ID   CEDB_SULAC              Reviewed;         597 AA.
AC   Q4JAQ5;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=DNA import protein CedB {ECO:0000305};
DE   AltName: Full=Crenarchaeal system for exchange of DNA protein B {ECO:0000303|PubMed:26884154};
GN   Name=cedB {ECO:0000303|PubMed:26884154};
GN   OrderedLocusNames=Saci_0748 {ECO:0000312|EMBL:AAY80124.1};
OS   Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC
OS   15157 / NCIMB 11770).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Sulfolobus.
OX   NCBI_TaxID=330779;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX   PubMed=15995215; DOI=10.1128/jb.187.14.4992-4999.2005;
RA   Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E.,
RA   Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.;
RT   "The genome of Sulfolobus acidocaldarius, a model organism of the
RT   Crenarchaeota.";
RL   J. Bacteriol. 187:4992-4999(2005).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE, AND
RP   MUTAGENESIS OF LYS-292.
RC   STRAIN=JDS22, and MW001;
RX   PubMed=26884154; DOI=10.1073/pnas.1513740113;
RA   van Wolferen M., Wagner A., van der Does C., Albers S.V.;
RT   "The archaeal Ced system imports DNA.";
RL   Proc. Natl. Acad. Sci. U.S.A. 113:2496-2501(2016).
CC   -!- FUNCTION: Part of the Ced system, which is involved in DNA import.
CC       {ECO:0000269|PubMed:26884154}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:26884154};
CC       Single-pass membrane protein {ECO:0000255}.
CC   -!- INDUCTION: Up-regulated upon UV stress. {ECO:0000269|PubMed:26884154}.
CC   -!- DISRUPTION PHENOTYPE: DNA transfer is completely abolished in deletion
CC       mutants. Deletion does not reduce cellular aggregation induced by UV
CC       stress. {ECO:0000269|PubMed:26884154}.
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DR   EMBL; CP000077; AAY80124.1; -; Genomic_DNA.
DR   RefSeq; WP_011277626.1; NC_007181.1.
DR   AlphaFoldDB; Q4JAQ5; -.
DR   SMR; Q4JAQ5; -.
DR   STRING; 330779.Saci_0748; -.
DR   TCDB; 9.A.53.1.1; the crenarchaeal system for exchange of dna (ced) family.
DR   EnsemblBacteria; AAY80124; AAY80124; Saci_0748.
DR   GeneID; 3473279; -.
DR   KEGG; sai:Saci_0748; -.
DR   PATRIC; fig|330779.12.peg.717; -.
DR   eggNOG; arCOG05935; Archaea.
DR   HOGENOM; CLU_516412_0_0_2; -.
DR   OMA; DEAWRIS; -.
DR   Proteomes; UP000001018; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..597
FT                   /note="DNA import protein CedB"
FT                   /id="PRO_0000437448"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         286..293
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000305"
FT   MUTAGEN         292
FT                   /note="K->A: Abolishes DNA transfer."
FT                   /evidence="ECO:0000269|PubMed:26884154"
SQ   SEQUENCE   597 AA;  68901 MW;  5016E9E31FB436A1 CRC64;
     MNKAFIVVAV VLLILGIISF NLVFIILAII SLFFVDPQIM RKFYKFFLKS NISKIFVKNY
     KTNHSIVIED GYLKIEDNVK AFLIVDDIPF DYRDLSDESL RVKISSFHKV LDIAGQIDIV
     FRKSSIDKNK FLSDLFQKAQ NIRVIIDADP SNERAKNELM MIQHMIKKIS EGEMPFKYLI
     FFIINSDSKE KALATADVVK KGLESIGVKS RLAYKHEIED LLNDKLSLKK IVFPSQIPFL
     SVFSLQKQPD YEIITDGIYL GQEINDRRAV FWNVNRVINP HALIIGPTGS GKTEFLLSLG
     VKTNILYGIP IVFFDVKKDI SLRLKKYGYK YKYINPLLNS INLLKFSNVN KDIYLIQLEN
     IIRNSFKLDR FVSALLYRIL LESISDNYYE VSWDYIIDKI EKYDINEDVK AYLLRIVSAI
     KSLDAGVEDI DLISAISEGI NVVDLSSIKS EELRRLVMYG IIIKFINKYN IADDRLKLVL
     VIDEAWTLLR SEDRDYQIVA DLIKRGRGFG IGIFMATQNF DDLGELSDIF LENIGLLGFM
     NNGDKKFWNE VMRFADLNIE ETLRSLIFLG KGEMLIRFIN DPRPIMIKTD VLVRNSF