CECA_AEDAE
ID CECA_AEDAE Reviewed; 59 AA.
AC P82592; Q17NR3; Q95PI9;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2008, sequence version 2.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Cecropin-A;
DE Flags: Precursor;
GN Name=CECA; ORFNames=AAEL000627;
OS Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Aedini; Aedes; Stegomyia.
OX NCBI_TaxID=7159;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-55, FUNCTION,
RP DEVELOPMENTAL STAGE, MASS SPECTROMETRY, AND VARIANT ARG-58.
RC STRAIN=Liverpool; TISSUE=Hemolymph;
RX PubMed=10400619; DOI=10.1074/jbc.274.29.20092;
RA Lowenberger C.A., Charlet M., Vizioli J., Kamal S., Richman A.,
RA Christensen B.M., Bulet P.;
RT "Antimicrobial activity spectrum, cDNA cloning and mRNA expression of a
RT newly isolated member of the cecropin family from the mosquito vector Aedes
RT aegypti.";
RL J. Biol. Chem. 274:20092-20097(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT ARG-58.
RC STRAIN=Moyo-R, and Red-eye;
RA Morlais I., Severson D.W.;
RT "Single nucleotide polymorphism and codon usage bias in Aedes aegypti.";
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Moyo-R;
RA Morlais I., Severson D.W.;
RT "Aedes aegypti cecropin A gene.";
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LVPib12;
RX PubMed=17510324; DOI=10.1126/science.1138878;
RA Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL Science 316:1718-1723(2007).
CC -!- FUNCTION: Antibacterial activity against Gram-negative bacteria E.coli
CC D22 and D31, E.carotovora, K.pneumoniae, P.aeruginosa, S.typhimurium,
CC E.cloacae B12 and X.campestris and Gram-positive bacteria A.viridans,
CC M.luteus, B.megaterium and S.pyogenes. Possesses antifungal activity
CC against F.oxysporum, F.culmorum and N.crassa, C.albicans, C.neoformans
CC and S.cerevisiae. No activity against Gram-negative S.marcescens Db11,
CC Gram-positive B.cereus, B.subtilis, B.thuringiensis, S.aureus and
CC L.monocytogenes, the fungi A.fumigatus and B.bassiana and C.glabrata.
CC {ECO:0000269|PubMed:10400619}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- DEVELOPMENTAL STAGE: Expressed in adults within 6 hours after induction
CC and continued to accumulate over 7-10 days.
CC {ECO:0000269|PubMed:10400619}.
CC -!- INDUCTION: By bacterial infection.
CC -!- MASS SPECTROMETRY: Mass=3391.5; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10400619};
CC -!- SIMILARITY: Belongs to the cecropin family. {ECO:0000305}.
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DR EMBL; AF117886; AAF59831.1; -; mRNA.
DR EMBL; AY064080; AAL85581.1; -; Genomic_DNA.
DR EMBL; AY064081; AAL85582.1; -; Genomic_DNA.
DR EMBL; AF387488; AAK73081.1; -; mRNA.
DR EMBL; CH477197; EAT48340.1; -; Genomic_DNA.
DR RefSeq; XP_001649179.1; XM_001649129.2.
DR AlphaFoldDB; P82592; -.
DR SMR; P82592; -.
DR eggNOG; ENOG502T8YY; Eukaryota.
DR HOGENOM; CLU_187909_1_1_1; -.
DR InParanoid; P82592; -.
DR Proteomes; UP000008820; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019731; P:antibacterial humoral response; IEA:InterPro.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IEA:UniProt.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IEA:UniProt.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR InterPro; IPR000875; Cecropin.
DR InterPro; IPR020400; Cecropin_insect.
DR PANTHER; PTHR38329; PTHR38329; 1.
DR Pfam; PF00272; Cecropin; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Direct protein sequencing; Fungicide; Immunity;
KW Innate immunity; Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:10400619"
FT CHAIN 24..57
FT /note="Cecropin-A"
FT /id="PRO_0000004810"
FT PROPEP 58..59
FT /note="Removed in mature form"
FT /id="PRO_0000004811"
FT VARIANT 58
FT /note="G -> R (in strain: Red-eye)"
FT /evidence="ECO:0000269|PubMed:10400619, ECO:0000269|Ref.2"
FT CONFLICT 12
FT /note="M -> V (in Ref. 1; AAF59831, 2; AAL85581/AAL85582
FT and 3; AAK73081)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 59 AA; 6083 MW; EAB5234635071639 CRC64;
MNFTKLFLLI AMAVLLLTGQ SEAGGLKKLG KKLEGAGKRV FNAAEKALPV VAGAKALGK
