ID   CEAM_ECOLX              Reviewed;         271 AA.
AC   P05820;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Colicin-M;
GN   Name=cma;
OS   Escherichia coli.
OG   Plasmid ColBM-Cl139.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3036784; DOI=10.1128/jb.169.7.3358-3361.1987;
RA   Koeck J., Oelschlaeger T., Kamp R.M., Braun V.;
RT   "Primary structure of colicin M, an inhibitor of murein biosynthesis.";
RL   J. Bacteriol. 169:3358-3361(1987).
CC   -!- FUNCTION: Colicins are polypeptide toxins produced by and active
CC       against E.coli and closely related bacteria.
CC   -!- FUNCTION: This is a calcium-requiring inhibitor for murein
CC       biosynthesis; it causes lysis of sensitive cells accompanied by murein
CC       degradation. The target site is possibly the cytoplasmic membrane.
CC   -!- MISCELLANEOUS: This colicin requires TonB for its uptake.
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DR   EMBL; M16754; AAA23589.1; -; Genomic_DNA.
DR   PIR; A27090; IKECM.
DR   RefSeq; WP_000449473.1; NZ_WTPM01000018.1.
DR   RefSeq; YP_008998009.1; NC_023315.1.
DR   PDB; 2XMX; X-ray; 1.67 A; A/B=1-271.
DR   PDB; 2XTQ; X-ray; 2.31 A; A/B/C/D/E/F/G/H=1-271.
DR   PDB; 2XTR; X-ray; 2.14 A; A/B=1-271.
DR   PDB; 3DA3; X-ray; 2.50 A; A/B=1-271.
DR   PDB; 3DA4; X-ray; 1.70 A; A/B=1-271.
DR   PDBsum; 2XMX; -.
DR   PDBsum; 2XTQ; -.
DR   PDBsum; 2XTR; -.
DR   PDBsum; 3DA3; -.
DR   PDBsum; 3DA4; -.
DR   AlphaFoldDB; P05820; -.
DR   SMR; P05820; -.
DR   IntAct; P05820; 1.
DR   EvolutionaryTrace; P05820; -.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR028056; Colicin_M.
DR   Pfam; PF14859; Colicin_M; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Bacteriocin; Plasmid; TonB box.
FT   CHAIN           1..271
FT                   /note="Colicin-M"
FT                   /id="PRO_0000218689"
FT   MOTIF           2..9
FT                   /note="TonB box"
FT   TURN            11..13
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   HELIX           18..21
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   HELIX           38..45
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   HELIX           49..65
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   STRAND          67..69
FT                   /evidence="ECO:0007829|PDB:2XTQ"
FT   HELIX           70..92
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   HELIX           98..104
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   HELIX           109..111
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   HELIX           114..117
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   STRAND          120..122
FT                   /evidence="ECO:0007829|PDB:3DA4"
FT   HELIX           128..138
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   STRAND          144..146
FT                   /evidence="ECO:0007829|PDB:3DA4"
FT   HELIX           148..150
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   HELIX           157..159
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   HELIX           161..168
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   STRAND          173..184
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   HELIX           185..187
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   HELIX           190..195
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   STRAND          198..209
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   STRAND          213..231
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   TURN            232..234
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   HELIX           237..249
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   STRAND          255..258
FT                   /evidence="ECO:0007829|PDB:2XMX"
FT   STRAND          263..270
FT                   /evidence="ECO:0007829|PDB:2XMX"
SQ   SEQUENCE   271 AA;  29483 MW;  B41B7BE107EC1DBA CRC64;
     METLTVHAPS PSTNLPSYGN GAFSLSAPHV PGAGPLLVQV VYSFFQSPNM CLQALTQLED
     YIKKHGASNP LTLQIISTNI GYFCNADRNL VLHPGISVYD AYHFAKPAPS QYDYRSMNMK
     QMSGNVTTPI VALAHYLWGN GAERSVNIAN IGLKISPMKI NQIKDIIKSG VVGTFPVSTK
     FTHATGDYNV ITGAYLGNIT LKTEGTLTIS ANGSWTYNGV VRSYDDKYDF NASTHRGIIG
     ESLTRLGAMF SGKEYQILLP GEIHIKESGK R