CEBPZ_MOUSE
ID CEBPZ_MOUSE Reviewed; 1052 AA.
AC P53569; Q3TXW5; Q3TYA8; Q3UWQ7;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2006, sequence version 2.
DT 25-MAY-2022, entry version 156.
DE RecName: Full=CCAAT/enhancer-binding protein zeta;
DE AltName: Full=CCAAT-box-binding transcription factor;
DE Short=CBF;
DE Short=CCAAT-binding factor;
GN Name=Cebpz; Synonyms=Cbf2, Cebpa-rs1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS CBF1 AND CBF2).
RC STRAIN=BALB/cJ;
RX PubMed=8604343; DOI=10.1093/nar/24.6.1091;
RA Hoeppner M.A., Gilbert D.J., Copeland N.G., Jenkins N.A., Linzer D.I.H.,
RA Wu B.;
RT "Cloning and characterization of mouse CCAAT binding factor.";
RL Nucleic Acids Res. 24:1091-1098(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CBF1).
RC STRAIN=C57BL/6J, and CD-1; TISSUE=Egg, Rathke gland, and Visual cortex;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-641, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-629 AND SER-641, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Stimulates transcription from the HSP70 promoter.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=CBF1;
CC IsoId=P53569-1; Sequence=Displayed;
CC Name=CBF2;
CC IsoId=P53569-2; Sequence=VSP_000853, VSP_000854;
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- SIMILARITY: Belongs to the CBF/MAK21 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB01503.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAE34799.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U19891; AAB01503.1; ALT_FRAME; mRNA.
DR EMBL; U19892; AAB01504.1; -; mRNA.
DR EMBL; AK136173; BAE22857.1; -; mRNA.
DR EMBL; AK158771; BAE34655.1; -; mRNA.
DR EMBL; AK159081; BAE34799.1; ALT_FRAME; mRNA.
DR CCDS; CCDS28981.1; -. [P53569-1]
DR PIR; JC6067; JC6067.
DR RefSeq; NP_001019977.1; NM_001024806.2.
DR AlphaFoldDB; P53569; -.
DR BioGRID; 198668; 41.
DR CORUM; P53569; -.
DR IntAct; P53569; 1.
DR STRING; 10090.ENSMUSP00000024885; -.
DR iPTMnet; P53569; -.
DR PhosphoSitePlus; P53569; -.
DR EPD; P53569; -.
DR jPOST; P53569; -.
DR MaxQB; P53569; -.
DR PaxDb; P53569; -.
DR PeptideAtlas; P53569; -.
DR PRIDE; P53569; -.
DR ProteomicsDB; 281527; -. [P53569-1]
DR ProteomicsDB; 281528; -. [P53569-2]
DR DNASU; 12607; -.
DR GeneID; 12607; -.
DR KEGG; mmu:12607; -.
DR UCSC; uc008dpk.1; mouse. [P53569-1]
DR CTD; 10153; -.
DR MGI; MGI:109386; Cebpz.
DR eggNOG; KOG2038; Eukaryota.
DR InParanoid; P53569; -.
DR OrthoDB; 551907at2759; -.
DR PhylomeDB; P53569; -.
DR TreeFam; TF105010; -.
DR BioGRID-ORCS; 12607; 24 hits in 73 CRISPR screens.
DR ChiTaRS; Cebpz; mouse.
DR PRO; PR:P53569; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P53569; protein.
DR GO; GO:0016602; C:CCAAT-binding factor complex; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0003713; F:transcription coactivator activity; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR005612; CCAAT-binding_factor.
DR InterPro; IPR040155; CEBPZ/Mak21-like.
DR PANTHER; PTHR12048; PTHR12048; 1.
DR Pfam; PF03914; CBF; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..1052
FT /note="CCAAT/enhancer-binding protein zeta"
FT /id="PRO_0000173471"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 122..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 621..677
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 876..969
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1032..1052
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 122..146
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 629..643
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 644..677
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 887..931
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 939..969
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 629
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 641
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 837
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q03701"
FT MOD_RES 958
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q03701"
FT MOD_RES 972
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q03701"
FT MOD_RES 977
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q03701"
FT VAR_SEQ 455..461
FT /note="SELANKL -> KRCWIQA (in isoform CBF2)"
FT /evidence="ECO:0000303|PubMed:8604343"
FT /id="VSP_000853"
FT VAR_SEQ 462..1052
FT /note="Missing (in isoform CBF2)"
FT /evidence="ECO:0000303|PubMed:8604343"
FT /id="VSP_000854"
FT CONFLICT 112
FT /note="A -> T (in Ref. 2; BAE22857/BAE34655)"
FT /evidence="ECO:0000305"
FT CONFLICT 146
FT /note="A -> P (in Ref. 2; BAE22857/BAE34655)"
FT /evidence="ECO:0000305"
FT CONFLICT 196
FT /note="P -> L (in Ref. 2; BAE22857/BAE34655)"
FT /evidence="ECO:0000305"
FT CONFLICT 318
FT /note="M -> L (in Ref. 2; BAE22857/BAE34655)"
FT /evidence="ECO:0000305"
FT CONFLICT 422
FT /note="G -> A (in Ref. 2; BAE22857/BAE34655)"
FT /evidence="ECO:0000305"
FT CONFLICT 471
FT /note="T -> S (in Ref. 1; AAB01503)"
FT /evidence="ECO:0000305"
FT CONFLICT 652
FT /note="G -> E (in Ref. 2; BAE22857/BAE34655)"
FT /evidence="ECO:0000305"
FT CONFLICT 875
FT /note="T -> A (in Ref. 2; BAE22857/BAE34655)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1052 AA; 120262 MW; 8518FBAA30D9C41C CRC64;
MSADQEPVAF LAKQPWRPKQ VTEDPDEEDE EDGDEGKNGF SLEEVLRLGG TKQDYLMLAT
LDENEEVVDG GKKGTIDDLQ QGELESFIQN LNLAKYSKSL IEEDEPEKKE NASKKEAKLL
KVENKKQKAT EGKKTSEKKV KNKTVAEQRP ESCPVSKAKK DKQPDVFEFL ERQTMLLRPG
GKWYDMEYSG EYSLEPQPPD VVSKYKALAQ KLYEHEVSLF KSKTNNQKGG SSTWMKAIVS
SGTLADRMAA MILLIQDDAV HTLQFVETLM SLVKKKGSKQ QCLMALDTFK ELLITDLLPD
SRKLRVFSQH PFHKLEEMSS GNKDSRDRRL ILWYYEHQLK HLVAEFVQVL ETLSHDSLVT
TKTRALVAAH ELLCDKPEEE KALLVQVINK LGDPQNRIAT KASHLLEVLL RKHPNMKGVV
CGEIERLLFR SNISPKAQYY AICFLNQMVL SHEESELANK LITLYFCFFR TCIKKKDIES
KMLSAILTGV NRAYPYSQIG DDKVREQVDT LFKVLHVVNF NTSVQALMLL FQVMNSQQTI
SDRYYTALYR KMLDPGLTTC SKQAMFLNLI YKSLKADIML RRVKAFVKRL LQVTCTQMPP
FICGALYLVS EILKAKPDLR SQLDDHPESD EENFVDVGDD SDDEKFTDAD KGTATDAVKE
VESKETEPES SAEAEKPKAA SWVHFDNLKG GKQIKTYDPF SRNPLFCGAE NTTLWELKKL
SEHFHPSVAL FAKTILEGNC IQYSGDPLQD FTLMRFLDRF VYRNPKLHKG KENTDSVVMQ
PKRKHFMKNV RDLAVNSKEF LAKEESQIPV DEVFFYRYYK KVAVVKDKQK RSADEESIED
IDDEEFENMI DTFEDDNCFP PGKDDIDFAS NMKKTKGAKA DLEDSESSDG ELGDLDDDEV
SLGSMNDEDF EIDEDGGTFM DVSDDESEDA PEFADANPKA NTKKSKRKSE DDFDFAGSFQ
GQKKKKKSFN DSSLFVSAEE FGHLLDENMG SKFDTIGMNA MANRDNASFK QLKWEAERDD
WLHNRDVKSI IKKKKNFRKK MKAPQKPKRQ RK
