CECB_DROME
ID CECB_DROME Reviewed; 63 AA.
AC P14956; O16826; O16827; O16828; O18647; O61274; Q9V425;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Cecropin-B;
DE Flags: Precursor;
GN Name=CecB; ORFNames=CG1878;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Canton-S;
RX PubMed=2104802; DOI=10.1002/j.1460-2075.1990.tb08098.x;
RA Kylsten P., Samakovlis C., Hultmark D.;
RT "The cecropin locus in Drosophila; a compact gene cluster involved in the
RT response to infection.";
RL EMBO J. 9:217-224(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS LEU-10; ILE-12; VAL-43 AND
RP GLY-59.
RC STRAIN=B115, B137, B141, B202, B208, B222, B225, B226, Z10, Z18, Z22, and
RC Z24;
RX PubMed=9335607; DOI=10.1093/genetics/147.2.713;
RA Clark A.G., Wang L.;
RT "Molecular population genetics of Drosophila immune system genes.";
RL Genetics 147:713-724(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT LEU-10.
RC STRAIN=M11, M2, M26, M40, M47, M54, M55, and M66;
RX PubMed=9725836; DOI=10.1093/genetics/150.1.157;
RA Ramos-Onsins S., Aguade M.;
RT "Molecular evolution of the Cecropin multigene family in Drosophila:
RT functional genes vs pseudogenes.";
RL Genetics 150:157-171(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS LEU-10 AND VAL-54.
RC STRAIN=MA1, MA2, MA3, MJ1, MJ2, and MJ3;
RX PubMed=9553148; DOI=10.1007/s002510050379;
RA Date A., Satta Y., Takahata N., Chigusa S.I.;
RT "Evolutionary history and mechanism of the Drosophila cecropin gene
RT family.";
RL Immunogenetics 47:417-429(1998).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [7]
RP INDUCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S;
RX PubMed=2390977; DOI=10.1002/j.1460-2075.1990.tb07489.x;
RA Samakovlis C., Kimbrell D.A., Kylsten P., Engstrom A., Hultmark D.;
RT "The immune response in Drosophila: pattern of cecropin expression and
RT biological activity.";
RL EMBO J. 9:2969-2976(1990).
CC -!- FUNCTION: Cecropins have lytic and antibacterial activity against
CC several Gram-positive and Gram-negative bacteria.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Strongly expressed in larval, pupal and adult fat
CC body and hemocytes after injection of bacteria. Maximal expression is
CC seen in pupae. {ECO:0000269|PubMed:2390977}.
CC -!- INDUCTION: Induced as part of the humoral response to a bacterial
CC invasion. Transcripts appear within one hour after injection of
CC bacteria into the hemocoel, reach a maximum after 2-6 hours and have
CC almost disappeared after 24 hours. Similar response is seen when flies
CC ingest bacteria present in their food. {ECO:0000269|PubMed:2390977}.
CC -!- SIMILARITY: Belongs to the cecropin family. {ECO:0000305}.
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DR EMBL; X16972; CAA34845.1; -; Genomic_DNA.
DR EMBL; AF018994; AAB82491.1; -; Genomic_DNA.
DR EMBL; AF018995; AAB82492.1; -; Genomic_DNA.
DR EMBL; AF018996; AAB82493.1; -; Genomic_DNA.
DR EMBL; AF018997; AAB82494.1; -; Genomic_DNA.
DR EMBL; AF018998; AAB82495.1; -; Genomic_DNA.
DR EMBL; AF018999; AAB82496.1; -; Genomic_DNA.
DR EMBL; AF019000; AAB82497.1; -; Genomic_DNA.
DR EMBL; AF019001; AAB82498.1; -; Genomic_DNA.
DR EMBL; AF019002; AAB82499.1; -; Genomic_DNA.
DR EMBL; AF019003; AAB82500.1; -; Genomic_DNA.
DR EMBL; AF019004; AAB82501.1; -; Genomic_DNA.
DR EMBL; AF019005; AAB82502.1; -; Genomic_DNA.
DR EMBL; Y16852; CAA76429.1; -; Genomic_DNA.
DR EMBL; Y16853; CAA76435.1; -; Genomic_DNA.
DR EMBL; Y16854; CAA76441.1; -; Genomic_DNA.
DR EMBL; Y16855; CAA76447.1; -; Genomic_DNA.
DR EMBL; Y16856; CAA76453.1; -; Genomic_DNA.
DR EMBL; Y16858; CAA76465.1; -; Genomic_DNA.
DR EMBL; Y16859; CAA76471.1; -; Genomic_DNA.
DR EMBL; Y16861; CAA76481.1; -; Genomic_DNA.
DR EMBL; AB010791; BAA28722.1; -; Genomic_DNA.
DR EMBL; AB010792; BAA28726.1; -; Genomic_DNA.
DR EMBL; AB010793; BAA28729.1; -; Genomic_DNA.
DR EMBL; AB010794; BAA28733.1; -; Genomic_DNA.
DR EMBL; AB010795; BAA28735.1; -; Genomic_DNA.
DR EMBL; AB010796; BAA28739.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF57027.1; -; Genomic_DNA.
DR PIR; S07666; S07666.
DR RefSeq; NP_524590.1; NM_079851.3.
DR AlphaFoldDB; P14956; -.
DR DIP; DIP-22949N; -.
DR STRING; 7227.FBpp0085002; -.
DR PaxDb; P14956; -.
DR EnsemblMetazoa; FBtr0085640; FBpp0085002; FBgn0000278.
DR GeneID; 43598; -.
DR KEGG; dme:Dmel_CG1878; -.
DR CTD; 43598; -.
DR FlyBase; FBgn0000278; CecB.
DR VEuPathDB; VectorBase:FBgn0000278; -.
DR eggNOG; ENOG502TCNK; Eukaryota.
DR GeneTree; ENSGT00540000073806; -.
DR HOGENOM; CLU_187909_1_0_1; -.
DR InParanoid; P14956; -.
DR OMA; GHSEANW; -.
DR OrthoDB; 1632391at2759; -.
DR PhylomeDB; P14956; -.
DR BioGRID-ORCS; 43598; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43598; -.
DR PRO; PR:P14956; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0000278; Expressed in seminal fluid secreting gland and 13 other tissues.
DR ExpressionAtlas; P14956; baseline and differential.
DR Genevisible; P14956; DM.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0019731; P:antibacterial humoral response; IDA:FlyBase.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR GO; GO:0002213; P:defense response to insect; IEP:FlyBase.
DR GO; GO:0006959; P:humoral immune response; IEP:FlyBase.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR InterPro; IPR000875; Cecropin.
DR InterPro; IPR020400; Cecropin_insect.
DR PANTHER; PTHR38329; PTHR38329; 1.
DR Pfam; PF00272; Cecropin; 1.
DR PROSITE; PS00268; CECROPIN; 1.
PE 2: Evidence at transcript level;
KW Amidation; Antibiotic; Antimicrobial; Immunity; Innate immunity;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT CHAIN 24..62
FT /note="Cecropin-B"
FT /id="PRO_0000004843"
FT MOD_RES 62
FT /note="Arginine amide"
FT /evidence="ECO:0000250"
FT VARIANT 10
FT /note="V -> L (in strain: B141, M2, M40 and MJ2)"
FT /evidence="ECO:0000269|PubMed:9335607,
FT ECO:0000269|PubMed:9553148, ECO:0000269|PubMed:9725836"
FT VARIANT 12
FT /note="L -> I (in strain: B208)"
FT /evidence="ECO:0000269|PubMed:9335607"
FT VARIANT 43
FT /note="A -> V (in strain: Z24)"
FT /evidence="ECO:0000269|PubMed:9335607"
FT VARIANT 54
FT /note="A -> V (in strain: MA1)"
FT /evidence="ECO:0000269|PubMed:9553148"
FT VARIANT 59
FT /note="A -> G (in strain: B222, B226, Z10, Z22, Z18 and
FT Z24)"
FT /evidence="ECO:0000269|PubMed:9335607"
SQ SEQUENCE 63 AA; 6778 MW; 26D0251BF3C28AB4 CRC64;
MNFNKIFVFV ALILAISLGN SEAGWLRKLG KKIERIGQHT RDASIQVLGI AQQAANVAAT
ARG
