANT PHE-120.
RX PubMed=1427854; DOI=10.1016/s0888-7543(05)80230-7;
RA Khan W.N., Fraengsmyr L., Teglund S., Israelsson A., Bremer K.,
RA Hammarstroem S.;
RT "Identification of three new genes and estimation of the size of the
RT carcinoembryonic antigen family.";
RL Genomics 14:384-390(1992).
RN [9]
RP PROTEIN SEQUENCE OF 36-50.
RX PubMed=15340161; DOI=10.1110/ps.04682504;
RA Zhang Z., Henzel W.J.;
RT "Signal peptide prediction based on analysis of experimentally verified
RT cleavage sites.";
RL Protein Sci. 13:2819-2824(2004).
RN [10]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=10436421; DOI=10.1159/000030075;
RA Fraengsmyr L., Baranov V., Hammarstroem S.;
RT "Four carcinoembryonic antigen subfamily members, CEA, NCA, BGP and CGM2,
RT selectively expressed in the normal human colonic epithelium, are integral
RT components of the fuzzy coat.";
RL Tumor Biol. 20:277-292(1999).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (1.47 ANGSTROMS) OF 34-142, AND SUBUNIT.
RX PubMed=26323304; DOI=10.1107/s2053230x15013576;
RA Bonsor D.A., Beckett D., Sundberg E.J.;
RT "Structure of the N-terminal dimerization domain of CEACAM7.";
RL Acta Crystallogr. F 71:1169-1175(2015).
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:26323304}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000305}. Apical cell membrane
CC {ECO:0000269|PubMed:10436421}. Note=Localized to the apical glycocalyx
CC surface. {ECO:0000269|PubMed:10436421}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=2a;
CC IsoId=Q14002-1; Sequence=Displayed;
CC Name=2b;
CC IsoId=Q14002-2; Sequence=VSP_002488;
CC -!- TISSUE SPECIFICITY: Expressed in columnar epithelial cells of the colon
CC (at protein level) (PubMed:10436421). Strongly down-regulated in
CC colonic adenocarcinomas. {ECO:0000269|PubMed:10436421}.
CC -!- SIMILARITY: Belongs to the immunoglobulin superfamily. CEA family.
CC {ECO:0000305}.
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DR EMBL; L31792; AAA66186.1; -; mRNA.
DR EMBL; X98311; CAA66955.1; -; mRNA.
DR EMBL; AF006622; AAB62924.1; -; mRNA.
DR EMBL; AF006623; AAB62925.1; -; mRNA.
DR EMBL; AK292213; BAF84902.1; -; mRNA.
DR EMBL; AK313108; BAG35930.1; -; mRNA.
DR EMBL; AC005797; AAC62825.1; -; Genomic_DNA.
DR EMBL; AC005797; AAC62826.1; -; Genomic_DNA.
DR EMBL; BC121132; AAI21133.1; -; mRNA.
DR EMBL; M22434; AAA51966.1; -; Genomic_DNA.
DR CCDS; CCDS12583.1; -. [Q14002-1]
DR PIR; A55811; A55811.
DR RefSeq; NP_001278414.1; NM_001291485.1.
DR RefSeq; NP_008821.2; NM_006890.4.
DR PDB; 4Y89; X-ray; 1.47 A; A/B/C/D=34-142.
DR PDBsum; 4Y89; -.
DR AlphaFoldDB; Q14002; -.
DR BioGRID; 107513; 6.
DR IntAct; Q14002; 2.
DR STRING; 9606.ENSP00000006724; -.
DR GlyGen; Q14002; 7 sites.
DR iPTMnet; Q14002; -.
DR PhosphoSitePlus; Q14002; -.
DR BioMuta; CEACAM7; -.
DR DMDM; 5921734; -.
DR jPOST; Q14002; -.
DR MassIVE; Q14002; -.
DR PaxDb; Q14002; -.
DR PeptideAtlas; Q14002; -.
DR PRIDE; Q14002; -.
DR ProteomicsDB; 59783; -. [Q14002-1]
DR ProteomicsDB; 59784; -. [Q14002-2]
DR Antibodypedia; 30805; 282 antibodies from 26 providers.
DR DNASU; 1087; -.
DR Ensembl; ENST00000006724.7; ENSP00000006724.3; ENSG00000007306.15. [Q14002-1]
DR Ensembl; ENST00000401731.6; ENSP00000385932.1; ENSG00000007306.15. [Q14002-1]
DR Ensembl; ENST00000602225.1; ENSP00000469597.1; ENSG00000007306.15. [Q14002-2]
DR GeneID; 1087; -.
DR KEGG; hsa:1087; -.
DR MANE-Select; ENST00000401731.6; ENSP00000385932.1; NM_001291485.2; NP_001278414.1.
DR UCSC; uc002ori.2; human. [Q14002-1]
DR CTD; 1087; -.
DR DisGeNET; 1087; -.
DR GeneCards; CEACAM7; -.
DR HGNC; HGNC:1819; CEACAM7.
DR HPA; ENSG00000007306; Tissue enriched (intestine).
DR MIM; 619160; gene.
DR neXtProt; NX_Q14002; -.
DR OpenTargets; ENSG00000007306; -.
DR PharmGKB; PA26363; -.
DR VEuPathDB; HostDB:ENSG00000007306; -.
DR eggNOG; ENOG502RXPD; Eukaryota.
DR GeneTree; ENSGT00960000186634; -.
DR HOGENOM; CLU_024555_4_0_1; -.
DR InParanoid; Q14002; -.
DR OrthoDB; 998214at2759; -.
DR PhylomeDB; Q14002; -.
DR TreeFam; TF336859; -.
DR PathwayCommons; Q14002; -.
DR Reactome; R-HSA-163125; Post-translational modification: synthesis of GPI-anchored proteins.
DR SignaLink; Q14002; -.
DR BioGRID-ORCS; 1087; 8 hits in 1058 CRISPR screens.
DR ChiTaRS; CEACAM7; human.
DR GeneWiki; CEACAM7; -.
DR GenomeRNAi; 1087; -.
DR Pharos; Q14002; Tbio.
DR PRO; PR:Q14002; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q14002; protein.
DR Bgee; ENSG00000007306; Expressed in mucosa of transverse colon and 108 other tissues.
DR ExpressionAtlas; Q14002; baseline and differential.
DR Genevisible; Q14002; HS.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016324; C:apical plasma membrane; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR Pfam; PF07686; V-set; 1.
DR SMART; SM00409; IG; 2.
DR SUPFAM; SSF48726; SSF48726; 2.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane;
KW Direct protein sequencing; Disulfide bond; Glycoprotein; GPI-anchor;
KW Immunoglobulin domain; Lipoprotein; Membrane; Reference proteome; Repeat;
KW Signal.
FT SIGNAL 1..35
FT /evidence="ECO:0000269|PubMed:15340161"
FT CHAIN 36..242
FT /note="Carcinoembryonic antigen-related cell adhesion
FT molecule 7"
FT /id="PRO_0000014570"
FT PROPEP 243..265
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000014571"
FT DOMAIN 36..142
FT /note="Ig-like V-type"
FT /evidence="ECO:0000250|UniProtKB:P31997"
FT DOMAIN 146..233
FT /note="Ig-like C2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT LIPID 242
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 57
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 105
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 112
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 174
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 198
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 168..216
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VAR_SEQ 143..235
FT /note="Missing (in isoform 2b)"
FT /evidence="ECO:0000305"
FT /id="VSP_002488"
FT VARIANT 120
FT /note="I -> F (in dbSNP:rs8102488)"
FT /evidence="ECO:0000269|PubMed:1427854,
FT ECO:0000269|PubMed:15057824, ECO:0000269|PubMed:7806520,
FT ECO:0000269|PubMed:9135022, ECO:0000269|Ref.3"
FT /id="VAR_024494"
FT VARIANT 236
FT /note="Y -> H (in dbSNP:rs16975478)"
FT /id="VAR_059384"
FT VARIANT 263
FT /note="A -> V (in dbSNP:rs7259532)"
FT /id="VAR_049848"
FT CONFLICT 40
FT /note="V -> G (in Ref. 3; AAB62924/AAB62925)"
FT /evidence="ECO:0000305"
FT CONFLICT 71
FT /note="E -> Q (in Ref. 3; AAB62924/AAB62925)"
FT /evidence="ECO:0000305"
FT CONFLICT 235
FT /note="R -> C (in Ref. 3; AAB62924)"
FT /evidence="ECO:0000305"
FT STRAND 37..46
FT /evidence="ECO:0007829|PDB:4Y89"
FT STRAND 51..56
FT /evidence="ECO:0007829|PDB:4Y89"
FT STRAND 62..71
FT /evidence="ECO:0007829|PDB:4Y89"
FT HELIX 75..77
FT /evidence="ECO:0007829|PDB:4Y89"
FT STRAND 78..83
FT /evidence="ECO:0007829|PDB:4Y89"
FT TURN 84..87
FT /evidence="ECO:0007829|PDB:4Y89"
FT STRAND 100..102
FT /evidence="ECO:0007829|PDB:4Y89"
FT STRAND 108..110
FT /evidence="ECO:0007829|PDB:4Y89"
FT HELIX 115..117
FT /evidence="ECO:0007829|PDB:4Y89"
FT STRAND 119..127
FT /evidence="ECO:0007829|PDB:4Y89"
FT STRAND 133..142
FT /evidence="ECO:0007829|PDB:4Y89"
SQ SEQUENCE 265 AA; 29345 MW; 16431DD2B0010D30 CRC64;
MGSPSACPYR VCIPWQGLLL TASLLTFWNL PNSAQTNIDV VPFNVAEGKE VLLVVHNESQ
NLYGYNWYKG ERVHANYRII GYVKNISQEN APGPAHNGRE TIYPNGTLLI QNVTHNDAGI
YTLHVIKENL VNEEVTRQFY VFSEPPKPSI TSNNFNPVEN KDIVVLTCQP ETQNTTYLWW
VNNQSLLVSP RLLLSTDNRT LVLLSATKND IGPYECEIQN PVGASRSDPV TLNVRYESVQ
ASSPDLSAGT AVSIMIGVLA GMALI
