ID   CED12_CAEBR             Reviewed;         740 AA.
AC   A8XEZ1;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-MAY-2016, sequence version 4.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Cell death abnormality protein 12 {ECO:0000250|UniProtKB:Q8STE5};
GN   Name=ced-12 {ECO:0000312|WormBase:CBG12216};
GN   ORFNames=CBG12216 {ECO:0000312|WormBase:CBG12216};
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- FUNCTION: Involved in apoptosis and necrosis. Required for the cell
CC       corpse engulfment process. Has roles in the formation of actin halos
CC       and distal tip cell migration. Plays no role in amphid axon outgrowth
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with psr-1. Forms a ternary complex with ced-2 and
CC       ced-5 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Note=Punctate
CC       localization. {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAP31213.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAP31213.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; HE600958; CAP31213.2; ALT_SEQ; Genomic_DNA.
DR   AlphaFoldDB; A8XEZ1; -.
DR   SMR; A8XEZ1; -.
DR   STRING; 6238.CBG12216; -.
DR   EnsemblMetazoa; CBG12216.1; CBG12216.1; WBGene00033199.
DR   WormBase; CBG12216; CBP37981; WBGene00033199; Cbr-ced-12.
DR   eggNOG; KOG2999; Eukaryota.
DR   HOGENOM; CLU_339579_0_0_1; -.
DR   InParanoid; A8XEZ1; -.
DR   OrthoDB; 234725at2759; -.
DR   Proteomes; UP000008549; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0019899; F:enzyme binding; ISS:UniProtKB.
DR   GO; GO:0070064; F:proline-rich region binding; ISS:UniProtKB.
DR   GO; GO:0044877; F:protein-containing complex binding; ISS:UniProtKB.
DR   GO; GO:0017124; F:SH3 domain binding; IEA:UniProtKB-KW.
DR   GO; GO:0031532; P:actin cytoskeleton reorganization; ISS:UniProtKB.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0016477; P:cell migration; ISS:UniProtKB.
DR   GO; GO:0060097; P:cytoskeletal rearrangement involved in phagocytosis, engulfment; ISS:UniProtKB.
DR   GO; GO:0043652; P:engulfment of apoptotic cell; ISS:UniProtKB.
DR   GO; GO:0035262; P:gonad morphogenesis; ISS:UniProtKB.
DR   GO; GO:0006911; P:phagocytosis, engulfment; ISS:UniProtKB.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR024574; ELMO_ARM.
DR   InterPro; IPR006816; ELMO_dom.
DR   InterPro; IPR001849; PH_domain.
DR   Pfam; PF11841; DUF3361; 1.
DR   Pfam; PF16457; PH_12; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS51335; ELMO; 1.
PE   3: Inferred from homology;
KW   Apoptosis; Cytoplasm; Phagocytosis; Reference proteome; SH3-binding.
FT   CHAIN           1..740
FT                   /note="Cell death abnormality protein 12"
FT                   /id="PRO_0000379435"
FT   DOMAIN          348..494
FT                   /note="ELMO"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00664"
FT   REGION          555..690
FT                   /note="Required for punctate localization, cell corpse
FT                   engulfment and distal cell tip migration"
FT                   /evidence="ECO:0000250"
FT   MOTIF           724..727
FT                   /note="SH3-binding"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   740 AA;  83972 MW;  D04A3036ED2719B8 CRC64;
     MPSTSLPYTQ MAFHMPLKEL QPVDTSLPEH IIKGAVVIDK ELTTWNRRAV IPSTALHTVF
     ITINRLEQKQ ADVVKMAARE MNLPEDNTYG LMADAPKRFI TNENIDQLGS GFILTLCASP
     DNYVKRITEI LEDGKNIAQM ENAVKTLDEF SLDPALIEAF YRCSSLELLF DLVRDDRVSM
     SYTLLSTCLR ALSSILELAV GDVTWKSVPR DVVVSIAALV TGKAKREEVN TLLAALAMIE
     QLVIGDDTTR DWVLEEVPIE TLIRHVEKSD ERIALAALSL MNSMIRHCSD KDKRLELIES
     LEVVPFRNAV HSSLLRDGSA RDPKALEQLV EVQRSLISAY DTSPASDSEI QKVLDIDSAN
     ENSEEDVEIW RTKLAEHRCG RLATVAMVLF GEKSPQDLRM LISENTMRIE GGKWQLIPMW
     MRCCDITAEL FGLLPGRDEL DRLISIIFST DSPFPAVFSC IVHLFHRTWR EMQAKGGEMD
     KVASFVLEQL RHVLKRKEIH DVEEMSADLE TFSYKAMQEV RREEQLEKEN DQLHSEAVIS
     LKAKLRPKIE ELVRINHLNY LKKGDVFRKP MKSKSLAKAA YWFWKLDASE KMLTITACDG
     ERFVDDGHRD DIRQVWLKDV ADVTNNDEID RKASSSRFAS SPSTNMLRGV RVQLKPTNDL
     KEGEVLMALT PDETQAGIWQ ESLAYLVGNT EMRSKTNAIV ERMLKMELRV RLLNVKLADP
     ENEPDVPPIP DDLISFISSF