CEBP2_CAEEL
ID CEBP2_CAEEL Reviewed; 100 AA.
AC Q8IG69;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=CCAAT/enhancer-binding protein homolog 2 {ECO:0000303|PubMed:26876169};
GN Name=cebp-2 {ECO:0000312|WormBase:C48E7.11};
GN ORFNames=C48E7.11 {ECO:0000312|WormBase:C48E7.11};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF ARG-39; MET-78; ALA-79
RP AND GLY-85.
RX PubMed=26505800; DOI=10.1016/j.bbrc.2015.10.106;
RA Xu X.Y., Hu J.P., Wu M.M., Wang L.S., Fang N.Y.;
RT "CCAAT/enhancer-binding protein CEBP-2 controls fat consumption and fatty
RT acid desaturation in Caenorhabditis elegans.";
RL Biochem. Biophys. Res. Commun. 468:312-318(2015).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=26876169; DOI=10.1016/j.celrep.2016.01.055;
RA Reddy K.C., Dunbar T.L., Nargund A.M., Haynes C.M., Troemel E.R.;
RT "The C. elegans CCAAT-Enhancer-Binding Protein Gamma Is Required for
RT Surveillance Immunity.";
RL Cell Rep. 14:1581-1589(2016).
RN [4] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=28662060; DOI=10.1371/journal.pgen.1006876;
RA Tjahjono E., Kirienko N.V.;
RT "A conserved mitochondrial surveillance pathway is required for defense
RT against Pseudomonas aeruginosa.";
RL PLoS Genet. 13:e1006876-e1006876(2017).
RN [5]
RP FUNCTION, INTERACTION WITH ZIP-11, AND DISRUPTION PHENOTYPE.
RX PubMed=34804026; DOI=10.3389/fimmu.2021.744454;
RA Zheng Z., Aihemaiti Y., Liu J., Afridi M.I., Yang S., Zhang X., Xu Y.,
RA Chen C., Tu H.;
RT "The bZIP Transcription Factor ZIP-11 Is Required for the Innate Immune
RT Regulation in Caenorhabditis elegans.";
RL Front. Immunol. 12:744454-744454(2021).
CC -!- FUNCTION: Transcription factor that binds to the promoter and the
CC enhancer regions of target genes (By similarity). Regulates expression
CC of genes involved in fat metabolism, including ech-1.1 and fat-5
CC (PubMed:26505800). Has a protective role in response to infection by
CC the Gram-negative bacterium P.aeruginosa (PubMed:26876169,
CC PubMed:28662060, PubMed:34804026). Required for the activation of
CC infection response gene irg-1 following P.aeruginosa infection
CC (PubMed:26876169). Required to prevent P.aeruginosa ToxA-mediated
CC lethality (PubMed:26876169). May also function in concert with
CC transcription factor zip-11 to mediate immune responses, independently
CC of the pmk-1/p38 MAPK pathway (PubMed:34804026). May act together with
CC the bZIP transcription factor, zip-2 (PubMed:26876169).
CC {ECO:0000250|UniProtKB:P53567, ECO:0000269|PubMed:26505800,
CC ECO:0000269|PubMed:26876169, ECO:0000269|PubMed:28662060,
CC ECO:0000269|PubMed:34804026}.
CC -!- SUBUNIT: Interacts with transcription factor zip-11.
CC {ECO:0000269|PubMed:34804026}.
CC -!- INTERACTION:
CC Q8IG69; Q21361: atf-2; NbExp=2; IntAct=EBI-2914231, EBI-317743;
CC Q8IG69; Q22156: atf-4; NbExp=3; IntAct=EBI-2914231, EBI-6749607;
CC Q8IG69; Q23272: atfs-1; NbExp=2; IntAct=EBI-2914231, EBI-6748337;
CC Q8IG69; Q94126: ces-2; NbExp=3; IntAct=EBI-2914231, EBI-328155;
CC Q8IG69; Q9N5A8: zip-11; NbExp=3; IntAct=EBI-2914231, EBI-6740132;
CC Q8IG69; Q21148: zip-2; NbExp=3; IntAct=EBI-2914231, EBI-6731556;
CC Q8IG69; Q9XUK2: zip-3; NbExp=3; IntAct=EBI-2914231, EBI-322774;
CC Q8IG69; A0A1I6CMA8: zip-9; NbExp=2; IntAct=EBI-2914231, EBI-26597024;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26876169}.
CC -!- TISSUE SPECIFICITY: Expressed broadly in somatic tissues including the
CC intestine. {ECO:0000269|PubMed:26876169}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown reduces overall fat
CC content (PubMed:26505800). Decreases survival upon infection with
CC P.aeruginosa (PubMed:26876169, PubMed:28662060). Reduces induction of
CC expression of infection response gene, irg-1, in response to
CC P.aeruginosa (PubMed:26876169). The decrease in survival upon
CC P.aeruginosa infection on a zip-11 mutant background is abolished by
CC RNAi-mediated knockdown of cebp-2. {ECO:0000269|PubMed:26505800,
CC ECO:0000269|PubMed:26876169, ECO:0000269|PubMed:28662060,
CC ECO:0000269|PubMed:34804026}.
CC -!- SIMILARITY: Belongs to the bZIP family. C/EBP subfamily. {ECO:0000305}.
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DR EMBL; BX284601; CCD64211.1; -; Genomic_DNA.
DR RefSeq; NP_871835.1; NM_182035.3.
DR AlphaFoldDB; Q8IG69; -.
DR SMR; Q8IG69; -.
DR IntAct; Q8IG69; 14.
DR STRING; 6239.C48E7.11; -.
DR EPD; Q8IG69; -.
DR PaxDb; Q8IG69; -.
DR PeptideAtlas; Q8IG69; -.
DR EnsemblMetazoa; C48E7.11.1; C48E7.11.1; WBGene00016754.
DR GeneID; 172319; -.
DR KEGG; cel:CELE_C48E7.11; -.
DR UCSC; C48E7.11; c. elegans.
DR CTD; 172319; -.
DR WormBase; C48E7.11; CE32167; WBGene00016754; cebp-2.
DR eggNOG; KOG3119; Eukaryota.
DR HOGENOM; CLU_2348671_0_0_1; -.
DR InParanoid; Q8IG69; -.
DR OMA; MAPKNKE; -.
DR OrthoDB; 1532157at2759; -.
DR PhylomeDB; Q8IG69; -.
DR SignaLink; Q8IG69; -.
DR PRO; PR:Q8IG69; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00016754; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0019216; P:regulation of lipid metabolic process; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:WormBase.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR Pfam; PF07716; bZIP_2; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
PE 1: Evidence at protein level;
KW Coiled coil; DNA-binding; Immunity; Innate immunity; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..100
FT /note="CCAAT/enhancer-binding protein homolog 2"
FT /id="PRO_0000451177"
FT DOMAIN 17..80
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 23..48
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 52..73
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 79..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 24..83
FT /evidence="ECO:0000255"
FT COMPBIAS 7..60
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 86..100
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 39
FT /note="R->C: In gk509377; low-fat phenotype. Increases
FT expression of ech-1.1 mRNA and decreases fat-5 mRNA."
FT /evidence="ECO:0000269|PubMed:26505800"
FT MUTAGEN 78
FT /note="M->I: In gk112657; low-fat phenotype."
FT /evidence="ECO:0000269|PubMed:26505800"
FT MUTAGEN 79
FT /note="A->T: In gk112658; low-fat phenotype."
FT /evidence="ECO:0000269|PubMed:26505800"
FT MUTAGEN 85
FT /note="G->E: In gk112659; low-fat phenotype."
FT /evidence="ECO:0000269|PubMed:26505800"
SQ SEQUENCE 100 AA; 11625 MW; C4A65961F3C71BC6 CRC64;
MSGNRKRNTS EPREDDEDDY STKRKRNNEA VNRTRQKKRQ EENDTAEKVD ELKKENETLE
RKVEQLQKEL SFLKEMFMAY AKNDGNDGPP PPPPPSSSAV
