CECR_ECOLI
ID CECR_ECOLI Reviewed; 223 AA.
AC P0ACU0; P41037; P78054;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=HTH-type transcriptional dual regulator CecR {ECO:0000305};
DE AltName: Full=Regulator of cefoperazone and chloramphenicol sensitivity {ECO:0000303|PubMed:27112147};
GN Name=cecR {ECO:0000303|PubMed:27112147}; Synonyms=ybiH;
GN OrderedLocusNames=b0796, JW0780;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-103.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8037924; DOI=10.1266/jjg.69.1;
RA Ohmori H.;
RT "Structural analysis of the rhlE gene of Escherichia coli.";
RL Jpn. J. Genet. 69:1-12(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION, DNA-BINDING, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=27112147; DOI=10.1099/mic.0.000292;
RA Yamanaka Y., Shimada T., Yamamoto K., Ishihama A.;
RT "Transcription factor CecR (YbiH) regulates a set of genes affecting the
RT sensitivity of Escherichia coli against cefoperazone and chloramphenicol.";
RL Microbiology 162:1253-1264(2016).
CC -!- FUNCTION: Regulates transcription of the cecR-ybhGFSR operon and the
CC rhlE gene, which altogether are involved in the control of sensitivity
CC to cefoperazone and chloramphenicol. Represses the cecR-ybhGFSR operon
CC and activates the rhlE operon. Acts by binding to a palindromic
CC sequence within the intergenic spacer located between these two
CC divergently transcribed operons. {ECO:0000269|PubMed:27112147}.
CC -!- INTERACTION:
CC P0ACU0; P0ACU0: cecR; NbExp=2; IntAct=EBI-1114129, EBI-1114129;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Negatively autoregulated. {ECO:0000269|PubMed:27112147}.
CC -!- DISRUPTION PHENOTYPE: Mutant shows increased sensitivity to
CC chloramphenicol and cefoperazone, a third-generation cephalosporin.
CC {ECO:0000269|PubMed:27112147}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L02123; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U00096; AAC73883.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA35456.1; -; Genomic_DNA.
DR RefSeq; NP_415317.4; NC_000913.3.
DR RefSeq; WP_001296991.1; NZ_SSZK01000002.1.
DR AlphaFoldDB; P0ACU0; -.
DR SMR; P0ACU0; -.
DR BioGRID; 4259961; 7.
DR BioGRID; 849795; 3.
DR IntAct; P0ACU0; 10.
DR STRING; 511145.b0796; -.
DR jPOST; P0ACU0; -.
DR PaxDb; P0ACU0; -.
DR PRIDE; P0ACU0; -.
DR EnsemblBacteria; AAC73883; AAC73883; b0796.
DR EnsemblBacteria; BAA35456; BAA35456; BAA35456.
DR GeneID; 58389455; -.
DR GeneID; 945421; -.
DR KEGG; ecj:JW0780; -.
DR KEGG; eco:b0796; -.
DR PATRIC; fig|1411691.4.peg.1482; -.
DR EchoBASE; EB2306; -.
DR eggNOG; COG1309; Bacteria.
DR HOGENOM; CLU_069356_16_0_6; -.
DR InParanoid; P0ACU0; -.
DR OMA; EVLAFRM; -.
DR PhylomeDB; P0ACU0; -.
DR BioCyc; EcoCyc:EG12406-MON; -.
DR PRO; PR:P0ACU0; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:2000143; P:negative regulation of DNA-templated transcription, initiation; IDA:EcoCyc.
DR GO; GO:2000144; P:positive regulation of DNA-templated transcription, initiation; IDA:EcoCyc.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR023772; DNA-bd_HTH_TetR-type_CS.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001647; HTH_TetR.
DR InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR InterPro; IPR015292; Tscrpt_reg_YbiH_C.
DR Pfam; PF09209; CecR_C; 1.
DR Pfam; PF00440; TetR_N; 1.
DR PRINTS; PR00455; HTHTETR.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF48498; SSF48498; 1.
DR PROSITE; PS01081; HTH_TETR_1; 1.
DR PROSITE; PS50977; HTH_TETR_2; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; DNA-binding; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..223
FT /note="HTH-type transcriptional dual regulator CecR"
FT /id="PRO_0000070632"
FT DOMAIN 11..70
FT /note="HTH tetR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT DNA_BIND 33..52
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
SQ SEQUENCE 223 AA; 25017 MW; B85EBC247EE8DA86 CRC64;
MNNPAMTIKG EQAKKQLIAA ALAQFGEYGM NATTREIAAQ AGQNIAAITY YFGSKEDLYL
ACAQWIADFI GEQFRPHAEE AERLFAQPQP DRAAIRELIL RACRNMIKLL TQDDTVNLSK
FISREQLSPT AAYHLVHEQV ISPLHSHLTR LIAAWTGCDA NDTRMILHTH ALIGEILAFR
LGKETILLRT GWTAFDEEKT ELINQTVTCH IDLILQGLSQ RSL
