ID   CEAV_ECOLX              Reviewed;         103 AA.
AC   P22522;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Colicin-V;
DE   AltName: Full=Microcin-V bacteriocin;
DE   Flags: Precursor;
GN   Name=cvaC;
OS   Escherichia coli.
OG   Plasmid IncFI ColV3-K30.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2249654; DOI=10.1002/j.1460-2075.1990.tb07606.x;
RA   Gilson L., Mahanty H.K., Kolter R.;
RT   "Genetic analysis of an MDR-like export system: the secretion of colicin
RT   V.";
RL   EMBO J. 9:3875-3884(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Various strains;
RA   Pinou T., Riley M.A.;
RL   Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9743528; DOI=10.1084/jem.188.6.1091;
RA   Otto B.R., van Dooren S.J.M., Nuijens J.H., Luirink J., Oudega B.;
RT   "Characterization of a hemoglobin protease secreted by the pathogenic
RT   Escherichia coli strain EB1.";
RL   J. Exp. Med. 188:1091-1103(1998).
RN   [4]
RP   PROTEIN SEQUENCE OF 16-62, AND DISULFIDE BOND.
RX   PubMed=7952189; DOI=10.1099/13500872-140-9-2383;
RA   Havarstein L.S., Holo H., Nes I.F.;
RT   "The leader peptide of colicin V shares consensus sequences with leader
RT   peptides that are common among peptide bacteriocins produced by Gram-
RT   positive bacteria.";
RL   Microbiology 140:2383-2389(1994).
RN   [5]
RP   PROTEIN SEQUENCE OF 16-28, AND SUBCELLULAR LOCATION.
RX   PubMed=8204625; DOI=10.1021/bi00188a021;
RA   Fath M.J., Zhang L.H., Rush J., Kolter R.;
RT   "Purification and characterization of colicin V from Escherichia coli
RT   culture supernatants.";
RL   Biochemistry 33:6911-6917(1994).
CC   -!- FUNCTION: Colicin V kills sensitive cells by disrupting the membrane
CC       potential.
CC   -!- FUNCTION: Colicins are polypeptide toxins produced by, and active
CC       against E.coli and closely related bacteria.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:8204625}.
CC       Note=Secreted by the CvaAB/TolC export system.
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DR   EMBL; X57525; CAA40746.1; -; Genomic_DNA.
DR   EMBL; AF062844; AAC16351.1; -; Genomic_DNA.
DR   EMBL; AF062845; AAC16353.1; -; Genomic_DNA.
DR   EMBL; AF062846; AAC16355.1; -; Genomic_DNA.
DR   EMBL; AF062847; AAC16357.1; -; Genomic_DNA.
DR   EMBL; AF062848; AAC16359.1; -; Genomic_DNA.
DR   EMBL; AF062849; AAC16361.1; -; Genomic_DNA.
DR   EMBL; AF062850; AAC16363.1; -; Genomic_DNA.
DR   EMBL; AF062851; AAC16365.1; -; Genomic_DNA.
DR   EMBL; AF062852; AAC16367.1; -; Genomic_DNA.
DR   EMBL; AF062853; AAC16369.1; -; Genomic_DNA.
DR   EMBL; AF062854; AAC16371.1; -; Genomic_DNA.
DR   EMBL; AF062855; AAC16373.1; -; Genomic_DNA.
DR   EMBL; AF062856; AAC16375.1; -; Genomic_DNA.
DR   EMBL; AF062857; AAC16377.1; -; Genomic_DNA.
DR   EMBL; AF062858; AAC16379.1; -; Genomic_DNA.
DR   EMBL; AJ223631; CAA11514.1; -; Genomic_DNA.
DR   PIR; S12274; IKECV.
DR   RefSeq; WP_001259758.1; NZ_WVVL01000047.1.
DR   RefSeq; YP_002527505.1; NC_011964.1.
DR   RefSeq; YP_009070743.1; NC_025175.1.
DR   RefSeq; YP_444120.1; NC_007675.1.
DR   AlphaFoldDB; P22522; -.
DR   TCDB; 1.C.31.1.1; the channel-forming colicin v (colicin v) family.
DR   OMA; DKVHSWA; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR020280; MccV.
DR   Pfam; PF17508; MccV; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW   Disulfide bond; Plasmid; Secreted.
FT   PROPEP          1..15
FT                   /evidence="ECO:0000269|PubMed:7952189,
FT                   ECO:0000269|PubMed:8204625"
FT                   /id="PRO_0000005675"
FT   CHAIN           16..103
FT                   /note="Colicin-V"
FT                   /id="PRO_0000005676"
FT   DISULFID        91..102
FT                   /evidence="ECO:0000269|PubMed:7952189"
SQ   SEQUENCE   103 AA;  10311 MW;  E6BA4A98D2AAD649 CRC64;
     MRTLTLNELD SVSGGASGRD IAMAIGTLSG QFVAGGIGAA AGGVAGGAIY DYASTHKPNP
     AMSPSGLGGT IKQKPEGIPS EAWNYAAGRL CNWSPNNLSD VCL