CECA_ANOGA
ID CECA_ANOGA Reviewed; 58 AA.
AC P82290; A7XAX5; A7XAX7; A7XAY1; A7XAY8; A7XAZ7; A7XB12; A7XB23; Q7QEE3;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Cecropin-A;
DE Contains:
DE RecName: Full=Cecropin-A;
DE Contains:
DE RecName: Full=Cecropin-A amidated isoform;
DE Flags: Precursor;
GN Name=CecA; Synonyms=CEC1; ORFNames=AGAP000693;
OS Anopheles gambiae (African malaria mosquito).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Anophelinae; Anopheles.
OX NCBI_TaxID=7165;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-33, FUNCTION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, MASS SPECTROMETRY, AND AMIDATION AT
RP LEU-57.
RC STRAIN=G3;
RX PubMed=10672074; DOI=10.1046/j.1365-2583.2000.00164.x;
RA Vizioli J., Bulet P., Charlet M., Lowenberger C., Blass C., Mueller H.-M.,
RA Dimopoulos G.M., Hoffmann J., Kafatos F.C., Richman A.;
RT "Cloning and analysis of a cecropin gene from the malaria vector mosquito,
RT Anopheles gambiae.";
RL Insect Mol. Biol. 9:75-84(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12421409; DOI=10.1046/j.1365-2583.2002.00360.x;
RA Zheng X.-L., Zheng A.L.;
RT "Genomic organization and regulation of three cecropin genes in Anopheles
RT gambiae.";
RL Insect Mol. Biol. 11:517-525(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS HIS-2; PHE-8; VAL-16;
RP HIS-20 AND ASP-34.
RC STRAIN=GAM12A, GAM12B, GAM2A, GAM2B, GAM3A, GAM3B, GAM4A, GAM4B, GAM5,
RC GAM57B, GAM59A, GAM5A, GAM60A, GAM61, GAM67B, GAM6A, GAM6B, GAM70A, GAM70B,
RC GAM73A, GAM74B, and GAM8B;
RX PubMed=17726523; DOI=10.1371/journal.pone.0000793;
RA Slotman M.A., Parmakelis A., Marshall J.C., Awono-Ambene P.H.,
RA Antonio-Nkondjo C., Simard F., Caccone A., Powell J.R.;
RT "Patterns of selection in anti-malarial immune genes in malaria vectors:
RT evidence for adaptive evolution in LRIM1 in Anopheles arabiensis.";
RL PLoS ONE 2:E793-E793(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PEST;
RX PubMed=12364791; DOI=10.1126/science.1076181;
RA Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R.,
RA Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R.,
RA Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z.,
RA Kraft C.L., Abril J.F., Anthouard V., Arensburger P., Atkinson P.W.,
RA Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C.,
RA Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K.,
RA Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V.,
RA Dana A., Delcher A., Dew I., Evans C.A., Flanigan M.,
RA Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R.,
RA Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J.,
RA Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I.,
RA Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A.,
RA McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D.,
RA O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H.,
RA Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J.,
RA Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B.,
RA Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M.,
RA Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I.,
RA Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J.,
RA Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M.,
RA Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C.,
RA Collins F.H., Hoffman S.L.;
RT "The genome sequence of the malaria mosquito Anopheles gambiae.";
RL Science 298:129-149(2002).
CC -!- FUNCTION: Antibacterial activity against several Gram-positive and
CC Gram-negative bacteria. Antifungal activity against A.fumigatus,
CC B.cinerea, F.culmorum, F.oxysporum, N.crassa, C.albicans, C.neoformans
CC and S.cerevisiae. {ECO:0000269|PubMed:10672074}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Relatively abundant in head, thorax and to a lesser
CC extent in abdominal carcass and anterior midgut.
CC {ECO:0000269|PubMed:10672074}.
CC -!- DEVELOPMENTAL STAGE: In young pupae, expressed within 2 hours of
CC infection and continued to accumulate over 16 hours.
CC {ECO:0000269|PubMed:10672074}.
CC -!- INDUCTION: By bacterial infection.
CC -!- MASS SPECTROMETRY: [Cecropin-A]: Mass=3588.1; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10672074};
CC -!- MASS SPECTROMETRY: [Cecropin-A amidated isoform]: Mass=3530.2;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:10672074};
CC -!- SIMILARITY: Belongs to the cecropin family. {ECO:0000305}.
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DR EMBL; AF200686; AAF22649.1; -; mRNA.
DR EMBL; AF525673; AAM82610.1; -; Genomic_DNA.
DR EMBL; EU073490; ABU80069.1; -; Genomic_DNA.
DR EMBL; EU073491; ABU80070.1; -; Genomic_DNA.
DR EMBL; EU073492; ABU80071.1; -; Genomic_DNA.
DR EMBL; EU073493; ABU80072.1; -; Genomic_DNA.
DR EMBL; EU073494; ABU80073.1; -; Genomic_DNA.
DR EMBL; EU073495; ABU80074.1; -; Genomic_DNA.
DR EMBL; EU073496; ABU80075.1; -; Genomic_DNA.
DR EMBL; EU073497; ABU80076.1; -; Genomic_DNA.
DR EMBL; EU073498; ABU80077.1; -; Genomic_DNA.
DR EMBL; EU073499; ABU80078.1; -; Genomic_DNA.
DR EMBL; EU073500; ABU80079.1; -; Genomic_DNA.
DR EMBL; EU073501; ABU80080.1; -; Genomic_DNA.
DR EMBL; EU073502; ABU80081.1; -; Genomic_DNA.
DR EMBL; EU073503; ABU80082.1; -; Genomic_DNA.
DR EMBL; EU073504; ABU80083.1; -; Genomic_DNA.
DR EMBL; EU073505; ABU80084.1; -; Genomic_DNA.
DR EMBL; EU073506; ABU80085.1; -; Genomic_DNA.
DR EMBL; EU073507; ABU80086.1; -; Genomic_DNA.
DR EMBL; EU073508; ABU80087.1; -; Genomic_DNA.
DR EMBL; EU073509; ABU80088.1; -; Genomic_DNA.
DR EMBL; EU073510; ABU80089.1; -; Genomic_DNA.
DR EMBL; EU073511; ABU80090.1; -; Genomic_DNA.
DR EMBL; AAAB01008847; EAA06858.2; -; Genomic_DNA.
DR AlphaFoldDB; P82290; -.
DR SMR; P82290; -.
DR STRING; 7165.AGAP000693-PA; -.
DR PaxDb; P82290; -.
DR VEuPathDB; VectorBase:AGAP000693; -.
DR eggNOG; ENOG502T8YY; Eukaryota.
DR HOGENOM; CLU_187909_1_1_1; -.
DR InParanoid; P82290; -.
DR OrthoDB; 1632391at2759; -.
DR PhylomeDB; P82290; -.
DR Proteomes; UP000007062; Chromosome X.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IBA:GO_Central.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR InterPro; IPR000875; Cecropin.
DR InterPro; IPR020400; Cecropin_insect.
DR PANTHER; PTHR38329; PTHR38329; 1.
DR Pfam; PF00272; Cecropin; 1.
PE 1: Evidence at protein level;
KW Amidation; Antibiotic; Antimicrobial; Direct protein sequencing; Fungicide;
KW Immunity; Innate immunity; Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:10672074"
FT CHAIN 24..58
FT /note="Cecropin-A"
FT /id="PRO_0000004820"
FT CHAIN 24..57
FT /note="Cecropin-A amidated isoform"
FT /id="PRO_0000004821"
FT MOD_RES 57
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:10672074"
FT VARIANT 2
FT /note="N -> H (in strain: GAM4B and GAM6B)"
FT /evidence="ECO:0000269|PubMed:17726523"
FT VARIANT 8
FT /note="I -> F (in strain: GAM4B)"
FT /evidence="ECO:0000269|PubMed:17726523"
FT VARIANT 16
FT /note="L -> V (in strain: GAM5A and GAM5B)"
FT /evidence="ECO:0000269|PubMed:17726523"
FT VARIANT 20
FT /note="Q -> H (in strain: GAM2B and GAM8B)"
FT /evidence="ECO:0000269|PubMed:17726523"
FT VARIANT 34
FT /note="E -> D (in strain: GAM12B)"
FT /evidence="ECO:0000269|PubMed:17726523"
SQ SEQUENCE 58 AA; 6159 MW; DEB1FBC1B3626A7A CRC64;
MNFSKIFIFV VLAVLLLCSQ TEAGRLKKLG KKIEGAGKRV FKAAEKALPV VAGVKALG
